EMAGE mouse gene expression spatial database

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EMAGE:31602

Btk ( MGI:88216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005012_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 08 09 10 11 12 moderate expression: see section 07

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10

liver right lobe

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8064
Entity Detected:	Btk, ( MGI:88216)
Sequence:	sense strand is shown >T8064 AAGAGGTAATCCAGTCCTGGCATCTCACGACGTCTGGGGAGCTACCTGCATTAAGTCAGAACTGAGTACA CAAACAAGTTCCAGAGAGAGGAAGCCATGGCTGCAGTGATACTGGAGAGCATCTTTCTGAAGCGCTCCCA GCAGAAAAAGAAAACATCACCTTTAAACTTCAAGAAGCGCCTGTTTCTCTTGACTGTACACAAACTTTCA TACTATGAATATGACTTTGAACGTGGGAGAAGAGGCAGTAAGAAAGGTTCAATAGATGTTGAGAAGATCA CCTGTGTTGAAACAGTAATTCCTGAAAAAAATCCCCCACCAGAAAGACAGATTCCGAGGAGAGGTGAGGA GTCTAGTGAAATGGAACAGATTTCAATCATTGAAAGGTTCCCGTACCCATTCCAGGTTGTATATGATGAA GGACCTCTCTATGTTTTCTCCCCAACTGAAGAGCTGAGAAAGCGCTGGATTCACCAGCTCAAAAATGTAA TCCGGTGCAATAGTGACCTGGTACAGAAATACCATCCTTGCTTCTGGATTGATGGACAGTATCTCTGCTG CTCTCAGACAGCCAAGAATGCTATGGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGG AGTTCTCATCGAAAAACGAAAAAGCCTCTTCCCCCTACCCCAGAGGAAGATCAGATCTTGAAAAAACCGC TTCCCCCGGAGCCAACAGCAGCACCAATCTCCACAACCGAGCTGAAAAAGGTCGTGGCCCTTTATGATTA CATGCCAATGAACGCAAATGACTTACAATTGCGAAAGGGCGAGGAGTATTTTATCCTGGAGGAGAGCAAC CTACCGTGGTGGCGAGCACGAGATAAAAATGGGCAGGAAGGCTACATCCCAAGTAACTATATCACTGAAG CTGAGGACTCCATAGAGATGTATGAGTGGTATTCCAAGCACATGACTCGAAGTCAAGCTGAGCAACTGCT AAAGCAAGAGGGGAAAGAAGGAGGTTTCATTGTCGGAGACTCCAGCAAAGCTGGAAAATACACCGTGTCT GTGTTTGCTAAATCTACTGGGGAGCCTCAAGGGGTGATCCGCCATTACGTTGTGTGTTCCACGCCACAGA GCCAGTATTACCTGGCTGAGAAACACCTCTTCAGCACCATCCCTGAGCTCATTAACTACCATCAACACAA CTCTGCAGGCCTCATATCCAGGCTGAAATATCCTGTGTCTAAACAAAACAAAAACGCGCCTTCTACTGCA GGCCTGGGCTATGGATCATGGGAAATTGATCCAAAGGACCTCACCTTCTTGAAGGAGCTTGGGACTGGAC AATTCGGTGTCGTGAAATATGGGAAGTGGAGGGGCCAATATGATGTGGCCATCAAGATGATCAGAGAAGG TTCCATGTCGGAGGATGAATTCATTGAAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTGGTG CAGTTGTATGGCGTCTGCACCAAACAACGCCCCATCTTCATCATCACCGAGTACATGGCTAATGGCTGCC TCTTGAACTACCTGAGGGAGATGCGGCACCGCTTCCAGACACAGCAGCTGCTTGAGATGTGCAAAGATGT CTGTGAAGCAATGGAATACTTGGAGTCGAAGCAGTTCCTTCACAGAGACCTGGCAGCTCGAAACTGTTTG GTAAACGATCAAGGAGTTGTGAAAGTATCTGACTTTGGCCTGTCTAGGTATGTCCTTGATGATGAGTACA CCAGCTCTGTAGGCTCCAAGTTTCCAGTCCGGTGGTCTCCACCAGAAGTGCTTATGTATAGCAAGTTCAG CAGCAAATCTGACATCTGGGCTTTTGGGGTTTTAATGTGGGAGATCTACTCCCTGGGGAAGATGCCGTAT GAGAGATTTACTAACAGTGAGGCAGCAGAACACATTGCTCAAGGCTTACGTCTCTACAGGCCTCATCTGG CATCAGAGAGGGTATATACCATCATGTACAGCTGCTGGCACGAGAAAGCAGATGAACGTCCTAGTTTCAA AATTCTCTTGAGTAACATTCTAGATGTGATGGATGAAGAATCCTGAGCTGGCTGCTAAGCTCCGTGGATC TCCTCCTCTCTCCTACAAAACCTAATTCCATGTTTCCTGAGGAGTTCCCTGGCTGCAGGGCTCTAGCCTT CCATGCGCCTACTGAATGCATGAAGAGCCCTGGACATCTAGGAATGCCTTTCTCCTCTCGTTCCCTGGCA TACTGCTCTAAGCAAAGGTCAAGGGATTTCTGTGCATAGTATTACCCATAACTTCAAGACTCCTAACAGA CTGAATTGGGGATGGGAACACTTTGGGGGAGGGAAAACTGTAAATAGCTCCACTAGTTGTCCAACAGCTT GTTGGTTAAGTGTTAAGAGTGGTGGTGGTGGTGGGGGGGTAGGAATGTTGCCATTAAAATGGGTTGTTTT GTTACAATAAAATTATTGGAAAGAGTAAGAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30075322 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30075322 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005012 same experiment
	EMAGE:31550 same embryo
	EMAGE:30641 same embryo
	EMAGE:29954 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source