EMAGE mouse gene expression spatial database

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EMAGE:32138

Ascc1 ( MGI:1916340)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001078_01	euxassay_001078_02	euxassay_001078_03	euxassay_001078_04	euxassay_001078_05

euxassay_001078_06	euxassay_001078_07	euxassay_001078_08	euxassay_001078_09	euxassay_001078_10

euxassay_001078_11	euxassay_001078_12	euxassay_001078_13	euxassay_001078_14	euxassay_001078_15

euxassay_001078_16	euxassay_001078_17	euxassay_001078_18	euxassay_001078_19	euxassay_001078_20

euxassay_001078_21	euxassay_001078_22	euxassay_001078_23	euxassay_001078_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

weak

regional

weak expression: see section 12 13 14 15 16

thymus primordium

weak

homogeneous

weak expression: see section 12 13 14 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 20

hindgut

weak

regional

weak expression: see section 13 14 15

nasal capsule

weak

regional

weak expression: see section 10 11 12 13 16 17 18 19 20

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17

bladder

weak

regional

weak expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4261
Entity Detected:	Ascc1, ( MGI:1916340)
Sequence:	sense strand is shown >T4261 GCCTTTGCATACGGCCGGCGCGGGCGAGCCTCCAGGGTCTCTTCCTACGCTCCCAGTTAGTCCTGGCAGG ACTTTCCTGGAACGTCATTTGTCATGGATGTTCTACGCCCACAGATTGTAACGTTCGATGGACGGAATTA TAGAAAGAATCCCATCCAGGAGAAACAGTACCAACATGAAGAAGACGAGGATTTCTATCCTGACTCCATG GAATACTCTGATGAGCCCTGCGGTGCCTACGAGGTGGCACAGACCCCTCACGGATTCCGGGCCACCGTCA GTGCCCCTAGCCTGCTCTACAAGCACATAGTTGGAAAGAGAGGGGATACCAAGAAAAAGATAGAAGTGGA GACTAAAACATCTATTAACATTCCGAAGCATGGGCATGAAGGGGAAATCGTGATCACTGGTCAGCATCGA AATGGCGTGGTCTCAGCGCGAACACGGATCGACGTGCTCCTGGACACGTTCAGGAGGAGGCAGCCTTTTA CCCACTTCCTTTCCTTCTTCCTCAATGAAGTTGAGGTTCAGGAACGGTTTCTGATGTTCCAGGAGGAGGT GCTGAGGAAGTGCTCCAAGGATCGTGGAGTTGATAGCACCATTTTTCAGAACCCCAAAAAGCTTCACCTA ACTATTGGGATGTTGGTACTTCTAAGTGAGCAGGAGATCCAGCAGACGTGTGAGATCCTGCAGCGCTGTA AAGAGGAATTCATTAATGACATTTCTGGAGGCAGACCCCTGGAAGTCGAGATGGCAGGCATCGAATACAT GAATGACGATCCTGCTATGGTGGATGTTCTTTATGCCAAAGTCCACATGAAAGATGGCTCCAACAGGCTG CAGGAGCTGGTTGATCGAGTGCTGGAGCGTTTTCAGTCCCTGGGACTCATCGTGAAAGAGTGGACCAGTG TGAAACTGCACGCCACGGTCATGAACACACTGCTCAGGAAAGACCCCAACGCGGAAGGCAGGTACAATCT CTACACAGCAGATGGCAAATATATCTTCAAGGAGAGAGAGTCTTTTGATGGCCGGAACATTTTAAAGACA TTTGAGAACTTCTACTTTGGTTCCCTGAGGCTCAATTCTATTCACATCTCCCAGAGGTTCACGGTGGACA GCTTTGGAAACTATGCCTCCTGCGGACATGTTGACTTCTCCTGACGCGGGAGAGCAGCAGAGAACCATGA CTGACTGGATGTCGGGAGCCTGGCAGTGTGGCAGCAGCAGGCCCTTTGGCTCCAGTTGACCGGCAGAGAA GGGTGCTCCAGCCCATGCTCCTCCCTCCTGGCTCTCATTCTCCCCCTCCTCCTTGGCTTCTGTCCTTCAT TCCTTACCCTCCACCCTCCTTTCTGTGACTCTGCTCCTGAGCTCTGTGGTGTGGCCGACCACTGTGCTGT GCGCTGTGAGATGGCACAGACCGTCTGCAAATAAATGAGCTTCCCAGGTGAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503445 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503445 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001078 same experiment
	EMAGE:32154 same embryo
	EMAGE:31153 same embryo
	EMAGE:30949 same embryo
	EMAGE:32139 same embryo
	EMAGE:31199 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source