EMAGE mouse gene expression spatial database

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EMAGE:31998

Agc1 ( MGI:99602)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000376_21	euxassay_000376_01	euxassay_000376_02	euxassay_000376_03	euxassay_000376_04

euxassay_000376_05	euxassay_000376_06	euxassay_000376_07	euxassay_000376_08	euxassay_000376_09

euxassay_000376_10	euxassay_000376_11	euxassay_000376_12	euxassay_000376_13	euxassay_000376_14

euxassay_000376_15	euxassay_000376_16	euxassay_000376_17	euxassay_000376_18	euxassay_000376_19

euxassay_000376_20	euxassay_000376_22	euxassay_000376_23	euxassay_000376_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall skeleton

weak

regional

weak expression: see section 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

cranium

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24

nasal capsule

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

axial skeleton

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3842
Entity Detected:	Agc1, ( MGI:99602)
Sequence:	sense strand is shown >T3842 TGGCCTCGAGNCAGATTCGGACGAGGGNACTATCGAAGGGGACTTCCGCTGGTCTGACGGACACTCTCTG CAATTTGAGAAGTGGCGTCCAAACCAGCCTGACAACTTCTTTGCCACCGGAGAGGACTGTGTGGTGATGA TCTGGCATGAGAGAGGCGAATGGAACGACGTCCCCTGCAATTACCAGCTGCCCTTCACGTGTAAAAAGGG CACCGTGGCCTGTGGAGACCCCCCAGTGGTGGAGCATGCTAGAACCCTCGGGCAGAAGAAAGATCGCTAC GAGATCAGCTCCCTGGTGCGGTACCAGTGCACTGAGGGCTTTGTCCAGCGCCACGTGCCCACCATCCGGT GCCAGCCCAGCGGGCACTGGGAAGAGCCTCGAATCACCTGCACAGACCCCAACACCTACAAGCACAGGCT ACAGAAGCGGAGCATGAGACCCACACGGAGGAGCCGCCCCAGCATGGCCCACTGAGAGGAGCTTCCATAA TGTGCCCAGGATGCTGAGCCCAGCGGCCAGCCAGGCTGACCGTGCATCCCACCCACATG
Notes:	The probe template was PCR amplified from IMAGE:421307 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:421307 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000376 same experiment
	EMAGE:31958 same embryo
	EMAGE:29703 same embryo
	EMAGE:29780 same embryo
	EMAGE:29708 same embryo
	EMAGE:29840 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source