EMAGE mouse gene expression spatial database

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EMAGE:31418

Hdac4 ( MGI:3036234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006864_01	euxassay_006864_02	euxassay_006864_03	euxassay_006864_04	euxassay_006864_05

euxassay_006864_06	euxassay_006864_07	euxassay_006864_08	euxassay_006864_09	euxassay_006864_10

euxassay_006864_11	euxassay_006864_12	euxassay_006864_13	euxassay_006864_14	euxassay_006864_15

euxassay_006864_16	euxassay_006864_17	euxassay_006864_18	euxassay_006864_19	euxassay_006864_20

euxassay_006864_21	euxassay_006864_22	euxassay_006864_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 14 15 16

facial vii ganglion

strong

regional

strong expression: see section 01 02 moderate expression: see section 19

vagus x ganglion

moderate

regional

moderate expression: see section 06 16

thymus primordium

strong

regional

strong expression: see section 09 10 11 13 14 moderate expression: see section 12

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 06 16 17 18 19 20 21

vibrissa

strong

regional

strong expression: see section 02 03 04 05 06 07 19 20 moderate expression: see section 21

cervical ganglion

weak

regional

weak expression: see section 07 15

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 09 15

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 05 moderate expression: see section 16 17 18

thyroid gland

moderate

regional

moderate expression: see section 09 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9179
Entity Detected:	Hdac4, ( MGI:3036234)
Sequence:	sense strand is shown >T9179 CGGGCCTCCTCAATTTTGTTCCTGCATGTGCTGGTGGAAATTTGGAGTGTGTTGGAGCCACTGTTTACCT GGACATCTTGAGCCATTTTGAACTTAAGGCACTGACGCTGCTAGCAATGAGCTCCCAAAGCCATCCAGAT GGACTTTCTGGCCGAGACCAGCCTGTGGAGCTGCTGAATCCTGCCCGTGTGAACCACATGCCCAGCACGG TGGACGTGGCTACAGCGCTGCCTCTGCAAGTGGCCCCTACAGCAGTACCCATGGACCTGCGCTTGGACCA CCAGTTCTCACTGCCCTTGGAACCTGCATTGCGGGAGCAGCAACTGCAGCAGGAACTCCTAGCACTGAAA CAGAAGCAGCAGATCCAGCGGCAGATACTCATTGCAGAGTTCCAGCGTCAACATGAGCAGTTGTCCCGAC AGCATGAGGCACAGTTGCATGAACATATCAAGCAGCAGCAGGAGATGCTGGCCATGAAGCACCAGCAGGA GCTGCTGGAGCACCAGCGGAAACTGGAGCGGCACCGGCAAGAGCAGGAGCTGGAGAAGCAGCACCGTGAG CAGAAGCTGCAGCAGCTCAAGAACAAGGAGAAGGGCAAAGAGAGTGCTGTGGCGAGCACAGAGGTGAAGA TGAAGCTGCAGGAGTTTGTTCTCAACAAGAAGAAGGCTCTAGCCCACCGGAACCTGAACCACTGCATTTC CAGCGATCCCCGCTACTGGTATGGGAAGACACAGCACAGCTCCCTTGACCAGAGCTCTCCACCCCAGAGT GGGGTGTCAGCCTCCTACAACCACCCCGTCTTGGGAATGTACGACGCCAAAGATGACTTCCCTCTTAGGA AAACAGCTTCTGAACCTAACCTGAAATTACGCTCAAGGCTTAAGCAGAAAGTAGCTGAGAGACGGAGCAG CCCCCTGTTGCGCAGGAAAGATGGCCCTGTGGCCACTGCTCTAAAAAAGCGACCCCTGGATGTTACAGAC TCCGCATGCAGCAGCGCCCCTGGCTCCGGTCCCAGCTCTCCAAATAGCAGCTCTGGCAACGTCAGCACTG AGAATGGCATCGCACCCACTGTGCCCAGCGCTCCAGCTGAGACGAGCTTGGCACACAGACTTGTGACTCG AGAAGGCTCAGTCGCCCCACTTCCTCTCTACACGTCACCATCCTTACCCAACATCACCTTGGGACTTCCT GCCACTGGCCCTGCCGCTGGTGCGGCAGGTCAGCAGGATGCTGAGAGGCTTGCTCTCCCAGCTCTCCAGC AGCGGATCTTGTTCCCTGGGACCCACCTCACCCCGTACCTGAGCACCTCGCCCCTGGAGAGGGACGGTGC AGCAGCTCACAACCCCCTCCTGCAGCACATGGTCCTGCTGGAGCAGCCACCCACCCAGACACCCCTTGTC ACAGGCCTGGGGGCGCTGCCCCTCCACTCACAGTCCCTGGTTGGTGCGGACAGGGTGTCCCCATCCATTC ACAAGCTGCGGCAGCACCGCCCTCTGGGGCGCACGCAGTCAGCACCCCTGCCGCAGAACGCACAGGCCCT GCAGCACCTGGTGATCCAGCAGCAGCACCAGCAGTTCCTGGAGAAGCACAAGCAACAGTTCCAGCAGCAG CAGCTGCACCTCAGCAAGATAATCTCCAAACCTAGTGAGCCACCTCGGCAGCCTGAGAGCCACCCAGAGG AGACAGAGGAGGAGCTCCGTGAGCACCAGGCCTTGCTGGATGAGCCCTACCTAGATCGGCTACCTGGGCA GAAGGAGCCCTCCCTGGCTGGTGTGCAGGTGAAGCAGGAGCCCATTGAGAGTGAGGAGGAAGAAGCGGAG GCCACTCGAGAGACAGAGCCCGGCCAGCGCCCAGCCACTGAGCAGGAGCTGCTCTTCAGACAGCAAGCCC TCCTACTGGAGCAGCAGAGGATCCACCAGTTAAGAAACTACCAGGCATCTATGGAGGCTGCTGGCATCCC TGTGTCATTTGGCAGCCACAGACCTCTGTCTCGGGCACAGTCCTCCCCAGCATCTGCCACCTTCCCCATG TCAGTCCAGGAGCCCCCCACCAAGCCAAGGTTCACCACAGGTCTTGTGTATGACACACTGATGTTGAAGC ATCAGTGCACCTGTGGGAACACCAACAGCCACCCGGAGCATGCTGGGAGGATCCAGAGCATCTGGTCCCG CCTGCAGGAGACTGGACTCCGTGGCAAGTGTGAGTGCATCCGTGGACGCAAGGCCACATTGGAGGAGCTG CAGACAGTGCACTCGGAGGCCCACACACTCCTCTACGGCACAAATCCTCTCAACAGACAGAAACTGGACA GCTCGCTGACCTCAGTGTTCGTCAGGCTTCCTTGTGGTGGTGTTGGGGTGGATAGCGACACCATATGGAA TGAGGTGCACTCGTCTGGGGCAGCCCGCCTGGCTGTAGGCTGTGTAGTGGAGCTGGTCTTCAAGGTGGCC ACGGGAGAGCTAAAGAATGGCTTTGCTGTGGTTCGTCCCCCAGGACACCATGCCGAGGAGAGCACACCCA TGGGTTTCTGCTACTTTAACTCCGTGGCAGTTGCAGCCAAACTTCTCCAGCAGAGGCTGAATGTGAGCAA GATCCTCATTGTAGACTGGGATGTACATCATGGGAATGGGACCCAGCAGGCCTTCTACAATGACCCCAAT GTTCTCTACATGTCCCTGCACCGCTATGACGATGGGAACTTCTTCCCAGGAAGTGGAGCACCAGATGAGG TGGGCACAGGGCCAGGCGTGGGTTTCAATGTCAACATGGCTTTCACGGGTGGCCTCGAACCCCCCATGGG AGACGCTGAGTACTTGGCAGCCTTCAGAACGGTGGTTATGCCTATCGCAAATGAGTTTGCCCCAGATGTG GTACTGGTGTCATCGGGCTTCGATGCTGTGGAGGGCCACCCCACACCTCTTGGAGGGTACAATCTCTCTG CCAAATGTTTTGGGTACTTGACAAAACAGCTGATGGGCTTAGCTGGTGGCCGGCTTGTGCTGGCCCTTGA GGGAGGCCATGACCTGACAGCCATCTGTGATGCTTCTGAAGCCTGCGTGTCTGCTCTGCTGGGAAACGAG CTTGAGCCTCTGCCAGAAAAGGTTCTACATCAGAGACCCAATGCCAATGCTGTCCACTCCATGGAGAAAG TGATGGACATCCACAGCAAGTACTGGCGCTGCCTGCAGCGTCTGTCCTCCACGGTGGGGCACTCTCTGAT TGAGGCGCAAAAGTGTGAGAAGGAAGAAGCTGAGACAGTCACCGCCATGGCCTCGCTGTCTGTAGGCGTC AAACCTGCTGAGAAGAGATCTGAGGAGGAGCCCATGGAGGAGGAACCACCACTGTAGCCCGAAGCTGCTG TTCTCTCCTTTGTTTGTCTGTCTTGAAGCTCAGCCAAGAAACTTACCCGTATCACTCCTGCGTCCTGCCT TGGTGCTCTCTTGAGTACAAAGGGACAGCAGGCGTGCAGCAGCAACGGGAAGCCTTTCTGCCGCCCTGGA CCACAGGTCTGGAGACGCACACGGAACACCGGGTGTGGCAGATCACATGGAACACAGGACAGCGCGCAAC ACACGGGCACACAGACATGTGGAAGCCAAGCACACGCTGGTGGCTCCCCCTCCCCCTGGGAAGCCTCGAA GGAAGAAGCTTGTGGCAACTTGGGCAGAGTTGCTGAATCAAGTTGACATGAGGAAAATGAAAATCAAAGA TCTAATAATACAGAACAAACTTGATTAAAACTGGTGCTTAACATTTGATTCTTACCCACAATCCCACAGT CTCCGTGTAAACCACTCAACTCATCTTGTAGCTTTTTTTTTTTTTTAAACGAAAACAAAACCATTTTCTA TGGCTCTGGCCTGCCTTGTGAACCTTAGTGGGGTGCTGTGGGGGTTCACAGCTTAGTGAGGGACAGAGTT GGAAGAAAGTTTAAACGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827645 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827645 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006864 same experiment
	EMAGE:32001 same embryo
	EMAGE:31178 same embryo
	EMAGE:31448 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source