EMAGE mouse gene expression spatial database

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EMAGE:31666

Trappc3 ( MGI:1351486)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004809_01	euxassay_004809_02	euxassay_004809_03	euxassay_004809_04	euxassay_004809_05

euxassay_004809_06	euxassay_004809_07	euxassay_004809_08	euxassay_004809_09	euxassay_004809_10

euxassay_004809_11	euxassay_004809_12	euxassay_004809_13	euxassay_004809_14	euxassay_004809_15

euxassay_004809_16	euxassay_004809_17	euxassay_004809_18	euxassay_004809_19	euxassay_004809_20

euxassay_004809_21	euxassay_004809_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 10 11 weak expression: see section 12

vagus x ganglion

moderate

regional

moderate expression: see section 05 13

thymus primordium

moderate

homogeneous

moderate expression: see section 07 08 09 10 11

trigeminal v ganglion

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 06 14 15 16 17 18

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 14 15

spinal cord

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6067
Entity Detected:	Trappc3, ( MGI:1351486)
Sequence:	sense strand is shown >T6067 GGGGCCGCGGCTGGGTTGAATCGGCGACTCCCGGGGTCTGGGATCTGGGTTGTGAGGAGGTGAGGTGAGG CGGCACTGAACGGTGGGCACCATGTCGAGGCAGGCGAACCGTGGCACGGAGAGCAAGAAAATGAGTTCTG AGCTGTTCACGCTGACCTATGGAGCGCTTGTCACCCAGCTGTGCAAGGACTATGAAAACGACGAAGACGT GAACAAGCAGCTGGACAGAATGGGCTACAACATTGGAGTCCGACTGATTGAAGATTTTTTGGCACGGTCA AATGTTGGAAGATGTCATGACTTTCGGGAAACTGCAGATGTTATTGCTAAGGTGGCGTTCAAGATGTACC TGGGCATCACTCCAAGCATCACCAACTGGAGCCCAGCCGGAGATGAATTCTCCCTCATCCTGGAAAACAA CCCTCTGGTGGACTTTGTGGAGCTTCCCGATAACCACTCGGCCCTCATTTACTCCAACCTCCTGTGTGGG GTGTTGCGGGGAGCCCTGGAGATGGTCCAGATGGCTGTGGAGGCCAAGTTTGTCCAGGACACTCTGAAGG GAGATGGCGTGACAGAAATCAGGATGAGGTTCATCAGGCGGATTGAAGACAACCTCCCAGCTGGAGAAGA GTAAGCATCTCTAGGACTTGGGCAGCCAAGAGGATGCCTGCTGGGATGAGAGAGGCAGGCGCTCGCTTCC TCTGCCCCTGGAACTCAGTGACTTTTAAACTGATGTTATATATTCTTCTAACCTTATTTCCATCCTCCAT GCTAATAGAGAGCAGACTGATAGTCTGTTTGCCCCACAAATATGCACCTCAGAAGGGGGTTCTTTGCTCC ATTTCCCTTCAGAAGGGCAGGATGTACTTGCCCTTGGTTGGACTAAGAGAGCTCAAACCATTTTACATTC CTGTGAATTTTCCAAAGCAAAGCCCACTTTGACCCCATTAAGGGACGAGCCTGATGCATCTGTTCCTGCG ACTTCATGACGCCATTGGCAAATGGCTGTAGGGAATCTTGGGTTGTGGAGGGGAGGGAGAACGCTGGGAA GGGGTGGGTAAGAATTGTTGGCTGTAGTGACACTGTAGTCTGCCAGGCCACGGAAGCCAGTCCTGCCGGA AACACCGGCTTCTGGGAAGCCGCCCAGGTCTCATTCCTCCCTGCTGTTTGGAGGCAACATCTCCTCTTTT TACGGAGGGTCCATCCTTTTTTCTTACAAATTCTTCAATAAAGACACATTCTTGAGTGAAGCCCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3490979 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490979 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004809 same experiment
	EMAGE:32093 same embryo
	EMAGE:31196 same embryo
	EMAGE:30212 same embryo
	EMAGE:29862 same embryo
	EMAGE:32114 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source