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EMAGE:31829

Crtac1 ( MGI:1920082)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829
euxassay_001956_01 euxassay_001956_02 euxassay_001956_03 euxassay_001956_04 euxassay_001956_05
EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829
euxassay_001956_06 euxassay_001956_07 euxassay_001956_08 euxassay_001956_09 euxassay_001956_10
EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829
euxassay_001956_11 euxassay_001956_12 euxassay_001956_13 euxassay_001956_14 euxassay_001956_15
EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829 EMAGE:31829
euxassay_001956_16 euxassay_001956_17 euxassay_001956_18 euxassay_001956_19 euxassay_001956_20
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euxassay_001956_21 euxassay_001956_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12
olfactory cortex marginal layer
strong strong
regionalstrong expression: see section 10 11 13 14 15 16 17
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 14 15 moderate expression: see section 05 06
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 15 moderate expression: see section 06
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14
dorsal root ganglion
strong strong
regionalstrong expression: see section 06 07 11 12 13 14 moderate expression: see section 03 04 05
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 17 18
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 16
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 18 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 07 14
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 07 16
trigeminal v ganglion
strong strong
regionalstrong expression: see section 02 03 04 16 17 18 19 moderate expression: see section 05 06 07
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 17 18
telencephalon mantle layer
strong strong
regionalstrong expression: see section 02 03 19 20
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4549
Entity Detected:Crtac1, ( MGI:1920082)
Sequence:sense strand is shown

>T4549
CTTGTCTGTGTATGTGGCCACACCTGAGACAAGTTGTTCAAATTCCGAAATAACCGGTGGGAAGACATCC
TGAGTGATGACGTCAATGTGGCCCGTGGAGTGGCCAGCCTCTTTGCGGGACGCTCCGTGGCCTGTGTAGA
CAGGACGGGCTCTGGAAGATACTCTATCTACATAGCCAACTATGCCTATGGTGATGTGGGGCCTGATGCC
CTCATCGAAATGGACCCTGAGGCCAGTGACCTGTCCCGGGGGATCCTGGCACTCAGGGACGTGGCGGCTG
AGGCTGGGGTCAGCAAGTACACAGCGGGCCGGGGTGTCAGCGTAGGCCCCATTCTCAGCAGCAGTGCCTC
AGATATCTTCTGTGACAACGAGAACGGGCCCAACTTCCTCTTCCACAACCAAGGCAACGGTACCTTTGTG
GATACTGCGGCCAGTGCCGGCGTAGACGACCCTCATCAGCATGGCCGAGGTGTGGCCCTGGCAGATTTCA
ACCGTGACGGCAAAGTAGACATCGTCTATGGCAACTGGAACGGCCCACATCGCCTCTATCTACAGATGAG
TGCTCACGGGAAGGTCCGGTTTCGGGACATTGCTTCGCCCAAGTTCTCCACGCCCTCCCCTGTCCGGACT
GTCATTGCTGCCGACTTTGACAATGACCAGGAACTGGAAGTCTTCTTCAACAACATTGCCTACAGAAGCT
CCTCAGCCAATCGCCTCTTCCGTGTCATCCGCAGGGAGCACGGGGACCCTCTCATCGAGGAGCTCAATCC
TGGTGACGCCCTAGAGCCTGAGGGCCGGGGTACAGGGGGCGTAGTGACCGACTTCGATGGCGATGGGATG
CTGGATCTCATCTTGTCACATGGAGAGTCCATGGCTCAGCCACTGTCTGTCTTCCGGGGAAATCAGGGCT
TCAGCAACAACTGGTTGCGTGTGGTACCTCGCACCCGGTTCGGGGCCTTTGCCAGGGGCGCCAAGGTTGT
ACTCTACACCAAGAAGAGTGGGGCGCACCTACGGATCATTGATGGGGGTTCCGGCTACCTGTGTGAAATG
GAGCCTGTGGCACATTTTGGCCTGGGACGGGATGAAGCCAGCAGTGTGGAGGTGACGTGGCCAGATGGCA
AGATGGTGAGCCGAAGTGTGGCCAGCGAGGAGATGAACTCGGTGTTGGAGATCCTCTACCCCCAGGATGA
GGACAAACTTCAGAACACAGCCCCACTAGAGTGCGGCCAAGGATTCTCCCAGCAGGACAATGGCCATTGC
ATGGACACCAATGAATGCATCCAGTTCCCATTTGTGTGCCCTCGAGACAAACCCGTATGTGTCAACACCT
ATGGAAGCTACAGGTGCCGGACCAATAAAAGATGCAATCGGGGCTATGAACCCAATGAAGATGGCACAGC
CTGTGTGGCTCAAGTGGCCTTTTTAGGTGGGTACTCATCGGCTGCCTTTAGACTCTCTGAGCCTCTCTCT
CAGGCCTCGTATCTTTCTCTAGGCCTGGGACTTTGCCTTCAGTTATATGCACTTTAAATCCCATCAAAAA
AAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4976509 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4976509 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001956 same experiment
 EMAGE:29480 same embryo
 EMAGE:31350 same embryo
 EMAGE:31348 same embryo
 EMAGE:31357 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS