EMAGE mouse gene expression spatial database

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EMAGE:32002

Abr active BCR-related gene ( MGI:107771)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006912_01	euxassay_006912_02	euxassay_006912_03	euxassay_006912_04	euxassay_006912_05

euxassay_006912_06	euxassay_006912_07	euxassay_006912_08	euxassay_006912_09	euxassay_006912_10

euxassay_006912_11	euxassay_006912_12	euxassay_006912_13	euxassay_006912_14	euxassay_006912_15

euxassay_006912_16	euxassay_006912_17	euxassay_006912_18	euxassay_006912_19	euxassay_006912_20

euxassay_006912_21	euxassay_006912_22	euxassay_006912_23	euxassay_006912_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9133
Entity Detected:	Abr, active BCR-related gene ( MGI:107771)
Sequence:	sense strand is shown >T9133 CGGGAAGGAAGGAAGCGGCTGCTTGATTCTGGAGGTTGTGCAGCAGCAGCAGCAGCAGCCAGCAAGGTGG CCGAGTGGCCGGCCTGCTGAGGAGAGCCAGGAGCCTGGAGCAATGGCTGCAGGTCCCTGCTGCAAGGATA GAGGCTGCCATGGAAATCCTGCTCATCATTCGCTTCTGCTGTAACTGCACCTATGCTCTGCTGTACAAGC CAATTGACCGAGTCACCCGGAGCACCCTGGTCCTTCACGATCTGTTGAAGCACACACCCGTGGACCACCC GGACTACCCGCTGCTTCAGGATGCTCTCCGCATCTCCCAGAATTTCCTGTCTAGCATTAACGAGGACATT GATCCTCGCCGGACTGCAGTCACAACCCCCAAGGGAGAGACTCGGCAGCTGGTGAAGGACGGCTTCCTGG TGGAAATGTCTGAGAGCTCCCGGAAGCTGCGGCATGTCTTTCTCTTCACAGATGTTCTGCTGTGTGCCAA GCTAAAGAAGACCTCAGCAGGAAAGCATCAGCAGTATGACTGTAAGTGGTACATCCCTCTGGCTGACTTA GTGTTCCCATCCCCTGAGGAGTCAGAGGCCAGTCCCCAGGTGCACCCCTTCCCAGACCACGAGCTCGAGG ACATGAAAACGAAGATCTCTGCCCTCAAGAGTGAGATCCAGAAGGAGAAAGCCAACAAAGGACAGAGCCG AGCCATTGAGCGCCTCAAAAAGAAGATGTTTGAGAATGAGTTCTTACTGCTGCTCAATTCCCCAACGATC CCTTTCCGGATACACAATCGGAATGGAAAGAGTTACCTGTTTCTTCTGTCTTCTGACTATGAGAGGTCGG AGTGGAGAGAAGCGATTCAGAAGCTACAGAAGAAGGATCTCCAGGCCTTTGTCCTGAGCTCTGTGGAGCT CCAGGTGCTCACGGGATCCTGTTTCAAGCTTAGGACTGTGCACAACATTCCTGTCACCAGCAATAAAGAT GATGATGAGTCTCCAGGACTGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGT CAGCCAATCTTTACTGTACTCTGGAGGTGGATTCCTTTGGCTATTTTGTCAGCAAAGCCAAGACCAGGGT GTTTCGGGACACGACAGAGCCCAAGTGGGATGAGGAGTTTGAGATTGAGCTGGAGGGTTCACAGTCCCTG AGGATCCTGTGTTATGAGAAGTGCTACGACAAGACCAAGGTCAACAAAGACAACAATGAGATTGTGGACA AGATCATGGGCAAGGGGCAGATCCAGCTGGATCCACAGACAGTAGAAAGCAAGAACTGGCATACGGACGT GATTGAAATGAATGGGATCAAAGTGGAATTCTCCATGAAATTTACCAGCCGTGACATGAGCCTGAAGAGG ACCCCATCCAAAAAGCAGACAGGCGTCTTTGGAGTGAAGATCAGCGTGGTGACCAAGCGGGAGCGCTCCA AGGTGCCCTACATCGTCCGGCAATGCATAGAAGAGGTGGAGAAGAGGGGCATTGAGGAGGTTGGCATCTA CAGGATATCAGGGGTGGCCACGGACATCCAGGCCCTGAAGGCTGTCTTTGATGCCAATAACAAGGACATC CTACTGATGCTGAGCGACATGGACATCAACGCCATCGCTGGGACCCTCAAGCTATACTTTCGGGAGCTGC CTGAGCCCCTCCTTACAGATAGACTTTATCCAGCCTTCATGGAGGGCATTGCCCTGTCAGACCCTGCTGC CAAGGAGAACTGCATGATGCACCTACTCCGCTCCCTGCCTGACCCCAACCTCATCACCTTCCTTTTCCTG CTGGAACACTTGAAAAGGGTTGCTGAGAAGGAGCCCATCAACAAGATGTCACTTCACAACCTTGCCACGG TGTTTGGCCCCACGTTACTGAGACCATCAGAAGTGGAGAGCAAAGCACATCTCACATCAGCTGCAGACAT CTGGTCCCATGATGTCATGGCCCAGGTCCAGGTCCTCCTCTACTACCTGCAGCATCCCCCCATTTCCTTC GCAGAACTGAAGCGGAACACACTGTACTTCTCCACAGACGTGTAGCCTGGGGTGGGCAGCTGTGGGGGCG GCCAGGGGCCAGCCCTCCAGCCGGGCCCCGACATAGACTTGAAAGACTGCAACAGAAACTCCCCAGCCTA GCACTCCAGAGTCCAAGGGAAGCCACCTTCCAAGAACTGGAACATGTGCATCTTTTGTGCCACCTTGTAC AAAGCCAGCTGACCAGCCCCCGCCTCCTCACCACACCCCAGCTCCTATCTTCTGTACCCGAGTGTGTCTG ATGCTCCGTGCTGTCCAGGAATTGTTTTATGTATTATTGTCATTGCAGAAAAGGTAGTGGCCGAGCTGGT ATGGGCACAGACAGATTGCTTTGTTCTTTCATTTCTCCCAGCACTCACTTTCTGCCTGAGTCCGGTCCAA GGACTTTTACTTTGCGCTTCGTAACTACTGCCAGCTTCTCCTCTCCTGGCCCTGCTCCCAGATTGCAGCC TCCCTGCAGTGTCTGCCCTTGCCACCTCCCAGCCTGCTGCATGCATGTGCAGCATGGGCTTTTGCTCCAT CACCGCAAAGTTCTGCAGGGGCCCTGGGCAGTGCTGGCCTGTCTGACGTGCTGCCCTTGGCCTCCTTGCT CTCCACGTGTGGAAGCGCATCTGCTTTCCTTGCTGTCTGTGCAAATGCTGAACAAGGAGACAGACACACA CTAATCCTCAGCACAGAGCTGGAAAGCTCATTTCTGGAATGTTCTATTTGAGAATTGCCATTTCTGGGGT TTGTCTGTTCTATCTCCTGCCTACCAGTCGGTATCTTCAGCTGTTTCTTCTATTGCTGGATTTTCTGTTC CTTTTCCCCAGTGTTCTGTTTTCTTTGAGTGTAATGACCCACAAGTGTCCTGCCATTAAGAGAGGTCAGG ATTGCACCTGAAGGGTATGGTGGGCACCCAGGCTGTGAAGGAACAAGATGAGTGGAGCGGCCAGCAGTGG CAAGTCAAGGGAAGCCAGTCTTCAGTGTAAGGACCCGCAGTGGCTATCCTAGTGTACATTCTCCTGGAGA GCAGCTTCTGTGGCTGAATCAGGTGTCCAGGTGAAGACAGGCCCTGTCCTGGAACCTGGAATCACACATA CCTCGTGGTACTTTTGCCTTAGATTTTACACTCTCAACAGCCAAGCACTGCCACGGGGCAGCCACAATGC CATGCGTGCTCCAGAGCCTAGGAACAGCAGACAGAACCCAGTTGGAATGCTTCTACCCTGGCTTCTAAAT TCAGGCCTGTGGGTAATCCCAAGTATACAGGAAATTGTGGGGTAGGCTGTCTCTCGTGAATTGAGTTCTG CTGGGGCTATAGAGCATCGGGTCGGCAGCTGTCATTCCCAGCTCTGTGACCTACTGCTTGGTACTAAAAA GGAGTTTTCTTCCAAAAGCACTGTTTCCAGTCTGAGTAGGAGCTACCAGCCTGTGGCAATCAGCAGGGAA AGACAGGTGCTCCTGTCATAGGGGCTTGAGCTACCACAGCTACCACACAGCTTTCTGGATCTTCTATATG GCAGTTCATTCTTGGCCCTCAGGATTGGGGAGACCAGCTCTCATTGTGTGTGCTCAGGGCTAAGTCTAAA CAAGGGTTTGCTGGCCCCCAAGCTGTTGTTTGTTAGGTGAGGTTAAGTAGGGCTCTTCTGGTCCTGTGCA TACCTATTGCCTTTGACAGAACGGTACAGTTTGTTTATGCAGGAGGATGGCATGCCAACTTCCCAGCTGC CTACTAGCAGTTTAACAAGGAGCCTTGCTGTCTCAGACAGGTGAAAGAAAATGTCATGCATGTAAAGATC ACATCTGCCACTACCTTGGCACTCAGGTGCTGGTATGTGCATTCTTGCTTCCAAAGGATTGACAGTAACA AATTCTCAAAGCTTGACTGGAGCTGGCTGCAGCTCAAAACAGTGCCTCCTGCCTCTTTAGAGCGGGGCTG CTCGGCAGCAGTGACTGCAGCCCCAGCTTGCTTAAGCAGTGTAACAGCAGGGAAGCGGGAGGAGCTGAGA ACTATTAGAAGTTCCTATTATTTATAAATTCCACCCAAAGTGGACAGAGTCAGGTGCCAGACCTGATCTG AAGTGCACCAAGGAAGTATCTGGTGGTTCTCCTGAGATGGGGGCCTTGCAGCCCAGCACCCCCTCACTGC CAGCAGGGCACCAGGAGACAGGGTCCAGGAATCCAGGTATGCCTGTACTTGCTTGAGCAACGACTGAGGG CTGCCAGCTTTTGCTGACAAGGTAGGATGGGACCCCCAATAAGTGGGTATGGATATATTTTTTTTTGTCT CTGTTCTGTTTAAACTTATGTAGATTATTATAAATTGATGTAAACCATGTGAGAGGGAAATGTTAATAAA AAAATTGCAAAGCCTCAACATTTGCACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825281 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825281 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006912 same experiment
	EMAGE:29560 same embryo
	EMAGE:31175 same embryo
	EMAGE:29561 same embryo
	EMAGE:31158 same embryo
	EMAGE:32004 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source