Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29506

Uxt ( MGI:1277988)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506
euxassay_006893_01 euxassay_006893_02 euxassay_006893_03 euxassay_006893_04 euxassay_006893_05
EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506
euxassay_006893_06 euxassay_006893_07 euxassay_006893_08 euxassay_006893_09 euxassay_006893_10
EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506
euxassay_006893_11 euxassay_006893_12 euxassay_006893_13 euxassay_006893_14 euxassay_006893_15
EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506 EMAGE:29506
euxassay_006893_16 euxassay_006893_17 euxassay_006893_18 euxassay_006893_19 euxassay_006893_20
EMAGE:29506 EMAGE:29506 EMAGE:29506
euxassay_006893_21 euxassay_006893_22 euxassay_006893_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7975
Entity Detected:Uxt, ( MGI:1277988)
Sequence:sense strand is shown

>T7975
GTACTCCTGTACCGGTGCCCGTCATGGCGACGCCCCCGAAACGGCGGGCCTTGGATACGGTGGGGGAGAA
AGTGCTGCGGTACGAGACCTTTATCAGTGACGTACTGCAGCGAGACTTGCAAAAGGTGCTGGATCATCGA
GACAAGGTATATGAGCAGCTGTCCGTATATCTTCAACTAAGAAATGTCATTGAGCGACTCCAGGAAACTA
ATCACTCGGAGTTATATATGCAGGTGGATTTGGGCTGTAACTTCTTCGTTGACACAGTGGTCCCAGATAC
TTCACGCATCTATGTGGCCCTGGGATATGGTTTTTTCCTGGAACTGACACTGGCTGAAGCACTCAAGTTC
ATTGACCGAAAGAGTTCTCTCCTCACAGAGCTCAGCGACAGCCTCACCAAGGACTCCATGAATATCAAGG
CCCATATCCACATGATGCTAGAGGGACTTAGAGAACTACAAGGCCTGCAGAATTTCCCAGAGCCATCTCC
CCATTGACTGCATCTTCCCAGCCTCCAATATTAAAGCACCTGAATGCCTTGGAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30290324 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30290324 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006893 same experiment
 EMAGE:31480 same embryo
 EMAGE:31484 same embryo
 EMAGE:31366 same embryo
 EMAGE:31155 same embryo
 EMAGE:31159 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS