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EMAGE:31564

Nfkbia nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha ( MGI:104741)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 19
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 19
thymus primordium
moderate moderate
single cellmoderate expression: see section 11 12 13 14 weak expression: see section 15
maxilla
moderate moderate
single cellmoderate expression: see section 05 06 17 18 20 21 22
naris
moderate moderate
regionalmoderate expression: see section 12 13 15 16
mandible
moderate moderate
single cellmoderate expression: see section 05 06 07 08 17 18 20 21 22
anterior naris
moderate moderate
regionalmoderate expression: see section 13
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36683
Entity Detected:Nfkbia, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha ( MGI:104741)
Sequence:sense strand is shown

>T36683
ACTCCATGAAGGACGAGGAGTACGAGCAAATGGTGAAGGAGCTGCGGGAGATCCGCCTGCAGCCGCAGGA
GGCGCCGCTGGCCGCCGAGCCCTGGAAGCAGCAGCTCACGGAGGACGGAGACTCGTTCCTGCACTTGGCA
ATCATCCACGAAGAGAAGCCGCTGACCATGGAAGTCATTGGTCAGGTGAAGGGAGACCTGGCCTTCCTCA
ACTTCCAGAACAACCTGCAGCAGACTCCACTCCACTTGGCTGTGATCACCAACCAGCCAGGAATTGCTGA
GGCACTTCTGAAAGCTGGCTGTGATCCTGAGCTCCGAGACTTTCGAGGAAATACCCCTCTACATCTTGCC
TGTGAGCAGGGCTGCCTGGCCAGTGTAGCAGTCTTGACGCAGACCTGCACACCCCAGCATCTCCACTCCG
TCCTGCAGGCCACCAACTACAATGGCCACACGTGTCTGCACCTAGCCTCTACTCACGGCTACCTGGCCAT
CGTGGAGCACTTGGTGACTTTGGGTGCTGATGTCAACGCTCAGGAGCCCTGCAATGGCCGGACAGCCCTC
CACCTTGCGGTGGACCTGCAGAATCCTGACCTGGTTTCGCTCTTGTTGAAATGTGGGGCTGATGTCAACA
GGGTAACCTACCAAGGCTACTCCCCCTACCAGCTTACCTGGGGCCGCCCAAGTACCCGGATACAGCAGCA
GCTGGGCCAGCTGACCCTGGAAAATCTCCAGATGCTACCCGAGAGCGAGGATGAGGAGAGCTATGACACG
GAGTCAGAATTCACAGAGGATGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90266. Forward Primer - name:090266_F_cDNA_Nfkbia, sequence:ACTCCATGAAGGACGAGGAGTA; Reverse Primer - name:090266_N_SP6_cDNA_Nfkbia, sequence:TCATCCTCTGTGAATTCTGACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009409 same experiment
 EMAGE:31071 same embryo
 EMAGE:32033 same embryo
 EMAGE:32034 same embryo
 EMAGE:30141 same embryo
 EMAGE:30453 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS