EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31045

Efhd2 EF hand domain containing 2 ( MGI:106504)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000316_21	euxassay_000316_01	euxassay_000316_02	euxassay_000316_03	euxassay_000316_04

euxassay_000316_05	euxassay_000316_06	euxassay_000316_07	euxassay_000316_08	euxassay_000316_09

euxassay_000316_10	euxassay_000316_11	euxassay_000316_12	euxassay_000316_13	euxassay_000316_14

euxassay_000316_15	euxassay_000316_16	euxassay_000316_19	euxassay_000316_20	euxassay_000316_22

euxassay_000316_23	euxassay_000316_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

strong

homogeneous

strong expression: see section 20 21 22

forebrain

strong

homogeneous

strong expression: see section 19

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1615
Entity Detected:	Efhd2, EF hand domain containing 2 ( MGI:106504)
Sequence:	sense strand is shown >T1615 CCACGCGTCCGGATTTCGACAGCAAACTCAGCTTCCGGGAGTTCCTTCTGATCTTCCGCAAGGCAGCAGC AGGGGAGTTGCAGGAAGACAGCGGCTTGCACGTCCTGGCCCGCCTGTCCGAGATCGATGTCTCCACAGAG GGCGTTAAGGGTGCCAAGAACTTCTTCGAGGCCAAGGTACAGGCCATCAACGTGTCCAGCCGCTTTGAGG AAGAGATCAAAGCTGAGCAAGAGGAAAGGAAGAAGCAGGCTGAGGAGGTGAAGCAGCGGAAAGCGGCCTT TAAGGAGCTGCAGTCCACGTTCAAGTAGCCAGAGCCAAGGCCGAGACCTGGCCCTGCCCCGTGTGCGGTC TGGGGGCACGGGTGGGTACAGGGGATCTGTGGGAGACTAGCTCCCAGGTCCTGCTCTCTGTGCCCGGACC ACTACTAAAAACCGCAAACGATATGTGACCCGATCTCATTCAGGAGTCTCCTCGGTGGTTGGTCCCTGCC CTGCCCTCTCCTGCGGTTCA
Notes:	The probe template was PCR amplified from IMAGE:2922781 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2922781 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000316 same experiment
	EMAGE:29318 same embryo
	EMAGE:31935 same embryo
	EMAGE:31904 same embryo
	EMAGE:31004 same embryo
	EMAGE:31940 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source