EMAGE mouse gene expression spatial database

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EMAGE:30372

1700029I01Rik RIKEN cDNA 1700029I01 gene ( MGI:1917255)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004427_01	euxassay_004427_02	euxassay_004427_03	euxassay_004427_04	euxassay_004427_05

euxassay_004427_06	euxassay_004427_07	euxassay_004427_08	euxassay_004427_09	euxassay_004427_10

euxassay_004427_11	euxassay_004427_12	euxassay_004427_13	euxassay_004427_14	euxassay_004427_15

euxassay_004427_16	euxassay_004427_17	euxassay_004427_18	euxassay_004427_19	euxassay_004427_20

euxassay_004427_21	euxassay_004427_22	euxassay_004427_23	euxassay_004427_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7578
Entity Detected:	1700029I01Rik, RIKEN cDNA 1700029I01 gene ( MGI:1917255)
Sequence:	sense strand is shown >T7578 TAGTGCCTAGTATTTGCGGTGCCTGAAAACTTTCTTAAGAAGCAGTTGTTTTGCTGGCCTCCTAGGACAT CCAGGAGCTGGTCCTGTCATCTTCCAGGTATTCATATGCTCAGCTAAATGTACATAACTGTAAATCAACA CTAAGAGCACTGGATAGTTAAGGTCAAGGAGATTCTCACAAAGGATCCAGAAGAGTTCCAATATTTGAAA ATGGAAAAAAAAAGAAAAAAAAGAAAAAAAAAAGAATGAGTTCTTCTCTGGTAAAATTCCAGGTGGAAGA AGGGGAAATGCAAACTTGGTGTTCTGAGACTCCCTTAGAGACCCTGGGAGAAGGACAGCAACAAGAATGA GTGTGTGTCTGGTAAATACACCACAGGGTCTGTTAACCTTCAAGGACGTGGCCTTGGACTTCTCACTGGA GGAATGGGAATCTCTCAGTTTTGCTCAGAGGACATTGTACATGGATGTGATGTTGGAGAATTACAATAAT CTGTTATTTGTGGAAAATCATTGCATATGTGGAAAGTATGAGAAGGTCTTGGATCAAGACAGCCAGTACA TTGTCCATGAACACATGAATATTCAAGAGAAGTCTTCTAAATGGGACAAACTTAGCAATGTGATACTTGA AGCCCCCCAATGTACACCTTACAAAACTAATCACACCTCTGATGCTCTTCAATTTTCGAACCAAAAAAGA CTTAAGCCCAGGAACACTAAAGAAGTTTGCAAATACAATGACTCTGTAAACAGTTTAACTTTGTTTTCTA CCATTAGTCTCAATCAGGGAATCCACATGCAGAAGAAAAAACACAACGGAAATGCAGAATCTGAGAAGGC TTTTATTTCTAAACATAAAGTCATGGTGAAACGAAATAATACTGGAGTGAACCCTTACAAATGTAGTAAA TTTGACAAATATCTTACCCAGAGAGACAAACTTCAAAGTCAGCAGAGAATTTATCATGAAAAGAAACATT ACAGAAGTAGTAAAAGTGACAAATGCTTTACCCATCAGATCGATCTTAGTATCCATCAGGGAATTCATGC AGAAGAGAAAATTTATAAATGTAGTGAATGTGACAAATGCTTTACCCACAAATTTCATCTTAATATTCAT CAGAGAATTCATACAGGAGAGAAACCTTACAAATGTAGTGAATGTGACAAATGCTTTACCCAAAAATCTC ATCTTAATATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAAATGCATTGTATGTGAGAAATGCTT TACTGAAAAAGGCAATCTGAGAATTCATCAGAGGATTCATACAGGAGATAAACCTTACAAATGCAGTGAA TGTGACAAATGCTTTACCCAAAAATCCCATCTTAATATTCATCAGAGAATTCATACAGGAGAGAAACCTT ACAAATGTAGTCAATGTGACAAATCCTTTACTGAAAATGGCCATCTGAGAATTCATCAGAGAAATCATAC AGGTGAGAAACCTTACAAATGTAGTCGATGTGACAAATCCTTTACTGAAAATGGCCATCTGAGAATTCAT CAGAGAATTCATACAGGAGAGAAACCTTACAAATGCAGTGAATGTGACAAATGCTTTACTAAAAAATTCA GTTTGAGGATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAAATGCAGTGAATGTGACAAATGCTT TACTTTCAAAGGCAGTCTGAGGATTCATCAGAGAATTCATACAGGAGAGAAACCCTACAAATGTAGTGAA TGTGACAAATCCTTTACTGAAAATGGCTGTTTGAGAATTCATCAGAGAATTCATACAGGAGAGAAACCTT ACAAATGCACTGAATGTGACAAATGCTTTACTTTCAAAGGCAGTCTGAGGATTCATCAGAGAATTCATAC AGGAGAGAAACCTTACAAATGCACTGAATGTGACAAATCCTTTACTGAAAAGGGCAAACTGAGAATTCAT CAGAGAATTCATAAAGGAGAGAAACCTTACAAATGTAGTGAATGTGACAAATGCTTTACTGAAAAAGGCC ATCAGAGAATTCATACAGGAGAGATAGCTTACAAATAGTTTGTATGTGACAAATACAATACAATGTCAGT CTGAGAAATCATCAGTTTTTAATACAGTAGAGAATATTTATAAATGAAGTGAATGAGACAATCCTTTACC AAGATATATCATCTTAAAAATCATTAGAGAATACTTACAGAAGAGAAACTTTTCAATTATATCGAAGGTA TGAATCCTTTACTCTTGGCCTATTTCCTAGAATGTTAACAGTGAATACTTACAGGATACAAACTTAACTC ATGCATTGTATGAAGAAATATCTTTGACTTCATTTCAGGTCAAAGAGAATATCTAATAACTTTTTAGGCA AAGTATTTCTGTTGGGGAAAGTGTGGCCTGTGTTTTATTCACGTTCTATTCTTGACAACAATTGAGGCTC CACACTAGAGAATTAGTATGATTATGAAAATCATGTGAAAACTTTCATGCAATGTTCAAATCTTAAATAA TGTCAACTATTTTTTGCTTGAAGAAACTCTGAAAATGTAAAAATGTAGAAAGCTTTTCATGACACCTTTT TACTTGTTCACCCTTAAATGTTACAAGATGAAGACAAGTTGGGAAGTGAGGAGATATTATTGTGTAACAC ATTCATTGAGACAGAAATAATCCATTTCAAAAGGAAACTATTTAAGGTCTTTAATGCAGTCCTAAAAATA TCTAATAAAACTGGTAACATTCTGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242170 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242170 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004427 same experiment
	EMAGE:30371 same assay
	EMAGE:31417 same embryo
	EMAGE:30371 same embryo
	EMAGE:31481 same embryo
	EMAGE:31403 same embryo
	EMAGE:31457 same embryo
	EMAGE:31402 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source