EMAGE mouse gene expression spatial database

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EMAGE:30833

Scd2 ( MGI:98240)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007128_01	euxassay_007128_02	euxassay_007128_03	euxassay_007128_04	euxassay_007128_05

euxassay_007128_06	euxassay_007128_07	euxassay_007128_08	euxassay_007128_09	euxassay_007128_10

euxassay_007128_11	euxassay_007128_12	euxassay_007128_13	euxassay_007128_14	euxassay_007128_15

euxassay_007128_16	euxassay_007128_17	euxassay_007128_18	euxassay_007128_19	euxassay_007128_20

euxassay_007128_21	euxassay_007128_22	euxassay_007128_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5861
Entity Detected:	Scd2, ( MGI:98240)
Sequence:	sense strand is shown >T5861 GACACCGGTGGCTGCAAGCTGCGATTTTGGCGTCCTCTCATTTTCTATCCTTATCTCCGCCCGCGGCTGC CCTGGCCAGCCAGTTTTTGATTTTTTATCTGGTCATTGATCAATATAACGAACCTAAGGATATACAGGAC ATTAATACCCCACTGCCAGCTCTGGCCCAGAGCTTGTACCGCGCAGCGGGCTGCAGAAACTTAGTCATAG CACCCTTTGTCGCTGAGGTCTGAAGCTCGCTGCACGTTCTCATCCCTGGGAACGTGACCCCAGCATCCGA CGCCAAGATGCCGGCCCACATACTGCAAGAGATCTCTGGCGCTTACTCAGCCACCACCACAATCACAGCG CCACCTTCTGGGGGACAGCAGAATGGAGGCGAGAAGTTTGAAAAGAGTTCTCACCACTGGGGAGCAGATG TTCGCCCTGAACTAAAAGATGATCTATATGACCCCACCTATCAGGATGATGAGGGGCCCCCGCCCAAGCT GGAGTACGTCTGGAGGAACATCATTCTCATGGCCCTGCTGCATTTGGGAGCCTTGTACGGGATCACACTG GTTCCCTCCTGCAAGCTCTACACCTGTCTCTTCGCGTATTTGTACTATGTAATCAGCGCCCTGGGCATCA CAGCCGGGGCTCATCGCCTGTGGAGCCACAGAACATACAAGGCACGGCTGCCCCTGAGGCTCTTCCTCAT CATTGCCAACACCATGGCGTTCCAGAATGACGTGTATGAATGGGCCCGAGATCACCGCGCCCACCACAAG TTCTCAGAAACACACGCCGACCCTCACAATTCCCGCCGTGGCTTCTTTTTCTCTCACGTGGGTTGGCTGC TTGTGCGCAAACACCCGGCTGTCAAAGAGAAGGGCGGAAAACTGGACATGTCTGACCTGAAAGCCGAGAA GCTGGTGATGTTCCAGAGGAGGTACTACAAGCCCGGCCTCCTGCTGATGTGCTTCGTCCTGCCCACGCTG GTGCCCTGGTACTGCTGGGGCGAGACTTTTGTAAACAGCCTGTGCGTTAGCACCTTCTTGCGATACGCCG TGGTGCTCAACGCCACCTGGCTGGTGAACAGTGCCGCCCACCTCTACGGATATCGCCCCTACGACAAGAA CATTAGCTCTCGGGAGAACATCTTGGTTTCCATGGGAGCTGTGGGCGAGGGCTTCCACAACTACCACCAC GCCTTCCCCTACGACTACTCTGCCAGTGAGTACCGCTGGCACATCAACTTCACCACGTTCTTCATCGATT GCATGGCTCTCCTGGGCCTGGCTTACGACCGGAAGAGAGTGTCCAGGGCTGCTGTCTTGGCCAGGATTAA GAGAACTGGAGACGGAAGCTGCAAGAGCGGCTGAGTGTGGGGTCTTGCAGTTCCTGTTCCAGAAGCCAGC TTGGCAGAGGCTTACTGTTCTGTTAATTAACTACTGAGTATTGCCACCCAGATGCTAAAATGATGACTGA CGTTAACCCATTCTGGTACAGTATTGTAAAACGGACAAAAATCGGAAGTCGACAGCTCTTCCTTCTATGC TAAGCTCATCTTCCCTTCTAGTTTCTTTTCCCGTTGTCTTTTTCTTTGCTTTGTCCCTGATGACCTCGTT TCTCATTGCTTCTCCCAAGCCAGCCGCCGCTCAGCTATAGGTCAGTGTTCACCTTCCAGAGTACACCAAC TTCTCAAGGGTCTACAGTAAAGGTTCGTGCCCCACCCCCAACTCTTCTTAAGCTGCTCTGAGTTAGAAAT ACAAGAAAAATCTTGAGAGATTTCTCCTGTTATTTTTAGCCCAGGCTTTGCCAGATGGAATGAAAAAGAA AATAGTTTTTATTTGGCAGGGAGCCTATAAAAGAGGTAAATCTTAAGGGGCCAAGTTCAACACGCAGGCT ATGATTCATCAAAGCCGTGGGAGAGGGGGATCTCTTATGAACAGGCTCAGCTCCGTCTGGCAGAGCTACC GCAGCCTTTCAACATAGCGCGCCTCCTCTCCTAATTCTTAGTAGGTGGCAACTGAGGAACTTCGTGACAC TAAAATACAGTAACACATCAGTATCTCAGTGTAGTTTAGGCTGAAGATTTAAAAAATATATATATATATA TATACTAGTAAAGTGAAAAACAATTTGTAGGAAAAGTGGTCTTTAATAAGGATTTTCTTTTCCTTTAATG ACAAGGACCCGGGGCCAGGTGTGGTGGCTTGCATCTTAAATTGCAGGACTGGAGAGGCAGAGGCAGGTGG ATCTCTGAGTTCAAAGCCAGCCAGGGCTAGATAGTGAGACCTTGTCTCAAATTAAAAAAAACAACAGTTA GCAGGACATGCAGAGCAAGGGACCCTCGAGTGAGGCATGTTTGGAATCCGTAGGGCAGTCGCTCATGGTT TCGAGCGTCCCGTGAGCTGCACCCCCAGGGTCTTTTTAATTCCTGTTCTTCCATCTTTAAGATGATGGTC GTGCCCACAAATTGAGGATAGCTGTGGCGTTTCCATACATCTAACAGTGGAAGGATTTCAAGAGTTGAAC TTGAGTCACAGTTAAAACTGAAAAGTCGTAAAGTCTGTCCTGGATCCTTACAAACACTCCGTGAGTGCTT CCCTCAGAAGACGATGCTGCGCGCCTTCTCCCTCCCACTGTCGGGCAGTGGACGGGCACGTTGGAAGTCG CTGTGGGCGCCTCCTAATGACTTCTCAAAGGAAAGCTTCTGAGGGCCCCTTACCAGGGGACTTCACGGCT TTGGGGTGTTCTAGTGGAGCAGGTTAGGTTTTTTTTAGCCCCTTTCAGTCCCGCTGCTGTTTGTGGCAAG GCAGTACTGACTCATGCCTCACTAGCTCTGTGACCAGGTAGGACTCTGCCTGGGATACATGGTTGAAATC TCTCAGCTCTACCACACCTGCCTCTTTTCCCCTACCCATTTTTTGTGTTTTGGGGTGGGTTTTTTTGTTG TATTTCTTTTTTCCTCTTTGACATTTAGTCTTGGTCTAGGCAGAAATGTCCTGGATATTTCCAGGCAGTA AAGATGTGTGGTCCTGGCACCTTCCTCACTCAGAGATACAACCTCTATCTGCAGTAGGTAGAGACGGCTA TCCTTTTCTGGTGGGTTTCAGATTCTCCACCTCTTATCCCTCCCAGGCTTCTGACACTCTCTTCAGCTCT AAAGAAAAAGTTTGGCCAACTTTCTAGAAACATCCCTGGCTCAGAGAGACTCTTTGTCAGTCATTCCAAA ACAAATGGTTTGGTGGCACACATGCCGGCGAGTCAGGGCGGACAGAGCATGCACCGTGCTCCCGGCACTT GGCAAAGCTTCGGCAAAGCTTCGCGGGCTAATCAGCCCTCCTGGGTGATGCTGTGGTTAAGTGGGAAGCA CAGCACTCGAGGTTGTGTTCTTAAGTGCCTCTGTTTGAAAGCTTTGGGTAGGGTAGGAGAGCTGAGATGC CATGAATTTCACAGAAAGGCTCTCGTGCAGCAGTGCAGAAGCCTGGCCGCGTCGGCTGTACAGCATACCA GGTGCTGTGCTTCGAGGGGACAGTCTTCCTGGGCTAGTTGAATAGAGAGAGGCTTTAGGGCCTTCCTGTG CCCACTTGAAAGTAGCCTTACCCTTCAGCTGTTTGCTAAAAGAGAATTCTTGGGCGAATCCAAGGTATTT GCTGAGCTGTGGCCCTATTCTATTCCTTGGATATGCCTTTTGAGTGATTTCAGTTCCGCTGGTTATGGTG CCCACTTAAACAAATGTGAAAAATAAAAGCAGCTCTCTTCTTAGCCTGCCCCGTGGATCTGAACCATGCT GTGGCTAAGGACCCCGTAAAATAACATCTGTCGTTAAGGGTGTACCAGCTCACTAAGAAACACTGCTATC AAGATTTTTAAAGCTCAAACCAAGTGACACTTTTAATAAGAGACTAAGATCTGATTGAGGGAGTTTTCTA ACAGTCCTTGCCTCGTGGACACGCTAAAGTTTGACTTGGGTGCCTTACCTCTTTTCTAACCAGTGTTGCT CGTGAGCCTGTGGCGCTCGCTTGCTCGCTCGCTCGCTCGCTCCGTGGATTCCTTAAAACCAGAGAGCCTG TAAGTGTGGCAAGATCAAAACTGGCCTGGCTGGAGGGTTAGCTGGACATGGATCTGCAGGATTGCCTCTG GGGACCTCGTTTTGGGAACTTTTCTTGGGGCTGTTTTGCTCAAGTGCCCGTAGCTGATGGATGGGTGAAG TAATTTTGAACGTACGCGATTTGTAGGTTTTGTTATTTGTTTTAAGATTTAAAAGCTGGTTATAGGATTT TAAGTGAAAGCTTTTCTTCCTGGTTTGCTAGTGTTCTTGAGTGTCTTCTTTAGTTGAAATAGGTTAGTAG GAAATTTAAGAAAGTGGTGGCCGTGTTACACTGGTGGTGAAGGCCAGGGCCGCCTACCAACTCTCAGTTT TTTGTAACAGTCCCACTCTGACGATAATGAGTTCACTCCTTGGATAAGGATGTTAATGAGGGAAGAGCCT TCCCGACCTGACCTAATCAGCTCTTACTGGACCCAGCTGTCAACACTGTTGCATTGGGGATTTAAGTTTC CAACCCATGACTTATGGGACAGATTCAAACCGTAGCACTGGGCATTTCGCCTAACAATACTGTGACTCAG TATTCTGTGAAACTGGGATAATATTCCTGACCTACCTCCACGGGTAGGTGTGAGCATGAGAAAATGCTCT TTATAAAACATTTGGGGATCCTCTGCAGAGGAGTATTTAAGTCCTGCACATTTTTGTAGTAACTGTCCAT CGTGGACTTGACATGTGCCTATGTCCCAGATGACGTTGTTAATCTACATCATTCTCAGAGGTTGAATGGA TGCGTCAGATAATGTATACTCACTGCTATGAAAACATGCAGGACTCCGTTTCCTTCCTTGGGTGGGATTT TTCCTTTATTATGTGCAACTGTAGTTATTTGTAATCTAATAATTTTCATTAATACCAACTCCGAAAGTAT TTCTACTCATCTGAGGTTGTGTTTGTTCATAATAAAATGAAATGATCTGACTGCAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:3155869 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3155869 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007128 same experiment
	EMAGE:30164 same embryo
	EMAGE:30001 same embryo
	EMAGE:29951 same embryo
	EMAGE:31080 same embryo
	EMAGE:31149 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source