EMAGE mouse gene expression spatial database

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EMAGE:31480

Chrna2 ( MGI:87886)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006851_01	euxassay_006851_02	euxassay_006851_03	euxassay_006851_04	euxassay_006851_05

euxassay_006851_06	euxassay_006851_07	euxassay_006851_08	euxassay_006851_09	euxassay_006851_10

euxassay_006851_11	euxassay_006851_12	euxassay_006851_13	euxassay_006851_14	euxassay_006851_15

euxassay_006851_16	euxassay_006851_17	euxassay_006851_18	euxassay_006851_19	euxassay_006851_20

euxassay_006851_21	euxassay_006851_22	euxassay_006851_23	euxassay_006851_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

strong

regional

strong expression: see section 04 05 06 07 19 20 21 22 moderate expression: see section 08 18

ventral grey horn

strong

single cell

strong expression: see section 11 12 13 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7382
Entity Detected:	Chrna2, ( MGI:87886)
Sequence:	sense strand is shown >T7382 ATGATATGGCCTGGACTCAAAGTCACGCTTGCAGACTCTGGATGGGCTGCAGAGAGACAGGGCCTTGAAG AAGCCAGCTCCTTGTAGTCCAACAGAAACCAGGACCCTCTGAAGCCATGGCCCCCTCCCATCCTGCTTTC CAGTTCTGGATACATCTTTATCTCTGGTGTCTGCTTCTGATGCCAGCAGTGTTGGCCCAGCAAGGCTCGC ACACCCATGCCGAGGACCGCCTGTTCAAACACCTCTTTGGAGGCTACAATCGCTGGGCACGGCCAGTGCC CAACACTTCCGATGTGGTCATCGTGCGCTTTGGACTATCCATTGCACAGCTCATAGATGTGGATGAGAAG AATCAAATGATGACCACCAATGTCTGGCTAAAGCAGGAATGGAATGACTACAAGCTGCGCTGGGACCCGG CTGAGTTTGGCAACATCACCTCCCTCCGCGTCCCCTCAGAGATGATCTGGATCCCAGACATTGTTCTCTA CAACAATGCAGACGGGGAGTTTGCGGTGACCCACATGACCAAGGCCCACCTCTTCTTCACGGGCACTGTG CACTGGGTGCCCCCAGCCATCTACAAGAGCTCCTGCAGCATCGACGTGACTTTCTTCCCCTTCGACCAGC AGAACTGCAAGATGAAGTTTGGCTCCTGGACATACGACAAGGCCAAGATCGACCTGGAGCAGATGGAGCG GACGGTGGACCTGAAGGACTACTGGGAGAGCGGCGAGTGGGCCATTATCAACGCCACCGGAACCTATAAC AGCAAGAAGTACGACTGCTGCGCGGAGATCTACCCCGACGTCACCTACTACTTCGTTATCCGCCGGCTGC CGCTGTTCTACACCATCAACCTCATTATCCCGTGCCTGCTCATCTCCTGCCTCACCGTGCTCGTGTTCTA CTTGCCCTCTGAGTGTGGAGAGAAGATCACGCTGTGCATCTCGGTGCTGCTCTCCCTCACTGTCTTCCTG CTGCTCATCACAGAAATTATCCCATCCACCTCACTGGTCATCCCACTCATCGGCGAGTACCTGCTCTTCA CCATGATCTTCGTCACCCTCTCTATTGTGATCACAGTCTTCGTGCTCAATGTCCACCACCGTTCCCCTAG CACGCACAACATGCCCAACTGGGTGAGGGTGGCCCTGCTAGGCCGGGTGCCCCGGTGGCTGATGATGAAT CGGCCCCTGCCGCCCATGGAGCTCCATGGCTCTCCAGGTCTGAAGCTCAGCCCCACTTATCATTGGCTGG AGACCAACATGGATGCTGAAGAAAGGGAGGAGACAGAGGAAGAGGAGGAGGAGGAAGAAGATGAAAACAT ATGTATGTGTGCAGGCCTCCCAGACTCTTCCATGGGTGTCCTCTATGGCCATGGCAGCCTGCATCTGAGG GCCATGGGGCCTGAGGCCAAGACTCCATCCCAGGCTAGTGAGATCCTGCTGTCACCTCAAATACAGAAAG CACTAGAAGGTGTACACTACATTGCTGACCACCTGAGGTCTGAGGATGCTGACTCTTCGGTGAAGGAAGA TTGGAAGTATGTGGCCATGGTGGTAGACCGGATCTTCCTCTGGCTGTTCATTATCGTCTGCTTCCTGGGG ACCATCGGGCTCTTCCTCCCTCCGTTCCTGGCTGGAATGATCTGACTTGATGTCCCTCATGTTGGCTCCA AGGTGGCCTGGACAACCATCTTCTGCTCTTCTGTGACTGGAGCCATCTCTAGAATATTCTTTTGACTTAA TGCCACCTGCCTGCGATGTTTCTACGAGGCCCCTGATCTAGCCTGGACTTCATATCCTCCTTGTCAACAG TTCATGGTGCCATTGCGGTGACAAGCACTGAGACCACTCAACATGACAACTATTTAGGGATGCTGAGGAA CTAGAAGAGGTGAAGGCTGCTCTGTAGGTGATGACAACATGGTGGTGATATGGGGGGAGAGCAAGATAGT TCTGAAAGAGCATATAGGAGTTGTCTATGGCAAAAGGGTCAAACAAAGGGATGGAGGGGTCAGACCAGGA CAAAGGGAAATAGAACACTGGCCCAAAGATGGGCACAAGGGGACAATGGGAGTGCCTGGTGTGGAAGAGT TCTGCACAACACTGTGGATTCAGAATGTCAGAGAGGACAGCCAGCTGGGGTGGGGAGGCTGGGAGGCAAG TTGGGATATTTTTGACCCAGGCCCAAGAAGAAGCTCAGAGTCTGCACTGAACCTGTCTCTATGCACTTTG CAGGAAATGTCAGAGGGACAGGGGTACTTCCCAAAGGAGTGGGGCACAAAGAAAGTTCTAGAGCAGTGGC TTTCAACCTGTGTCGTGGGTTGCAACCCTTCAGGGAGTCAAACGACCCTTTCACAGGGGTCACATATCAG ATATCCTGCATATCAGATGTTTTACATGACAGTCCATAACAATATCAAAATTACTGTTATGAAGTTGAAA TGAAAACAATTTTGTGGTTGGGGTCACAACATGAGGAACTGTAAAGGGTCATAGCATTAGGAAGGTTGAG AACCACTGATCTAGGTGCCCTGGTGGATGCTTGAGGCAGAATAGCAACATTGGGTATAAGTTGCTGGGCC ATTATTCAACCAAAAGCACTTTGGAGCCTTCAAGTTCCCCTCAGTGTCATGTCTCACTGTTCTATGCTAC AAAAAAAGAGGTATAGCAATTTCTACAAGGGATCAGGGCTACCTTCAAATCAAGAGGACAAAGTAAGAAG GCAGTCTCCAATGTTGGCACCCTTCTTGCTGTCCACAGGGACCTGGTCCTCAATTCCTGGCTCTGTCAGC ATGCCTCACATAGGAACTGAGATCTTTGCATCAAGCTCTTTCAATTGTGTGCTCCTTCAGGGCAGAGTCT CTGCTCTTGTTGCTTTCACTCATCCTGAGCTGGGTGAGAGAGCTCAGTAGCTGGACCCTCCATCGTGAGT AGTGGAGAGAAACCAACCTGGTAGCTTTAGGCTCCAGAACTGCTACATAATACCAAAGCTTTGGGGGAGG ACTCTTCCCCAGTCTTCCCAGGGCCCCATCAGGTGACTAACCTGCTCTGAACTGTCCTGTGTAGCTTGTT CTTTTACTTGTAATATTCCACGACCCTTCATTGCATAGAAGCCCAGCGGATTGGGCCTTTCAGAACATTC TGCAGTCCCCAAGGAATTAGGTCCCAGACGCTAACAGCAATGAGATTCAGGGTCACTAAGAGATATAGAG ACCAAGTTTGTTCTCTGTCTTGGGGTGCTCTCCTTCCGAACCCCACACACAGCACCTCGACAGACCTAGG AGATCTGCTTGTCGGAGGCCTTACCCAGACTTCCTGTTACCAGTTCTAATGATACCCACTCAGTCTCAGG CCTTGGCTCCCCCTTTCTCCACTGAATGCAGCCGGCACCTCTTTTCTCTGTGTCAAACCCCAAGACATGA TTTCCTCATGCAGAGATGCTTGTTGGATGATCCCTACCCCCCATCCTCCTTGCATGTGAGTGAAATGTCA AAATAAAGCTAAAATGTCTGCCGTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4193582 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4193582 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006851 same experiment
	EMAGE:29506 same embryo
	EMAGE:31484 same embryo
	EMAGE:31366 same embryo
	EMAGE:31155 same embryo
	EMAGE:31159 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source