EMAGE mouse gene expression spatial database

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EMAGE:31403

Slc6a6 ( MGI:98488)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004447_01	euxassay_004447_02	euxassay_004447_03	euxassay_004447_04	euxassay_004447_05

euxassay_004447_06	euxassay_004447_07	euxassay_004447_08	euxassay_004447_09	euxassay_004447_10

euxassay_004447_11	euxassay_004447_12	euxassay_004447_13	euxassay_004447_14	euxassay_004447_15

euxassay_004447_16	euxassay_004447_17	euxassay_004447_18	euxassay_004447_19	euxassay_004447_20

euxassay_004447_21	euxassay_004447_22	euxassay_004447_23	euxassay_004447_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord mantle layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nose

strong

regional

strong expression: see section 17 moderate expression: see section 10 11 12 13 14 15 16 18 19

pons mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

lower jaw molar

moderate

regional

moderate expression: see section 07 08 09 19 20

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

strong

regional

strong expression: see section 17 moderate expression: see section 11 12

upper jaw incisor

strong

regional

strong expression: see section 17 moderate expression: see section 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7579
Entity Detected:	Slc6a6, ( MGI:98488)
Sequence:	sense strand is shown >T7579 CCCACGCGTCCGGGGAGAAGCCGCTTATAAATTACCGCTTCCTCCGCGCCGCCGCCAGCGTCGTGCTCCG GGACCTGGTTGCTGCCGAGCTCCCGTGCCCAGCCGCCAACGCCGCGATCGCCGCCAGTCCCGCCAGCCTC GCCAGCCCCGGGCCATCCGCTGTGGGCTTAGCCACCCAGGTGCAGAACCAGTGCCACAGCCTCTTCAGAG GAGCATCTCAAGCAAAACGAAGAGATGGCCACGAAGGAGAAGCTGCAATGTCTGAAAGACTTCCACAAAG ACATCCTGAAGCCTTCTCCAGGGAAGAGCCCAGGCACACGGCCTGAAGATGAGGCGGACGGGAAGCCCCC TCAGAGGGAGAAGTGGTCCAGCAAGATCGACTTTGTGCTGTCTGTGGCCGGAGGCTTCGTGGGTTTGGGC AACGTCTGGCGTTTCCCGTACCTCTGCTACAAAAATGGTGGAGGTGCGTTCCTCATACCGTATTTTATTT TCCTGTTTGGGAGCGGCCTGCCTGTGTTTTTCTTGGAGGTCATCATAGGCCAGTACACATCAGAAGGGGG CATCACCTGCTGGGAGAAGATCTGTCCTTTGTTCTCTGGCATTGGCTACGCATCCATCGTCATTGTGTCC CTCCTGAACGTGTACTACATCGTCATCCTGGCCTGGGCCACATACTACCTATTCCACTCTTTCCAGAAGG ATCTTCCCTGGGCCCACTGCAACCATAGCTGGAACACACCACAGTGCATGGAGGACACCCTGCGTAGGAA CGAGAGTCACTGGGTCTCCCTTAGCACTGCCAACTTCACCTCACCCGTCATCGAGTTCTGGGAGCGCAAT GTGCTCAGCCTGTCCTCCGGAATCGACAACCCAGGCAGTCTGAAATGGGACCTCGCGCTCTGCCTCCTCT TAGTCTGGCTCGTCTGTTTTTTCTGCATCTGGAAGGGTGTTCGATCCACAGGCAAGGTTGTCTACTTCAC CGCTACTTTCCCGTTTGCCATGCTTCTGGTGCTGCTGGTCCGTGGACTGACCCTGCCAGGTGCTGGTGAA GGCATCAAATTCTACCTGTACCCTGACATCAGCCGCCTTGGGGACCCACAGGTGTGGATCGACGCTGGAA CTCAGATATTCTTTTCCTACGCAATCTGCCTGGGGGCCATGACCTCACTGGGAAGCTATAACAAGTACAA GTATAACTCGTACAGGGACTGTATGCTGCTGGGATGCCTGAACAGTGGTACCAGTTTTGTGTCTGGCTTC GCAATTTTTTCCATCCTGGGCTTCATGGCACAAGAGCAAGGGGTGGACATTGCTGATGTGGCTGAGTCAG GTCCTGGCTTGGCCTTCATTGCCTACCCAAAAGCTGTAACCATGATGCCGCTGCCCACCTTTTGGTCTAT TCTGTTTTTCATTATGCTCCTCTTGCTTGGACTGGACAGCCAGTTTGTTGAAGTCGAAGGACAGATCACA TCCTTGGTTGATCTTTACCCGTCCTTCCTAAGGAAGGGTTATCGTCGGGAAATCTTCATAGCCATCTTGT GTAGCATCAGCTACCTGCTGGGGCTGACGATGGTGACGGAGGGTGGCATGTATGTGTTTCAACTCTTTGA CTACTATGCAGCTAGTGGTGTATGCCTTTTGTGGGTTGCATTCTTTGAATGTTTTGTTATTGCCTGGATA TATGGCGGTGATAACTTATATGACGGTATTGAGGACATGATTGGCTATCGGCCTGGGCCCTGGATGAAGT ACAGCTGGGCTGTCATCACTCCAGCTCTTTGTGTTGGATGTTTCGTCTTCTCGCTTGTCAAGTATGTACC CCTGACCTACAACAAAGTGTACCGGTACCCGGATTGGGCAATTGGGCTGGGCTGGGGCCTGGCCCTTTCC TCCATGCTGTGTATCCCCTTGGTCATTGTCATCCTCCTCTGCCGGACGGAGGGACCGCTCCGCGTGAGAA TCAAATACCTGATAACCCCCAGGGAGCCCAACCGCTGGGCTGTGGAGCGTGAAGGGGCCACACCCTTTCA CTCCCGAGTAACCCTCATGAACGGCGCACTCATGAAACCCAGTCACGTCATTGTGGAGACCATGATGTGA GGTCCGGGCCATGTGACAGGCGCCGCTTTCCTGCTGTTTACTAACGTTAGATTCTCATAGGACCAGGTTT ACAGAGCTTTATATTTGTACTAGGATTTTTTTTTTTAATTGTCACAGAAAATGTTACTCTATGTGTGTGT GTATGTGTATCGTGTATGTCTGTATATGTGTGTTTTGTTTTGTTTGGGGGATATTTTGTACAAAAAGAAA ACCCATAGGCCTACGTCCTGGGGAAGAGGATGGACTTTCATATTGATTTCCATGTATTTTGTGGGAACTT GGTAAATTTTTCTTTGTATTTTTTTTAACATATAACTATATATACTTAGAGTCTGTCATACACTTTGCCA CTTGAATTGGTCTTGCCAGCAATGGATCTCGTTTTTCAAAAGCAATTCTTCGGTGCTTATATAGCTGGCA GAAAGTTCTGCCCAAAAACAAATGAAAAAAGAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242193 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242193 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004447 same experiment
	EMAGE:31417 same embryo
	EMAGE:30372 same embryo
	EMAGE:30371 same embryo
	EMAGE:31481 same embryo
	EMAGE:31457 same embryo
	EMAGE:31402 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source