EMAGE mouse gene expression spatial database

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EMAGE:21513

Polr3e polymerase (RNA) III (DNA directed) polypeptide E ( MGI:1349452)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001113_01	euxassay_001113_02	euxassay_001113_03	euxassay_001113_04	euxassay_001113_05

euxassay_001113_06	euxassay_001113_07	euxassay_001113_08	euxassay_001113_09	euxassay_001113_10

euxassay_001113_11	euxassay_001113_12	euxassay_001113_13	euxassay_001113_14	euxassay_001113_15

euxassay_001113_16	euxassay_001113_17	euxassay_001113_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21513_wholemount_strong.wlz

21513_wholemount_moderate.wlz

21513_wholemount_weak.wlz

21513_wholemount_possible.wlz

21513_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21513_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 4 5 1 2 3 4

foregut-midgut junction

weak

regional

weak expression: see section 8 9 0 1

stomach

weak

regional

weak expression: see section 1 2 3 4 5

hindgut

weak

regional

weak expression: see section 8 9

midgut

weak

regional

weak expression: see section 4 5 6 7 8 9 0 1

kidney calyx

weak

homogeneous

weak expression: see section 5 6 1 2 3 4 5

chondrocranium

weak

regional

weak expression: see section 2 3 6 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4649
Entity Detected:	Polr3e, polymerase (RNA) III (DNA directed) polypeptide E ( MGI:1349452)
Sequence:	sense strand is shown >T4649 CCACGCGTCCGCCCACGCGTCCGGGCCGCTGCCTGATAGAAGACCCGGCCGCCGCCGTCTCTGCAGCCCG CGGGTCCCTGGGCCGTTCCGCCGCCCGCGCGCGGCCCCGGCGGAGAGATCGAGTTGAGGACTGTGAGGTG GGCTCCCTAGTATGGCCAATGAAGAAGATGACCCAGTCATACAGGAGATCGATGTGTACTTGGCTAAAAG TCTGGCAGAGAAGCTGTATCTCTTTCAGTATCCTGTGCGTCCTGCTTCAATGACCTATGATGACATTCCA CATCTCTCAGCCAAGATCAAGCCCAAACAGCAGAAGGTAGAGCTTGAGATGGCCATCGACACCCTGAATC CCAACTACTGCCGCAGCAAAGGGGAGCAGATCGCACTGAACGTGGACGGTGCCTGTGCTGACGAGACTAG CACATATTCCTCAAAGCTGATGGACAAGCAGACCTTCTGCTCCTCCCAGACCACCAGCAACACAGCTCGT TATGCCGCTGCACTCTACAGGCAAGGTGAGCTCCACCTGACACCTTTACATGGCATCCTACAGCTTCGGC CCAGCTTCTCCTACCTTGATAAGGCAGATGCCAAGCACCGAGAGAGGGAGGCAGCCAATGAGGCAGGGGA TTCTTCTCAGGACGAGGCAGAAGAAGACGTTAAGCAGATCACGGTACGGTTCTCCAGGCCAGAGTCCGAG CAGGCTCGCCAGCGCCGCGTGCAGTCCTATGAGTTCTTGCAGAAGAAGCATGCAGAAGAACCCTGGGTCC ACCTGCACTACTATGGCATGAGGGACAGCCGCTCAGAGCATGAGCGCCAGTACCTGCTGTGTCAGGGATC CAGCGGGGTAGAGAACACGGAGCTCGTCAAGTCACCCAGTGAATACCTCATGATGCTCATGCCGCCCAGC CCAGAGGAGGAGAAGGACAAGCCTGTGGCTCCCAGCAATGTCTTATCTATGGCCCAGCTGCGTACATTGC CTCTGGCTGATCAGATTAAGGTCCTGATGAAGAACGTGAAAGTCATGCCCTTCGCTAATCTCATGAGCCT CCTGGGCCCCTCTGTGGACTCTGTGGCTGTTCTACGTGGCATCCAGAAGGTGGCGATGTTAGTCCAAGGA AACTGGGTGGTAAAGAGTGACATCCTGTATCCCAAGGATTCTTCCAGCCCTCACAGTGGCATGCCTGCTG AGGTGCTCTGCAGGGGCCGAGACTTTGTTATGTGGAAGTTCACACAGAGCCGATGGGTGGTAAGAAAGGA GGTGGCAGCAGTGACCAAACTCTGCGCTGAGGATGTGAAGGACTTTCTGGAGCACATGGCTGTAGTGAGA ATCAACAAAGGCTGGGAGTTCTTACTGCCTTACGACCTGGAGTTTATCAAGAAGCATCCAGATGTGGTCC AGCGGCAGCACATGCTGTGGTCGGGCATCCAGGCCAAATTAGAAAAAGTCTATAATCTCGTAAAAGAAAC CATGCCAAAGAAGCCGGATGGACAATCAGCGCCTGTTGGTCTGGTCTCTGGGGAACAGCGGGTCCAAACA GCCAAAACCAAGGCCCAGCAGAACCATGCCTTCTTGGAGCGTGAACTGCAGCGACGGAAGGAGCAGATGC GGGCAGCCACAGTCCTGCCCAGCGTGCAGATCAAGGAGGAACCCCTGAGTGAGGAGGAAGCCGATGGGGC TGAGCTGGAGGCTGAGGAAGAGGAGCCCATGGACACTGCACCCAGCACTTGCCTCAGCACCAAGTTGGCC AATGGGCTGCCTGCTGGGCGGGCAGTGGGTGGGGACAGCCTAAATGGACACCCAGTTCCAGGCTGTGCCA GCAACCCGGTTGCCTGTGAACTGAAGGCTTTTGTGGAAGCCACCTTTCAGAGACAGTTTGTGCTCACGCT GAGCGAACTCAAGCGCCTCTTCAACCTGCACCTGGCAGGCCTGCCCCCGGGCCATATACTCTTCAGTGGC GTCTCAGACCGAATGCTACAGGATACGGTGCTGGCCGCTGGCTGCAAGCAGATACTGGTGCCTTTTCCCC CACAGACTGCTGCTTCCCCTGATGAGCAGAAGGTATTTGCCCTCTGGGAGTCTGGAGACATGAGTGACCA GCATCGGCAGGTTTTGCTTGAAATATTTTCCAAAAATTACCGAGTACGTCGGAACTTGATCCAGTCTCGG CTGACTCAAGAATGTGGAGAAGAGCTAAGCAAACAGGAGGTGGATAAAGTGCTAAAGGACTGTTGTGTAA GCTGTGGTGGCATGTGGTACCTTAAGGGGACAGTACAATCCTGACAATAGGAGCAAACTGCTAACTCGAC GAATCCAAGCCCAGGGAAGGATGGCAGAGCCACACGTAGTCTTGACTTGCCTCAAGACACAGGAAGAGGA TCTGCCTACCTGGTGGCCTGTGGCGCTGCCTGGCCCAGAGGACAGAGGGGCTCATGCTCAGCCAGTGGCA GGGTCAGCGTAAGTGAGAGCGCTCCCAGCAGAGACCAGCGGGCCTTCTTTGCAGTACAATTTGAAATTCC TGATGTATTTTGCTTATTGGGTTTCATTCTCATAATAAAGAGAGTGTACACTGACATGGGCAGGATTACG AAAATCATGGTTTAATATTTTCCTCTTTTAAATGAATGCCAACATAGTTAAAGATATGTTTACAAGATGA AGAGAACCTTAAGGTTTTTAATATTTAAAATGCCTAGAAGCCAATAATTAGTCTTAGAGTTTCTCTCTGC TAAGACTCCTGCCAATTTTATTAAACCAAATTGAACATTTTACTGTTGGGTTTCTGAGGCCACTTTACCT TTATAGCAGCCTGCTTTCTGTAGCGGTCTCAACTGTTTACACAAACCATAGCAAAACATTAGATTAACTC CATCTTCTCCTAATGTTTGCATAAAGGTGCAAACTAAACCAGGTAAGTGTGGAACAGTATATAAGCGAGG TTATCACTTTGATGTAAAAAAATCTATTTTGTGTATTTTTAAAATAAATGCTAACACTAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5010681 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010681 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001113 same experiment
	EMAGE:21515 same embryo
	EMAGE:21517 same embryo
	EMAGE:21518 same embryo
	EMAGE:21514 same embryo
	EMAGE:21516 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source