EMAGE mouse gene expression spatial database

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EMAGE:30941

Gpr65 ( MGI:108031)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006697_21	euxassay_006697_22	euxassay_006697_23	euxassay_006697_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6711
Entity Detected:	Gpr65, ( MGI:108031)
Sequence:	sense strand is shown >T6711 ACAAGGCAAGGTCTCTTTCCCTGCTGACAGCCTAAGAAGTGATGTAACTGCCACTGTGAAGACCATGGCG ATGAACAGCATGTGCATTGAAGAGCAGCACCACCTCGAGCACTATTTGTTCCCGGTGGTCTACATAATTG TGTTTATAGTCAGCGTCCCAGCCAACATCGGATCTTTATGCGTATCCTTTCTGCAAGCGAAGAAGGAAAA TGAGCTAGGAATTTACCTCTTCAGTCTGTCCCTGTCAGACCTGCTGTATGCGCTGACTCTGCCCCTCTGG ATCAATTACACTTGGAATAAAGACAACTGGACTTTCTCTCCCACTTTGTGCAAAGGAAGCGTTTTCTTCA CCTACATGAACTTTTATAGCAGCACAGCGTTCCTCACTTGCATTGCCCTGGACCGCTATTTAGCAGTCGT CTACCCTCTGAAGTTTTCCTTCCTAAGAACGAGAAGATTCGCGTTTATTACCAGCCTCTCTATCTGGATA TTAGAGTCCTTCTTTAACTCTATGCTTCTGTGGAAAGATGAAACGAGTGTTGAATATTGTGACTCGGACA AATCTAATTTCACTCTCTGCTATGACAAATACCCTCTGGAGAAATGGCAGATAAACCTCAACCTGTTTCG GACGTGCATGGGCTACGCAATACCCTTGATCACCATCATGATCTGCAACCATAAAGTCTACCGAGCTGTG CGGCACAACCAAGCCACGGAAAACAGCGAGAAGAGAAGGATCATAAAGTTGCTTGCTAGCATCACGTTGA CTTTCGTCCTATGCTTTACCCCCTTCCACGTGATGGTGCTCATCCGCTGCGTTTTAGAGCGCGACATGAA CGTCAATGACAAGTCTGGATGGCAGACGTTTACGGTGTACAGAGTCACAGTGGCCCTGACGAGTCTAAAC TGTGTTGCCGATCCCATTCTGTACTGCTTTGTGACTGAGACGGGGAGAGCTGATATGTGGAACATATTAA AATTGTGTACTAGGAAACACAATAGACACCAAGGACAAAAAAGGGACATACTTTCTGTGTCCACAAGAGA TGCTGTAGAATTAGAGATTATAGACTAAGAGGTGGAGGCAGGTTAAGTTACATGGTATTATTTAATGAAA CTTACATTTTGGAAAAGAAATCTGGCATAGTAGAACCCAGTGGAAATAGTTTGAAGGTACATTGTATGAC TCCTATGTTGGCTTTATTAAGTAAGGTATAGAAATGTATTATCTTGTATGTATTCTGATGACTAGGCATC ATTGTTTTAGTACCAATTCTCTTTGCCTCTATGCTATAACCCCTAAGCAGCACGCGGGACTGTTCGTGTT TAAATCAGTGGCCATTCTATCTGACTACTATGACTTTTTGTTGTTGTTCTGCTTTGGGTTTTCAGTCTGC CTGCATCAGTCTTCTCCTCTGTATACGCCTGTCTTCAACAAATGTAAGGACTAAATACCCCTCCCGATCA CATCCATTATCAAGGATTTGAAGCCACTCCATGTACTGGGTTATAAAAGAAATGTCTCATGAACTTTCAT GAAGTTTACATACCTTTGGGGATCTAGTCACCGAGTCACATAAAGTAAAAGTAAATGGATTTTGATTTTG GTCTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3661818 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3661818 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006697 same experiment
	EMAGE:30384 same embryo
	EMAGE:31593 same embryo
	EMAGE:30382 same embryo
	EMAGE:30035 same embryo
	EMAGE:31327 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source