EMAGE mouse gene expression spatial database

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EMAGE:31545

Pdlim4 PDZ and LIM domain 4 ( MGI:1353470)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001915_01	euxassay_001915_02	euxassay_001915_03	euxassay_001915_04	euxassay_001915_05

euxassay_001915_06	euxassay_001915_07	euxassay_001915_08	euxassay_001915_09	euxassay_001915_10

euxassay_001915_11	euxassay_001915_12	euxassay_001915_13	euxassay_001915_14	euxassay_001915_15

euxassay_001915_16	euxassay_001915_17	euxassay_001915_18	euxassay_001915_19	euxassay_001915_20

euxassay_001915_21	euxassay_001915_22	euxassay_001915_23	euxassay_001915_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 12 13 15 16 17

ventral grey horn

strong

single cell

strong expression: see section 09 10 11 12

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15

stomach

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11

tongue

moderate

regional

moderate expression: see section 09 10 11 12 13 14

leg

strong

regional

strong expression: see section 04 05 06 07 08

pons ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 17 18

foot mesenchyme

strong

regional

strong expression: see section 01 02 03 04 08 09

body-wall mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 24

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 04 05 06 07 19 20 21

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

strong

regional

strong expression: see section 10 11 12

esophagus

weak

regional

weak expression: see section 10 11

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21

hand mesenchyme

strong

regional

strong expression: see section 01 02 03

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail mesenchyme

strong

regional

strong expression: see section 12 13 14 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

hindgut

weak

regional

weak expression: see section 13 14

hypothalamus ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3991
Entity Detected:	Pdlim4, PDZ and LIM domain 4 ( MGI:1353470)
Sequence:	sense strand is shown >T3991 AGCTCCGGCCCGCGATGACCCACTCGGTGACCCTGCGCGGCCCTTCACCCTGGGGCTTCCGCCTGGTGGG CGGCCGGGACTTCAGTGCTCCCCTCACCATCTCGCGGGTTCATGCTGGGAGCAAAGCGGCCCTGGCTGCC CTGTGCCCAGGTGACTTGATCCAAGCCATCAACGGCGAGAGCACTGAGCTCATGACACACCTGGAGGCCC AGAACCGCATTAAAGGCTGCCACGATCATCTTACGCTCTCGGTCAGCAGGCCTGAGAATAAGAACTGGCC CAGTGCTCCTGATGACAAGGCTCAAGCACATAGGATCCACATTGACCCTGAGTCCCAGGACTGCAGTCCA GCCACCAGCAGGCGCTCCTCAGTCTCTGGGATTAGTCTGGAGGACAACAGATCGGGCCTGGGATCTCCAT ATGGTCAGCCACCTCGCCTTCCAGTCCCTCACAATGGCAGCAGCAACGAGGCCACCCTGCCAGCCCAGAT GAGCGCTCTGCATGTGTCTCCACCCACCAGTGCCGACACAGCCAGGGTTCTCCCTCGGAACCGGGACTGC AGGGTAGACCTGGGTTCAGAGGTATACAGGATGTTAAGGGAGCCAGCAGAGCCCACAGCCTCGGAGCCGA AACAGTCAGGATCCTTCCGCTACCTGCAGGGCATGCTAGAGGCTGGCGAGGGCGGGGATCGGCCTGGGTC TGGTGGTCCCCGGAACCTCAAACCAGCAGCCAGCAAGCTCGGTGCTCCACTGAGTGGCCTGCAGGGGCTA CCAGAGTGCACGCGCTGCGGCCACGGGATCGTGGGAACCATTGTCAAGGCGAGAGACAAGCTCTACCATC CGGAGTGCTTCATGTGCAGCGACTGCGGCCTGAATCTCAAACAGCGTGGGTACTTCTTCCTAGATGAACG GCTGTACTGTGAGAATCACGCCAAGGCTCGAGTCAAGCCGCCGGAGGGATATGATGTGGTGGCTGTATAC CCCAATGCTAAGGTGGAACTTGTCTGAGCTGGAAAATCTTGTCTTGCCTCTGCTTCACTTAATGTTCCCT CATGGCCTGTGTAAATATATGTCCTCCTGGCCTTTCTAATAAAGTCCTTCTGCTTGCTCTGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5144166 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5144166 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001915 same experiment
	EMAGE:31292 same embryo
	EMAGE:31614 same embryo
	EMAGE:31682 same embryo
	EMAGE:29653 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source