EMAGE mouse gene expression spatial database

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EMAGE:31285

Aida axin interactor, dorsalization associated ( MGI:1919737)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007099_01	euxassay_007099_02	euxassay_007099_03	euxassay_007099_04	euxassay_007099_05

euxassay_007099_06	euxassay_007099_07	euxassay_007099_08	euxassay_007099_09	euxassay_007099_10

euxassay_007099_11	euxassay_007099_12	euxassay_007099_13	euxassay_007099_14	euxassay_007099_15

euxassay_007099_16	euxassay_007099_17	euxassay_007099_18	euxassay_007099_19	euxassay_007099_20

euxassay_007099_21	euxassay_007099_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8535
Entity Detected:	Aida, axin interactor, dorsalization associated ( MGI:1919737)
Sequence:	sense strand is shown >T8535 TGTGGCGGCAAATGGGTTTGGGCGGCTCCTCGGCGGGTGGCGGTGGTGGCCTTAGCGGTTCCTCCTGGCC CTGTTAATGTCAGGGCCAAGCCCGGGGAGGATGGCGCCCTAGAGCCTGGCCTTGCTGGGGTAGGGGCGGG AGGGGGCCGGGGCGGGGACCGGCCATGTCGGAGGTGACCCGGAGTCTGCTGCAGCGCTGGGGCGCCAGTC TTAGGAGAGGCGCCGACTTCGACTCTTGGGGCCAGCTGGTGGAGGCGATAGACGAGTATCAGATATTAGC AAGACACTTACAGAAGGAGGCCCAAGCTCAACACAATAATTCTGAATTTACAGAAGAACAAAAGAAAACC ATAGGCAAAATTGCGACATGTTTGGAATTGCGAAGTGCAGCATTACAGTCCACACAGTCCCAAGAAGAGT TTAAACTGGAGGACCTGAAGAAGCTAGAACCAATCCTGAAGAATATTCTTACATATAATAAAGAATTCCC ATTTGATGTTCAGCCTATTCCATTAAGACGTATTTTAGCACCTGGTGAAGAAGAGAATTTGGAATTTGAA GAAGATGAAGAAGGTGGTGCTGGAGCAGGGCCTCCTGATTCTTTCTCTGCTAGAGTTCCTGGTACTTTAT TGCCACGGTTGCCATCGGAACCAGGGATGACGTTACTCACCATCAGGATTGAGAAAATTGGTTTGAAAGA TGCGGGGCAGTGCATCGATCCCTACATCACAGTCAGCGTGAAAGATTTGAATGGCATAGATTTAACTCCC GTACAAGACACTCCTGTGGCTTCAAGGAAAGAAGATACTTACGTTCACTTTAATGTGGACATTGAGCTCC AGAAACACGTTGAAAGGTTAACCAAAGGTGCGGCCATTTTCTTTGAATTCAAACATTACAAGCCTAAAAA GAGGTTTACCAGCACTAAGTGTTTTGCCTTCATGGAGATGGATGAAATTAAACCTGGGCCAATCGTAATA GAACTATACAAGAAACCCACTGACTTTAAAAGAAAGAAATTGCAATTGTTGACCAAGAAACCACTTTATC TTCATCTACATCAAAGTTTGCACAAGGAATGATCCTGATACAAATAACCAAGAACTTGTGTGAATTTTAC CACTCAGAACCCATCATAGCTCTGAGTAGCATAATCACCCTTCAACAGGCAGGAAGTGAGCCCTTCAACA GGCAGGAAGTGAGCCCTACCCACGCCAGCTGACCAAGCGAGTCTATTGCACAGCCGGACCCCAGAACAAA AGTTCAGCACATCACTGACCTATCACTCCTTTGGATACAGGTTTATTGTAGATTTGGAAACGTTTTCACT TTTCTATTAATTGTGCAATGAATAGTCTATTTTCTCATTGCCTCTACTCCCCTGTTTCCCGGACAGCACT GTTGTAGATGTGCAGAACTTCAGACAATGCAGCAATAAGAATGTGCAAGAGTCTACGTCTCTGCTCACTT CTGCTCCAGTATGCTCTTCTGGTTTCAATGCCACGCTGTGAGTTGAAGTGGGTAGAACTGCTCTGAGAGG AACTGGACTAGTTACATTGCCTAGCAAAGTCTCCTTAGAAGAGACCTAGCCTGGTGCCATGGGTCTCCTG CATAGAAAGTGTGACCTGGCCAGATGAGCATCCCACCCTGAGTGTTCCCCGGATGACCAGACTGATTTCC TGAAGAGAGCACGTGTATCGTCAGGTGGATTATCTGCTCTTCTCTATTGTACTGCTAATTCTTTCTGGTA GTTCTAGGGTTAACGTTGGAACCATCTCAGAATAACTATGGATTTGAAAATGTGTTTACAGTATCTTTTA AACTGTATCATTTAGGAACAGTTGAGTCAGATGTCAAGATGTTGCGGGATAACTGCTGCTTTCCTGACCA CCCGTTGTGAGACCTTAAGACCCCGTACCTCTCCTTAGTATGCAAAAATCTGGAAAGACTGTCTATTTTT TTTTAATATAGGTAGATAGGATTGCCATTTATTTCCTATTTAGATATACAGTCATTCATCCATATGAAAA TATGCAGGTCACTAGTTTATTTTGAATGGACTATTTACTTTTGGTGTAACAAGGCATAAATAAAACTTTG AAAGCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5707830 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5707830 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007099 same experiment
	EMAGE:31758 same embryo
	EMAGE:29947 same embryo
	EMAGE:31671 same embryo
	EMAGE:31707 same embryo
	EMAGE:31678 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source