EMAGE mouse gene expression spatial database

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EMAGE:31678

Gckr ( MGI:1096345)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007079_01	euxassay_007079_02	euxassay_007079_03	euxassay_007079_04	euxassay_007079_05

euxassay_007079_06	euxassay_007079_07	euxassay_007079_08	euxassay_007079_09	euxassay_007079_10

euxassay_007079_11	euxassay_007079_12	euxassay_007079_13	euxassay_007079_14	euxassay_007079_15

euxassay_007079_16	euxassay_007079_17	euxassay_007079_18	euxassay_007079_19	euxassay_007079_20

euxassay_007079_21	euxassay_007079_22	euxassay_007079_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain mantle layer

moderate

single cell

moderate expression: see section 09 10 12 weak expression: see section 08 11

liver left lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

strong

single cell

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 09 10 12 weak expression: see section 07 08 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7220
Entity Detected:	Gckr, ( MGI:1096345)
Sequence:	sense strand is shown >T7220 GCAGAGGCAGGCGGGTTGGAGAGTCTGCAGTGTGGGACCATGCCAAGCACCAAGCGGTATCAGCATGTGA TCGAGACCCCTGAGCCTGGGGAATGGGAGTTGTCAGGGTATGAAGCAGCTGTGCCAATCACAGAGAAGTC CAACCCACTGACCCGGAACTTGGACAAAGCAGATGCAGAGAAAATTGTTCAACTGCTGGGGCAGTGTGAT GCTGAGATATTCCAGGAGGAGGGGCAAATCATGCCCACCTACCAGCGACTGTACAGTGAGTCAGTTCTGA CCACCATGTTGCAAGTGGCTGGCAAGGTCCAGGAAGTGCTGAAGGAGCCAGATGGGGGCCTGGTGGTGCT GAGTGGAGGGGGCACCTCTGGTCGTATGGCATTCCTTATGTCTGTGTCTTTCAACCAGCTGATGAAAGGT CTGGGACAAAAACCTCTTTACACATACCTCATTGCAGGGGGTGACAGGTCTGTTGTAGCCTCTCGGGAAC GGACAGAAGATAGCGCCCTACACGGAATCGAGGAGCTGAAGAAGGTGGCTGCTGGGAAAAAGAGAGTGGT CGTTATAGGCATTTCCGTGGGACTCTCTGCGCCCTTTGTGGCAGGCCAGATGGACTACTGCATGGATAAC ACAGCTGTCTTCTTGCCGGTCCTGGTTGGCTTCAATCCGGTGAGCATGGCCAGAAATGATCCCATTGAAG ACTGGAGATCGACATTCCGACAAGTGGCAGAGCGGATGCAGAAGATGCAGGAGAAACAGGAAGCCTTTGT GCTCAATCCTGCCATCGGGCCTGAGGGGCTCAGTGGCTCTTCCCGAATGAAAGGTGGAAGCGCCACCAAG ATTCTACTGGAAACCCTGCTACTAGCAGCCCATAAGACTGTGGACCAGGGTGTTGTGTCCTCTCAAAGAT GCCTTCTGGAAATCCTGAGGACATTTGAGCGGGCTCATCAGGTAACCTACAGTCAAAGTTCCAAAATTGC CACTCTGACGAAGCAAGTTGGCATCAGCCTGGAGAAAAAAGGCCACGTGCACTTGGTTGGCTGGCAGACC CTCGGTATCATCGCCATTATGGATGGGGTAGAGTGTATCCACACTTTTGGTGCTGATTTCCGAGATATCC GTGGCTTTCTTATTGGTGACCACAATGACATGTTTAACCAGAAGGATGAGCTCAGCAATCAGGGTCCCCA GTTCACCTTCTCTCAGGATGACTTCCTGACTTCTGTTCTGCCATCCCTTACGGAAATTGACACTGTGGTC TTCATTTTTACCCTGGATGATAACCTCGCAGAAGTACAGGCCCTGGCAGAAAGGGTGAGGGAGAAGAGTT GGAACATCCAGGCCCTGGTGCACAGCACAGTGGGGCAGTCCTTGCCAGCTCCTCTAAAGAAGCTCTTTCC CTCGCTCATCAGCATCACATGGCCACTTCTTTTCTTCGATTATGAAGGGAGCTACGTTCAGAAGTTCCAG CGTGAGTTAAGCACCAAGTGGGTGTTGAATACAAGGTTCTCAGGACAGTCCAAGGCTCGCTGCATTGAGA GTCTTCTTCAAGTGATACATTTCCCTCAACCGCTGTCGAATGATGTCCGCGCGGCCCCCATCTCCTGCCA TGTCCAGGTTGCCCACGAGAAGGAAAAGGTGATCCCCACAGCCTTGCTGAGTCTCCTACTCAGGTGCTCC ATCACTGAGGCTAAGGAACGCCTGGCTGCAGCTTCTTCAGTCTGTGAGGTTGTTAGGAGCGCCCTCTCTG GGCCAGGTCAGAAACGCAGCATCCAAGCCTTTGGAGACCCTGTGGTGCCCTGAATTGATGTTTCTGTGAC CATTGAAAAGTCTCCATCCACTTCTGAGGGGACTTGTGCCAGTAATACACGTTGCAGGACAGACTCAGTT CCGGTGGGTGGGGCACTAACAGCCCAGAATTGAGGAAGAGTCCTGTTCTCAGCTGGATCATTTCCATTTT TACTGGCATCTTCTGATCTTAGGAATAAAACTAAATGTCTTGTTTTGAACCCAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4159892 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4159892 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007079 same experiment
	EMAGE:31758 same embryo
	EMAGE:29947 same embryo
	EMAGE:31671 same embryo
	EMAGE:31285 same embryo
	EMAGE:31707 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source