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EMAGE:32220

Slitrk6 SLIT and NTRK-like family, member 6 ( MGI:2443198)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32220 EMAGE:32220 EMAGE:32220 EMAGE:32220 EMAGE:32220
euxassay_012161_01 euxassay_012161_02 euxassay_012161_03 euxassay_012161_04 euxassay_012161_05
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euxassay_012161_06 euxassay_012161_07 euxassay_012161_08 euxassay_012161_09 euxassay_012161_10
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euxassay_012161_11 euxassay_012161_12 euxassay_012161_13 euxassay_012161_14 euxassay_012161_15
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euxassay_012161_16 euxassay_012161_17 euxassay_012161_18 euxassay_012161_19 euxassay_012161_20
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euxassay_012161_21 euxassay_012161_22 euxassay_012161_23 euxassay_012161_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
endolymphatic duct
strong strong
regionalstrong expression: see section 05
utricle
strong strong
regionalstrong expression: see section 03 04 05 06 17 18 19 20
right lung
weak weak
regionalweak expression: see section 12 13 14 15 16 17 18 19 20 21
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09
rectum
moderate moderate
regionalmoderate expression: see section 13
left lung
moderate moderate
regionalmoderate expression: see section 03 04 weak expression: see section 05 06 07 08 09 10 12
cochlea
strong strong
regionalstrong expression: see section 06 07 16 17
midgut
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
thalamus mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 14 15 16 17
perioptic mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 19 20 21 22 23 24
tongue mesenchyme
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14
esophagus
moderate moderate
regionalmoderate expression: see section 10 11 12
vibrissa
moderate moderate
regionalmoderate expression: see section 03 04 05 06 17 18 19 20 21 22 23
dermis
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
nasal cavity
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16
pharynx
moderate moderate
regionalmoderate expression: see section 11 12
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37057
Entity Detected:Slitrk6, SLIT and NTRK-like family, member 6 ( MGI:2443198)
Sequence:sense strand is shown

>T37057
TCCTCCACCGTAGGAGAAGATATAAGAAGAAAAAAGTAGAGGAACAACTGAGAGACAACAGTCCTGTACA
CTTGCAGTACAGCATGTATGGTCATAAAACAACCCACCATACAACGGAAAGACCCTCTTCCTCTCTCTAT
GAACAACACATGGTGAGCCCAATGGTTCATGTCTACAGAAGTCCATCCTTTGGTCCAAAGCATTTGGAAG
AAGTTGAGGAAAGAAATGATAAAGAAGGAAATGATGCAAAACATCTTCAAAGGAGCCTGTTAGAACGAGA
AAATCATTCACCACTCACAGGTTCAAATATGAAATACAAAACTACAGACCAATCAACAGATTTCATATCC
TTTCAGGATGCAAGCTTATTATATAGGAACATTTTGGAGAAAGAGAGGGAACTTCAGCAACTTGGCATCA
CAGAGTACCTAAGAAAAAATCTTGCTCAGCTCCAGCCAGAGGTGGAGGTTAACTATCCTGGAGCCCACGA
GGAGCTAAAATTAATGGAAACGTTGATGTACTCACGACCAAGGAAGGTGTTGGTAGAACAGACTAAAAAT
GAATACTTTGAGCTCAAAGCTAACTTGCATGCTGAACCCGACTACTTAGAAGTCTTGGAGCAGCAAACAT
AGAGGGGCTGCTTGTGGGCTTTTGCAGAAATGCTGGGATTCTGACTTAAGTTCACACTTTTGAAATAAGT
GCCTTACGTGGGTGTTATCAATCAGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93654. Forward Primer - name:093654_F_cDNA_Slitrk6, sequence:TCCTCCACCGTAGGAGAAGATA; Reverse Primer - name:093654_N_SP6_cDNA_Slitrk6, sequence:TTCTGATTGATAACACCCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012161 same experiment
 EMAGE:31913 same embryo
 EMAGE:32221 same embryo
 EMAGE:31917 same embryo
 EMAGE:30784 same embryo
 EMAGE:32223 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS