EMAGE mouse gene expression spatial database

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EMAGE:30203

Rnf10 ring finger protein 10 ( MGI:1859162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003565_01	euxassay_003565_02	euxassay_003565_03	euxassay_003565_04	euxassay_003565_05

euxassay_003565_06	euxassay_003565_07	euxassay_003565_08	euxassay_003565_09	euxassay_003565_10

euxassay_003565_11	euxassay_003565_12	euxassay_003565_13	euxassay_003565_14	euxassay_003565_15

euxassay_003565_16	euxassay_003565_17	euxassay_003565_18	euxassay_003565_19	euxassay_003565_20

euxassay_003565_21	euxassay_003565_22	euxassay_003565_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6702
Entity Detected:	Rnf10, ring finger protein 10 ( MGI:1859162)
Sequence:	sense strand is shown >T6702 GAAGGCGCTGGCGGGCAGTGATGGCGGCGGGTGATGGGGACGTAAAGCTAAGCACCCTGGGGAGCGGCGG GGAGAGTGGCGGCGACGGGAGCCCGGGTGGCGCCGACCCCCGCCGGCCCTGAACGCCATGAGCCTGGGTC CCCGCCGCGCCCGCTCCGCTCCGACCGCCGTCGCCGCCGAGGCCCCCTTTGATGCCGCAGAGCTCCCCCA GCGCCGCCGCCACCGCCTCCGACATGGACAAGAACAGCGGCTCCAACAGCTCCTCCGCCTCCTCGGGCAG CAGCAAAGGGCAACAGCCGCCCCGCTCCGCCTCGGCGGGGCCGGCCGGCGAGTCTAAACCCAAGAGTGAT GGAAAGAACTCAAATGGATCCAAGCGTTACAATCGCAAGCGTGAACCCTCCTACCCCAAAAATGAAAATT TTAGCAACCAGTCCCGTCGCTCCAATTCACAGAAAAGCAAAACTTTTAACAAGATGCCTCCTCAAAGGGG CGGTGGCAGCAGCAAACCCTTTAGCTCTTCTTCTAATGGTGGAAGACGAGATGAGGTAGCAGAGGCTCAG CGGGCAGAGTTTAGCCCTGCCCAGTTCTCTGGCCCTAAGAAGATCAACCTGAACCACTTGTTAAATTTCA CCTTTGAACCACGTGGCCAGGCAGGTCACTTTGAAGGCAGTGGGCATGGCGGCTGGGGGAAGAGAAACAA GTGGGGACACAAGCCTTTCAACAAGGAGCTCTTTCTACAGGCCAACTGCCAATTTGTGGTGTCTGAAGAC CAAGACTACGCAGCTCACTTTGCTGACCCTGACACCTTAGTCAACTGGGACTTTGTGGAACAAGTGCGCA TCTGTAGCCATGAAGTGCCATCTTGCCCAATATGCCTCTATCCACCTACTGCGGCCAAGATAACGCGCTG TGGACACATTTTCTGCTGGGCATGCATCCTGCACTATCTTTCACTCAGTGAGAAAACCTGGAGCAAATGT CCCATCTGCTACAGCTCTGTGCATAAGAAGGATCTCAAGAGTGTTGTTGCCACAGAGTCTCGACAGTATG CTGTGGGGGACACCATTACCATGCAGCTGATGAAGAGAGAGAAGGGAGTGTTGGTGGCCCTGCCCAAATC CAAGTGGGTGAATGTAGACCATCCTATTAACCTAGGAGATGAGCAGCTCAGCCAGTATTCCAAGCTGCTG CTGGCCTCGAAGGAGCAGGTGCTGCACCGGGTAGTTCTGGAGGAGAAAGGAGCGCTGGAGCAGCAGCTGG CAGAGGAGAAGCACACTCCTGAGTCCTGTTTCATTGAGGCTGCTATCCAGGAGGTCAAGATTCGGGAAGA AGCTCTGTCAGGAGTGGCTGGAGGCGGAGGGGAGGTCACTGGTGTTGTGGCTGCTCTGGAACACCTGGTG CTGATGGCTCCCCTGGCCACGGAGTCTGCTTTCCAACCCAGGAAGGGTGTGCTGGAGTATCTGTCTGCGT TTGATGATGAAGCTGCACAAGTTTGTTCTCTGGACCCCCCTGGTCCTCTTGCCCTCCCTTTGGTAGAAGA GGAGGAAGCAGTGTCTGAGCCAGAAGCCTGTGAGGACGCAGAGGTAGCCGATGACAGTCTGGGAGAGGGG ACCGTTGGTCCTGAGATGAGCCAGGAGGAGCCCATCACTAAGCCAGGCTTCACCCAGCTCAGCAGCTCTC CGTGTTACTACTTCTACCAGGCGGAAGATGGACAGCACATGTTCCTGCACCCTGTCAACGTGCGCTGCCT TGTGCGGGAGTACGGCAGCCTGGAGCAGAGCCCAGAGAAGATCTCAGCAACCGTGGTAGAGATTGCTGGG TATTCCATGTCTGAGGATGTTCGGCAGCGTCATAGGTATCTCTCTCACTTGCCACTCACCTGTGAGTTCA GCATCTGTGAACTGGCTCTGCAGCCTCCTGTGGTCTCTAAGGAAACCCTAGAGATGTTCTCAGATGACAT TGAGAAAAGGAAGCGTCAGCGCCAAAAGAAGGCTCGCGAGGAGCGTCGCCGAGAGCGCAGAATTGAGATG GAGGAGAACAAGAGACAGGGCAGGTATCCCGAGGTCCACATTCCCCTCGAGAACCTACAGCAGTTTCCGG CCTTCAATTCCTATACCTGTCCCTCTGATTCTGCTTTGGGTCCCACCAGCACTGAGGGCCATGGGGCCCT ATCCCTGTCTCCTCTGAGCAGAAGTCCAGGCTCCCATGCAGACTTTCTGCTGACCCCTCTGTCACCCACT GCCAGTCAGGGCAGTCCTTCCTTCTGCGTTGGGAGTCTGGAAGACGACTCTCCCTTCCTTTCCTTTGCCC AGATGCTGAGGGTTGGCAAAGCAAAAGCAGACGGGTGGCCCAAAACTGCTCCAAAGAAAGATGACAATAG CTTGGTTCCTCCCGCCCCTGTGGACAGTGATGGGGAGAGTGACAATTCTGACCGTGTTCCTGTGCCCAGT TTCCAAAATTCCTTCAGCCAAGCTATCGAAGCAGCCTTCATGAAATTGGACACACCAGCTACTTCAGACC CCCTCTCTGAAGACAGAGGAGGGAAGAAAAGGAAGAGACAGAAGCAGAAGCTGCTGTTCAGCACCTCGGT CGTCCACACCAAGTGACACTATTGCCCAGGCGCCTTCTCCATCGGGTTTCCTCTCTGTGCTTTTGTTTTG CTGCTGTAATTTTTAAGTATTTGAGTTTGAACAGATTGATTAGCTTGGGGTGGGGGGAGGGGCTTTCCCC AAAGTGAGGGGGAACCAAGAAAATTTTAAATACAGTGTATTTTCCAGCTTCCCCTCTTTACCCCAGAATA AAGTATTGGCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602969 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602969 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003565 same experiment
	EMAGE:31182 same embryo
	EMAGE:30377 same embryo
	EMAGE:30189 same embryo
	EMAGE:30376 same embryo
	EMAGE:30378 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source