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EMAGE:31268

Acsl5 acyl-CoA synthetase long-chain family member 5 ( MGI:1919129)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268
euxassay_003738_01 euxassay_003738_02 euxassay_003738_03 euxassay_003738_04 euxassay_003738_05
EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268
euxassay_003738_06 euxassay_003738_07 euxassay_003738_08 euxassay_003738_09 euxassay_003738_10
EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268
euxassay_003738_11 euxassay_003738_12 euxassay_003738_13 euxassay_003738_14 euxassay_003738_15
EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268
euxassay_003738_16 euxassay_003738_17 euxassay_003738_18 euxassay_003738_19 euxassay_003738_20
EMAGE:31268 EMAGE:31268 EMAGE:31268 EMAGE:31268
euxassay_003738_21 euxassay_003738_22 euxassay_003738_23 euxassay_003738_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
oral epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
metencephalon
moderate moderate
regionalmoderate expression: see section 07
medulla oblongata
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13
oral region
moderate moderate
regionalmoderate expression: see section 05
metanephros
moderate moderate
regionalmoderate expression: see section 05 06 07 08 14 15 16 17
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 08
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
rectum
moderate moderate
regionalmoderate expression: see section 11 12
esophagus
moderate moderate
regionalmoderate expression: see section 11 12
pancreas
strong strong
regionalstrong expression: see section 09 moderate expression: see section 08 13 14
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 17
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 04 20 21 22 23 24
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 17
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 16 17 18
cervical ganglion
moderate moderate
regionalmoderate expression: see section 08 15 16
nasal septum
moderate moderate
regionalmoderate expression: see section 13 14 15
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 19 20 21 22 23 24
not examined not examined
regionalnot examined expression: see section 11
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09
urethra of male
moderate moderate
regionalmoderate expression: see section 11 12 13
midgut
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18
thyroid gland
moderate moderate
regionalmoderate expression: see section 09 10
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 13 14 15
submandibular gland primordium
strong strong
regionalstrong expression: see section 06 07 08 15 16 17 18 not examined expression: see section 19
adrenal gland
moderate moderate
regionalmoderate expression: see section 06 07 08 14 15
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11
pharynx
moderate moderate
regionalmoderate expression: see section 10 11 12
lung
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6966
Entity Detected:Acsl5, acyl-CoA synthetase long-chain family member 5 ( MGI:1919129)
Sequence:sense strand is shown

>T6966
TGCATGTCAGAAAGCCCGGCTATGGCCTGGCTGGGACAGATCAGGGCAGGGCAGAAGTGGGGCCACTAGC
CAGGCTGCCTGCCTGCCTGCCTGCATGGACGCTCTGAAGTCACCCTGCCCGCGGAGAAGCCCGGGGTGAG
GAAGGGAGGACCGGGGCTCTCCTGCCAGCAAGAAGCCGGAGCCAGAAAGCCTCACTCGGAAGCTCCAAGG
CGTGCGTGCGGCCCTTCCCAGGGTCTGAATTTCCTGTTGGTATTCACAAAGATGCTTTTTATTTTTAACT
TCTTGTTTTCCCCACTTCCAACTCCGGCCCTGATCTGCCTCCTGACGTTTGGAACGGCCATCTTCCTGTG
GCTGATCAACAGACCTCAGCCAGTTTTACCTCTCATTGACTTGGACAACCAGTCTGTGGGGATTGAGGGA
GGAGCACGGAGGGGCGCTTTCCAGAAGAACAATGACCTAATCCTTTATTACTTCTCAGACGCCAAGACGT
TGTATGAAAATTTCCAAAGAGGACTTGCTGTGTCTGACAATGGGCCTTGCTTGGGATACAGAAAGCCAAA
CCAGCCCTACAAATGGATCTCCTACAAACAGGTGTCTGATCGAGCAGAGTACCTGGGCTCCTGTCTTTTG
CATAAAGGATATAAGTCATCACAGGACCAATTTGTTGGCATCTTTGCTCAAAATAGGCCAGAGTGGGTCA
TCTCTGAGTTAGCCTGTTACACATATTCCATGGTAGCTGTCCCCCTGTATGACACGCTGGGTACAGAAGC
CATCATCTTTGTCATCAACAGGGCTGATATCCCTGTGGTCATCTGTGATACACCCCAAAAGGCAACAATG
CTAGTAGAAAATGTGGAAAAGGGCCTCACACCAGGCCTGAAGACGATCATCCTCATGGACCCTTTTGATG
ACGACCTGATGAAAAGAGGAGAAAAGTGTGGAGTTGAGATGTTATCCCTGCATGATGCGGAGAATATAGG
CAAAGAGAACTTCAAAAAACCAGTGCCTCCGAAGCCAGAGGATCTGAGCGTCATCTGTTTCACCAGTGGA
ACTACAGGTGACCCCAAAGGAGCTATGCTAACCCATGAAAATGTTGTTTCAAACATGGCTGCTTTCCTCA
AATTTCTGGAGCCTATCTTCCAGCCCACCTCTGATGATGTGACCATATCCTACCTTCCCTTGGCTCATAT
GTTTGAGAGGCTTGTCCAGGGTATCTTATTTTCCTGTGGAGGCAAAATTGGGTTCTTCCAAGGAGATATC
CGGTTGCTACCTGATGACATGAAGGCTTTGAAACCCACAGTGTTTCCTACAGTGCCTCGACTCCTTAACA
GGGTCTATGATAAGGTACAAAATGAAGCCAAGACACCTTTGAAGAAGTTCTTGTTGAACTTGGCTATCAT
CAGTAAATTCAATGAAGTGAAAAATGGCATCATTCGGCGGGACAGTTTGTGGGACAAGCTCGTGTTTTCA
AAGATTCAAGGAAGCCTGGGGGGGAAGGTTCGCCTCATGATCACTGGAGCCGCCCCCATCTCCACTCCAG
TCTTGACATTCTTCAGGGCTGCAATGGGATGTTGGGTGTTTGAAGCTTATGGCCAAACAGAATGCACAGG
TGGATGTTCAATTACATCACCTGGGGACTGGACAGCAGGTCATGTTGGGACTCCAGTGGCTTGCAATTTT
GTAAAGCTGGAAGATGTGGCTGACATGAACTACTTTTCAGTAAACAACGAAGGCGAGATCTGCATCAAAG
GTAACAATGTGTTCAAAGGCTACCTAAAGGACCCAGAGAAAACACAGGAAGTACTGGACAAGGATGGATG
GCTTCACACTGGGGACATTGGTCGCTGGCTTCCAAATGGAACTCTGAAAATTGTTGACCGGAAGAAGAAT
ATTTTCAAGTTGGCACAAGGAGAATACATTGCCCCAGAGAAGATTGAAAATGTTTATTCCAGGAGCAGAC
CAGTATTGCAAGTTTTTGTCCATGGGGAGAGCTTACGGTCATTCCTGATCGGAGTGGTGGTTCCCGACCC
AGATTCACTTCCCTCATTTGCAGCCAAGATCGGGGTAAAGGGATCATTTGAAGAACTGTGCAAAAACCAA
TGTGTAAAGGAAGCCATTTTAGAAGACTTGCAGAAAATTGGGAAAGAGGGTGGCCTGAAATCCTTTGAGC
AGGTCAAAAGTATCTTTGTGCATCCAGAGCCCTTCACTATTGAAAACGGACTTCTGACACCGACACTGAA
AGCCAAACGAGTAGAGCTTGCCAAGTTCTTCCAGACACAAATCAAGAGCCTCTATGAGAGCATCGAGGAG
TAGTAGCTTAAGTTTGCTGAGCTGGAAGCTGTGGGGGAAGAAGTGGAAACTATGTCCAGTGAACTTTTCC
AGTAAATGAAGCAAGCACTAAAGTACTGTCCTGAGTTGGGAACAAAGCACTGTGGGCAAGGTCGATGCAA
TGCCTGCACTACAGGCCTGTCTTCTGTGGATCTCTCTCCTTGGCCTTCAGTCCCTGCAGTATTTTACTGT
CAGATAGCATGTGGTGCCTCTACTTGTAAATGTCAAATCTTTGAAATAAACTATTACAGATAAAAAAAAA
AAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3987201 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3987201 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003738 same experiment
 EMAGE:29400 same embryo
 EMAGE:29425 same embryo
 EMAGE:31227 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS