EMAGE mouse gene expression spatial database

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EMAGE:31402

Slc6a20 ( MGI:1336891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004446_01	euxassay_004446_02	euxassay_004446_03	euxassay_004446_04	euxassay_004446_05

euxassay_004446_06	euxassay_004446_07	euxassay_004446_08	euxassay_004446_09	euxassay_004446_10

euxassay_004446_11	euxassay_004446_12	euxassay_004446_13	euxassay_004446_14	euxassay_004446_15

euxassay_004446_16	euxassay_004446_17	euxassay_004446_18	euxassay_004446_19	euxassay_004446_20

euxassay_004446_21	euxassay_004446_22	euxassay_004446_23	euxassay_004446_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

midbrain meninges

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon meninges

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7580
Entity Detected:	Slc6a20, ( MGI:1336891)
Sequence:	sense strand is shown >T7580 GTCGAAAGCTGATTCCAGAGTCCTGGAAAGAAACAGTAGAAAACACAGGGGTTTGGGGGTGGGGCGCGGA AGAGGGCACAGGTGTTGCTCATTCTACAGTGAGAGTTGTCTCAGAAGATGGAATCACCTTCAGCACATGC GGTCTCCCTGCCAGAGGATGAGGAGCTCCAGCCCTGGGGAGGCGCTGGAGGACCTGGTCAGCACCCAGGA AGACCGAGGTCAACAGAGTGTGCCCACCCTGGGGTTGTGGAGAAGGTGCGGCCAAAGTGGGACAACCCCC TGCAGTTCCTACTTGTCTGTATCTCCTATGCTGTGGGCCTGGGCAATGTATGGCGCTTCCCCTACCTGTG CCAGATGTATGGCGGAGGGAGTTTCCTGGTCCCCTACATCATCATGCTCATTGTGGAGGGGATGCCACTC CTGTACCTGGAGCTGGCCGTGGGACAGCGCATGCGGCAGGGCAGCATTGGTGCCTGGAGGACCATCAGCC CCTACCTCAGTGGTGTCGGCATTGCTAGCTTGGTGGTCTCGTTTTTGGCCTCTGTGTACTTCAATGTCAT CAACACCTGGGCTCTTTGGTACCTCTTCCACTCCTTCCAGGATCCCCTGCCGTGGTCTGTCTGCCCACTG AATAGTAATCACACAGGCTACGATGAGGAGTGTGAGAAGGCTTCGTCGACACAGTACTTCTGGTACAGGA AAACACTCAACATCTCACCGTCCATCCAGGAGAATGGAGGAGTGCAGTGGGAGCCAGCGCTGTGCCTCAC CCTGGCCTGGCTGATGGTCTATCTGTGCATCCTGAGAGGCACCGAATCTACTGGCAAGGTGGTCTATTTC ACCACGTCACTGCCTTACTTTGTGCTCATCATCTACCTGGTCCGGGGCCTCACACTCCATGGAGCCACCA ATGGCCTGGCATACATGTTCACACCTAAGATTGAGCAGCTAGCCAACCCCAAGGCCTGGATCAATGCAGC CACGCAGATCTTCTTCTCACTGGGCTTAGGGTGTGGTGGCCTGATTGCTTTTGCCAGCTACAACGAACCC TCCAACGACTGCCAGAAGCATGCCCTCATTGTGTCTGTCATCAATAGTACTACTGCCATATTTTCCAGTA TTGTCACCTTCTCCATCTATGGCTTCAAGGCCACCTTCAACTATGAAAACTGCTTAAACAAGGTGATTCT GCTGCTGACCAATTCTTTTGACCTTGAAGATGGCTTTCTGACAGTCAGCAACCTGGAGGAGGTGAAGAAC TACCTGGCATCTACTTACCCAAACAAGTACAGTGAAGTGTTCCCACACATTAGAAACTGCAGCTTGGAAT CAGAGCTGGACACGGCTGTCCAGGGCACAGGCCTGGCCTTCATCGTCTACACTGAGGCCATTAAAAACAT GGAAGTTTCCCAGCTCTGGTCAGTGCTCTACTTCTTCATGTTGCTAACACTGGGTATGGGAAGCATGGTG GGGACAGGAACGGCCATCCTCACCCCTCTGACTGACAGCAAGATCATCTCCAGCTACCTGCCCAAGGAGG CCATTTCAGGTTTGGTATGCCTCCTCAACTGTGCCATTGGCATGGTGTTCACCATGGAGGCTGGGAACTA CTGGTTTGACCTATTCAATGACTATACAGCCACGCTGTCTCTGCTGCTCATTGTGCTGGTAGAGACCATA GCTGTGTGCTATGTGTATGGGCTAAAGAGATTTGAAAGTGACCTTCGGGCCATGACTGGCCGCACCCTCA GCTGGTACTGGAAGGTCATGTGGGCGTTTGTGAGTCCACTGCTCATCGTCGGCCTCTTTATCTTCTACCT GAGTGACTACATCCTCACGGGAACGCTGCAGTACCAAGCCTGGGATGCTACTCAGGGCCACGTGGTGACT AAGGATTATCCTACATACGCACTAGCTGTCATTGGGCTTCTGGTAGCCTCCTCCACCATGTGCATCCCTC TGGTAGCCCTGGGGACTTTTGTCACACGCCACTTCAAGATCAGAGAACAGTTTTCTGCAGCCTGAGAACT GGAGGTCCCACAGACTTCCTGTCAGTCACTGTTTCACAGCCACTCCCTATATATCCAGCAGTGGGAGGTC TACAGCTGCTTTTTAAAGCCACAAGTATTTTCTGCTTCCTGAATCTGCAAGGAAAATTAAATTCTCTGTA TTAAATTGTAGGCTGACAGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242168 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242168 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004446 same experiment
	EMAGE:31417 same embryo
	EMAGE:30372 same embryo
	EMAGE:30371 same embryo
	EMAGE:31481 same embryo
	EMAGE:31403 same embryo
	EMAGE:31457 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source