EMAGE mouse gene expression spatial database

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EMAGE:30949

Tuba8 ( MGI:1858225)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002986_01	euxassay_002986_02	euxassay_002986_03	euxassay_002986_04	euxassay_002986_05

euxassay_002986_06	euxassay_002986_07	euxassay_002986_08	euxassay_002986_09	euxassay_002986_10

euxassay_002986_11	euxassay_002986_12	euxassay_002986_13	euxassay_002986_14	euxassay_002986_15

euxassay_002986_16	euxassay_002986_17	euxassay_002986_18	euxassay_002986_19	euxassay_002986_20

euxassay_002986_21	euxassay_002986_22	euxassay_002986_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4313
Entity Detected:	Tuba8, ( MGI:1858225)
Sequence:	sense strand is shown >T4313 GCAGGCCCAGCTGGGAGGCGAGCGGGCGAAGAGAGCGGCCACGATGAGGGAATGCATATCGGTCCATGTG GGTCAAGCCGGAGTTCAGATTGGCAATGCCTGCTGGGAGCTCTTCTGCTTGGAGCACGGCATCCAGGCGG ATGGAACCTTTGGCACTCAGGCCAGCAAGATCAACGATGACGACTCCTTTACCACCTTCTTCAGTGAGAC TGGCAACGGGAAACACGTGCCCCGGGCTGTCATGGTGGACCTGGAGCCTACCGTAGTAGATGAGGTGCGG GCAGGAACCTACCGCCAGCTCTTCCATCCTGAGCAGCTGATCACAGGCAAGGAGGATGCAGCTAACAATT ACGCCCGCGGGCACTATACGGTGGGCAAGGAGAGTATCGATCTGGTGCTGGACCGAATCCGTAAACTGAC TGATGCCTGCTCCGGCTTGCAGGGCTTCCTCATCTTCCACAGCTTTGGTGGGGGCACAGGATCTGGCTTT ACTTCTCTGCTGATGGAGCGCCTTTCCCTAGACTATGGCAAGAAGTCCAAGCTGGAATTTGCCATCTACC CAGCCCCCCAGGTCTCCACAGCAGTGGTGGAGCCTTACAACTCCATCCTGACCACCCATACCACCCTGGA ACATTCCGACTGTGCTTTCATGGTGGATAACGAAGCCATCTACGACATCTGCCGCAGGAACCTGGATATT GAACGCCCCACCTATACCAACCTCAACCGCCTCATCAGCCAGATTGTGTCCTCCATCACTGCCTCTCTCC GCTTTGATGGCGCCCTCAATGTGGACCTCACAGAGTTTCAGACCAACCTGGTACCCTACCCCCGAATCCA CTTCCCGCTGGTCACTTACGCCCCCATCATTTCTGCTGAGAAAGCCTACCACGAGCAGCTGTCTGTGGCA GAGATAACTAGCTCCTGTTTCGAGCCCAACAGCCAGATGGTGAAGTGTGACCCACGTCATGGCAAATACA TGGCCTGCTGCATGCTCTACCGCGGTGATGTGGTACCCAAGGACGTGAATGTCGCCATTGCTGCCATCAA GACCAAGAGAACTATTCAGTTTGTTGACTGGTGTCCCACAGGTTTCAAGGTGGGCATCAACTACCAGCCA CCTACTGTCGTGCCAGGAGGGGACCTGGCCAAAGTCCAGCGAGCCGTGTGCATGCTGAGCAACACCACAG CAATCGCAGAGGCCTGGGCCCGCCTTGACCATAAGTTTGACCTCATGTACGCCAAGCGGGCCTTTGTACA TTGGTATGTTGGAGAGGGTATGGAAGAAGGAGAGTTTTCTGAGGCCAGGGAGGACCTGGCTGCTCTGGAG AAGGATTATGAAGAAGTGGGGACTGATTCGTTTGAAGAAGAGAATGAGGGGGAGGAATTTTAAATATACA CTTGCCCCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4507364 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4507364 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002986 same experiment
	EMAGE:32154 same embryo
	EMAGE:31153 same embryo
	EMAGE:32138 same embryo
	EMAGE:32139 same embryo
	EMAGE:31199 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source