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EMAGE:32114

Fabp5 ( MGI:101790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32114 EMAGE:32114 EMAGE:32114 EMAGE:32114 EMAGE:32114
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euxassay_002194_11 euxassay_002194_12 euxassay_002194_13 euxassay_002194_14 euxassay_002194_15
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 06 11
axial skeleton
strong strong
regionalstrong expression: see section 07 08 09 10
axial skeleton tail region
strong strong
regionalstrong expression: see section 09 10 11 12
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 04 14
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09
dorsal root ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 10 11 12
lower jaw molar
strong strong
regionalstrong expression: see section 06 17
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 05 06 13
upper jaw molar
strong strong
regionalstrong expression: see section 05 06 17
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 07 08 09 10 11
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 14 15 16 17 18 19
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 08 19 20
naris
strong strong
regionalstrong expression: see section 12 13 15 16 17
liver lobe
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
lower jaw incisor
strong strong
regionalstrong expression: see section 09 10 11 13 14 15
upper jaw incisor
strong strong
regionalstrong expression: see section 11 12 14 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6064
Entity Detected:Fabp5, ( MGI:101790)
Sequence:sense strand is shown

>T6064
CGCGTCTCTGCTGCTTTTGTGCTCTCCCTCCCGCCATGGCCAGCCTTAAGGATCTCGAAGGGAAGTGGCG
CCTGATGGAAAGCCACGGCTTTGAGGAGTACATGAAAGAGCTAGGAGTAGGACTGGCTCTTAGGAAGATG
GCTGCCATGGCCAAGCCAGACTGTATCATTACGTGTGATGGCAACAACATCACGGTCAAAACCGAGAGCA
CAGTGAAGACGACCGTGTTCTCTTGTAACCTGGGAGAGAAGTTTGATGAAACGACAGCTGATGGCAGAAA
AACTGAGACGGTCTGCACCTTCCAAGACGGTGCCCTGGTCCAGCACCAGCAATGGGACGGGAAGGAGAGC
ACGATAACAAGAAAACTGAAGGATGGGAAGATGATCGTGGAGTGTGTCATGAACAATGCCACCTGCACTC
GGGTCTATGAGAAGGTGCAATGAGGGCTTCCTCGTCATCCTGGACAGGAGTTAGCTGCCAGAGTGAATAT
GCTCAATTCAGTGAGCGGTCAAATGCAACAAACAGCTTCACTTCTTTGGTTTTATTTTTCATGACTGTTG
AGTTCTCTTTATCACAAACACTTTACATGGACCTTCATGTCAAACTTGGTTTACCCAGGATCATTCCTTT
GGTTAGTAAATAAACGTGTTAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3490535 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490535 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002194 same experiment
 EMAGE:32093 same embryo
 EMAGE:31196 same embryo
 EMAGE:30212 same embryo
 EMAGE:29862 same embryo
 EMAGE:31666 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS