EMAGE mouse gene expression spatial database

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EMAGE:31593

Neurog1 neurogenin 1 ( MGI:107754)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006581_01	euxassay_006581_02	euxassay_006581_03	euxassay_006581_04	euxassay_006581_05

euxassay_006581_06	euxassay_006581_07	euxassay_006581_08	euxassay_006581_09	euxassay_006581_10

euxassay_006581_11	euxassay_006581_12	euxassay_006581_13	euxassay_006581_14	euxassay_006581_15

euxassay_006581_16	euxassay_006581_17	euxassay_006581_18	euxassay_006581_19	euxassay_006581_20

euxassay_006581_21	euxassay_006581_22	euxassay_006581_23	euxassay_006581_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 moderate expression: see section 05 09 10 11 12 13 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 13 14 15 16 17 18

diencephalon roof plate

moderate

regional

moderate expression: see section 10 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7976
Entity Detected:	Neurog1, neurogenin 1 ( MGI:107754)
Sequence:	sense strand is shown >T7976 CAGGAGCTGATCTGATCGCCGGCGACATCACTCAGGAGACCAGCCCGGCGCGTGGCCCCTGCAGGCGAGG CGAGGAGGGCCAAGCCCATTCACTCCCTGAGCCCCTGCGATCTTCCCGGCCCTCGCGCCTGCAGCAGGCA CAGGCTAGCCCCGGGTCGTACGGACAGTAAGTGCGCTTCGAAGGCCGACCTCCAAACCTCCTGTCCGTCT GTCGGTCCTGCACACTGCAAGATGCCTGCCCCTTTGGAGACCTGCATCTCTGATCTCGACTGCTCCAGCA GCAACAGCAGCAGCGACCTGTCCAGCTTCCTCACCGACGAGGAGGACTGTGCCAGGCTACAGCCCCTAGC CTCCACCTCGGGGCTGTCCGTGCCAGCCCGGAGGAGCGCTCCCGCCCTCTCCGGGGCATCGAATGTTCCC GGTGCCCAGGACGAAGAGCAGGAACGGCGGAGGCGGCGAGGTCGCGCTCGGGTGCGGTCCGAGGCTCTGC TGCACTCCCTGCGGAGGAGTCGTCGCGTCAAAGCCAACGATCGCGAGCGCAACCGCATGCACAACCTCAA CGCTGCGCTGGACGCCTTGCGCAGCGTGCTGCCCTCGTTCCCCGACGACACCAAGCTCACCAAGATTGAG ACGCTGCGCTTCGCCTACAACTACATCTGGGCCCTGGCTGAGACACTGCGCCTGGCAGATCAAGGGCTCC CCGGGGGCAGTGCCCGGGAGCGCCTCCTGCCTCCGCAGTGTGTCCCCTGTCTGCCCGGGCCCCCGAGCCC GGCCAGCGACACTGAGTCCTGGGGTTCCGGGGCCGCTGCCTCCCCCTGCGCCACTGTGGCATCACCACTC TCTGACCCCAGTAGTCCCTCGGCTTCAGAAGACTTCACCTATGGCCCGGGCGATCCCCTTTTCTCCTTTC CTGGCCTGCCCAAAGACCTGCTCCACACGACGCCCTGTTTCATCCCATACCACTAGGCCTTTGTAAGGCA ACATCAATACTTTCTTCCTCCCCCAGTCTAAGAGCAATAATAGATGGGGAACTGGCTGAAGCCTCCGGGG GCCACACTTACCCCCAAGTGAATTCTGGGAGCTTTAAAGGGGGGAGGGGGAATACCTGACCACTTGTTAG GTTGCTGCACCCTCGCTGAAGCTGCCCTCGGTCTATTTCTCCACCCCCAGCACGGCCTCCCCCCCCCCCC GCCCGCCCCCAGACGGCCTTTCGTTTTTGTTGCACTTTCTGAACTTCACAAAACCTTCTTTGTGACTGGC TCAGAACTGACCCCAGCCACCACTTCAGTGTGGTTTGGAAAAGGGACAGATGAGCCCCTGAAGACGAGGT GAAAAGTCAATTTTACAATTTGTAGAACTCTAATGAAGAAAAACGAGCATGAAAATTCGGTTTGAGCCGG CTGACAATACAATGGCAAGGCTTAAAAAGGAGCCACAAGGAGTGGGCTTCATGCATTATGGATCCCGACC CCCGCCACTGTGGGCTTGCTCCAGGAAGAACCTGAGTGCTAGCATAGCTATTCAGGCACTGGACTGGAGG GTACTTTAATTTATTCAGGATGCTTCATTCATATGAAAATGTATTTTTGTACATAAAGAATTTATTCTAT TATTATGAGCTATCAAAGTTTACATTTTTGTACTGCAGATGCTTCGTGTAAATAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30146192 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30146192 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006581 same experiment
	EMAGE:30384 same embryo
	EMAGE:30382 same embryo
	EMAGE:30035 same embryo
	EMAGE:31327 same embryo
	EMAGE:30941 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source