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EMAGE:31707

Melk maternal embryonic leucine zipper kinase ( MGI:106924)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707
euxassay_007082_01 euxassay_007082_02 euxassay_007082_03 euxassay_007082_04 euxassay_007082_05
EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707
euxassay_007082_06 euxassay_007082_07 euxassay_007082_08 euxassay_007082_09 euxassay_007082_10
EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707
euxassay_007082_11 euxassay_007082_12 euxassay_007082_13 euxassay_007082_14 euxassay_007082_15
EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707
euxassay_007082_16 euxassay_007082_17 euxassay_007082_18 euxassay_007082_19 euxassay_007082_20
EMAGE:31707 EMAGE:31707 EMAGE:31707 EMAGE:31707
euxassay_007082_21 euxassay_007082_22 euxassay_007082_23 euxassay_007082_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
esophagus lumen
moderate moderate
regionalmoderate expression: see section 10 11
metanephros
moderate moderate
regionalmoderate expression: see section 06 07 08 09 17 18 19 weak expression: see section 16
clavicle
moderate moderate
regionalmoderate expression: see section 08 09 15 16
rectum
strong strong
regionalstrong expression: see section 13
testis
weak weak
regionalweak expression: see section 05 06 07 18 19
midgut loop
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16
bladder
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 12
pancreas
strong strong
regionalstrong expression: see section 09 10 11 12 15 moderate expression: see section 16
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 19 20
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 19
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07 16
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 19
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23 24
thymus primordium
strong strong
regionalstrong expression: see section 10 11 12 13 14
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 17 18 19 20 21
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 12 13 15 16
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 08 21 22 23 weak expression: see section 04 24
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 weak expression: see section 21 22
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 14 15 16 17 18 19
hindgut
strong strong
regionalstrong expression: see section 13 14 15
naris
moderate moderate
regionalmoderate expression: see section 13 15 16 17
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 13 14 15 16 17 18
4th ventricle
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 20 21 22 23 24
mandible
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 15 16 17 18 19 20 21 22
ventral grey horn
strong strong
regionalstrong expression: see section 08 09 10 11 13 14 moderate expression: see section 12
liver right lobe
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10
urethra of male
moderate moderate
regionalmoderate expression: see section 12 13 14
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 06 17
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 16
midgut
strong strong
regionalstrong expression: see section 17 18
urinary system
strong strong
regionalstrong expression: see section 04 20 21
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 14 15 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 10 11 14 15
submandibular gland primordium
strong strong
regionalstrong expression: see section 08 09 10 15 16 17
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 12 15
maxilla
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 18 19 20 21
adrenal cortex
weak weak
regionalweak expression: see section 07 08 09 15 16 17
liver left lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11
lower jaw incisor
strong strong
regionalstrong expression: see section 11 moderate expression: see section 12 14 15 16
upper jaw incisor
strong strong
regionalstrong expression: see section 11 moderate expression: see section 12 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7661
Entity Detected:Melk, maternal embryonic leucine zipper kinase ( MGI:106924)
Sequence:sense strand is shown

>T7661
AAGAGGTAATCCATCTATGTCGGGTGGTCGACAAGAGGTAATCCCTTTGGCGGGTGGAAGCTGCTGGAAC
TTGAATCCTGCAGAAATTCCCACCGACTTGTTCCGGCTTTGCTAGACTCTGCAGCCGGCTGCTTCACCTT
CTGTCCTGTTCGGTCCTGTTCGGTCCTTGCTGGGCCCGCTGCCCTTCAGGTTCTGGTTGTCATTTTGAAT
AACCTTCCAGGTGGACTATGAAAGATTATGACGAACTCCTTAAATACTATGAACTATATGAAACGATTGG
GACAGGTGGCTTTGCAAAGGTCAAACTGGCCTGCCATGTCCTCACTGGAGAGATGGTAGCTATAAAAATC
ATGGATAAGAATGCGCTAGGGAGTGATTTGCCCCGAGTCAAAACTGAGATCGATGCGCTGAAGAGTCTGA
GACATCAGCACATATGTCAGCTCTACCATGTGCTGGAGACAAAGAACAAAATATTCATGGTTCTGGAGTA
CTGTCCAGGAGGAGAGCTGTTTGACTACATAATCTCCCAGGATCGCCTGTCGGAAGAGGAGACCCGGGTC
GTCTTCCGTCAGATACTGTCTGCAGTTGCGTATGTCCACAGCCAGGGCTATGCCCACAGGGACCTCAAAC
CAGAAAATTTATTATTTGATGAAAATCATAAGCTAAAGCTGATTGACTTTGGTCTTTGTGCAAAACCCAA
GGGCAACAAGGACTACCATCTGCAGACGTGCTGTGGGAGCCTTGCTTATGCAGCTCCTGAACTAATACAA
GGGAAGTCGTACCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATCCTCCTGTATGTGCTCATGTGTG
GATTTCTACCATTTGATGATGATAATGTCATGGCTTTGTACAAGAAGATAATGAGAGGGAAATACGAAGT
TCCTAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTCCAGCAGATGTTGCAGGTGGACCCAAAGAAACGG
ATTTCTATGAGAAATCTCCTGAACCATCCCTGGGTCATGCAAGATTACAGCTGTCCCGTGGAGTGGCAAA
GCAAGACTCCTTTGACTCACCTCGATGAGGATTGCGTGACAGAGCTTTCTGTACATCACCGCAGCAGCAG
GCAGACAATGGAGGATTTAATTTCGTCGTGGCGGTACGATCACCTCACAGCCACCTACCTTCTGCTTCTA
GCCAAGAAGGCCCGGGGGAAGCCGGCTCGTCTACAGCTCCTGTCCTTCTCTTGTGGAACCGCCAGCACCA
CTCCAAAGTCAAAGAATCTGAGCCTGGAAGATATGAGCACAAGTGATGATAACTGTGTGGCTGGATTGAT
AGACTATGAATTGTGTGAAGATAAATTATTAGCTCCCAAGACGCCACAGGTTACCAAACACTTGGCAGAA
TCAAATCACGCAGCATCTAAATCACCAGCGCCAGGGGTACGCAGAGCAGTGGCAAATAAATTAATGGACA
AAGAAAATGTGTGCACTCCCAAGTCTTCTGTGAAGAATGAAGAGCAGTTTGTATTTTCTGAGCCGAAGAT
TCCAGTTAGTAAGAACCAGTATAAGAGAGAAATACCCGCCTCACCAACCCGTTTCCCAACACCTGCAAAA
GCTAGAGCCCAGTGCCTGAGAGAAGCCCCGGTTAGAACACCAGGGAATTCCGCAGGAGCAGACACACTAA
CGACAGGTGTCATTAGCCCCGAGAGGAGGTGCCGTTCAATGGACGTGGATCTCAACCAGGCACACATGGA
GGATACCCCGAAAAAGAAAGGAACCAATGTGTTTGGGAGCCTTGAGAGAGGACTGGATAAGGTTCTCACT
GCGCTCACAAGGAACAAGAAGAAGGGCTCTGCCAGAGATGGACCAAGAAAGCGAAAGCTGCACTACAATG
TGACTACAACTCGCCTGGTGAACCCCGACCAGCTCCTGAGCGAAATCATGGCTATTCTTCCAAAGAAGAA
CGTGGACTTCGTACAGAAAGGTTACACTCTAAAGTGTCAAACGCAGTCTGATTTTGGCAAAGTGACAATG
CAGTTTGAACTGGAAGTGTGCCAGCTGCAGAGACCTGACGTGGTAGGCATCCGGAGACAGCGGCTGAAGG
GTGATGCCTGGGTTTACAAGAGATTAGTGGAAGATATCTTGTCTGGCTGCAAGATGTGACCGATGACAGT
GTCCGTCTGCCCGGATGAATGTGGGTGTGATGTTGCACACAGGAAGACTGTGAGATTGGTTCCATTTGGG
GGCCATTGGATCTATGGAACTACAAGCTCATTTAAATAGCCATCTTCAAGACCAATGTATTTTTGCTTTT
TAAACAAAAATTTTTACTTTGTGGCTGAATCCAAGGCAACTGTTCTGTCATTATCAATTACTCTCTGTTT
TAATCATGGGCTTGGTATGTTAGTAACTGTTTTTATATTCACTTCCATCAGGGGATGCCAGCTCTTCACT
GTGACTCATTTTGATGTACAGTTTCTTTCTGAACTAAAACCATTTGTGAATATATCAAGCTCTTTTTTTG
TATCTGATTTTGATCCAAATAAAACCTCGAATGGCTTCCTGACTGTTAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30443236 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30443236 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007082 same experiment
 EMAGE:31758 same embryo
 EMAGE:29947 same embryo
 EMAGE:31671 same embryo
 EMAGE:31285 same embryo
 EMAGE:31678 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS