EMAGE mouse gene expression spatial database

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EMAGE:7006

A030009H04Rik RIKEN cDNA A030009H04 gene ( MGI:1915359)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002554. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002554_01	euxassay_002554_02	euxassay_002554_03	euxassay_002554_04

euxassay_002554_05	euxassay_002554_06	euxassay_002554_07	euxassay_002554_08	euxassay_002554_09

euxassay_002554_10	euxassay_002554_11	euxassay_002554_12	euxassay_002554_13	euxassay_002554_14

euxassay_002554_15	euxassay_002554_16	euxassay_002554_17	euxassay_002554_18	euxassay_002554_19

euxassay_002554_20	euxassay_002554_21	euxassay_002554_22	euxassay_002554_23	euxassay_002554_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7006_wholemount_strong.wlz

7006_wholemount_moderate.wlz

7006_wholemount_weak.wlz

7006_wholemount_possible.wlz

7006_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7006_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon

moderate

regional

moderate expression: see section 01

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 02

telencephalon marginal layer

strong

regional

strong expression: see section 04 05 22 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 15 16 17 18

hindbrain

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord

strong

homogeneous

strong expression: see section 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5187
Entity Detected:	A030009H04Rik, RIKEN cDNA A030009H04 gene ( MGI:1915359)
Sequence:	sense strand is shown >T5187 CCACGCGTCCGCTAGACCCTGTCTTGTGGTCACGGCTGGAGGAAAAAGCCCGGGCTGAGCGTGAAGGAGA CCCGATGGGGAGCCCGGCCAAAGACAGCGGAGAGGATGGAGAGGACGACGACGGGGAAGCGGAGAGCGAG AAGGGGGCGGGACCTCCAAGTGCGGGTTGGCGCGCGCTTCGCCGAATTTTGGACAGGGTCCTGGCGCCCG CACGCCGCTTGCGGCGTCCGTTGCCTAGCAATGTGCTCTACTGTCCAGAGGTCAAGGACATTGCCCACAT GACCCGTTGTACGCTGTAACCAAGGAACCCCAGAGCTATGGCAGAAAGCAGGTGGGAGCAGCATCCTCAG GCGTGCTCTGACACAAAAGGTTGATGTCAAGACTACCTGGAAATCAAGGCTGATGCTGGAATTGGTACGT GCGTGATGGAGATGGAGCTCAACAGAAAGGACCCTAGATGGTGTGGATTCTGAGCGGCGAAGGCTCCTAG ACTGTGCCAGCATTATATTTTCAGGATGGAGTGGCCAAGGGATAATAGAAACAAAGCCTACCATAATGTA GGAAGAAAAAAAAAATAACAAAGCTTTGGCGTTTGTTCAGAGTCTGTGGTGTAAGCAAAGGATCCAATTT TAGCAGTGGTCTGTGGTTGACAATGTGTACTGTGGAAAAAAGGACAGGACAGGGGATAAGCTAATTTATA TGTCATGTCAATCTCACTTATCTGGTTGTGTTTGCTGGCATGCCATCTTCCCACTCTTGTTACCGTGCTC CAGCAATTACTGAGTTGACTCCCTGCTTATGATTGGGAATCTTGCATATACAACCTCCCTTCAGCTGGAT AGGGCAGGTATGAAAACATCCAAGGGGCATTTATTTACATGAGGCCTTATTAAAGGCTTATCAAAGATGG GTGAGCCATTTGATAAAGGAAGCAAAATTTAGGCCTTTGTTATCTTTACTGACTCTGTAGCAATACTGGG GAAAAGAAAAAAAAAGATGCTCACATAATTTTAATTTGTGTCCCATAATGGGACATTGTTTACTGTATGT AGTTAGTTCTATTCTAAAGTCCTGTAAATATCTTACTGTAGACTTAAAATCAAGACCAGCAGTGTTGGTT GGTCTCACAGCTTGCTAGAAATGCAGAATTTCAGGACCCCAGTCCACATTCCCCGGTAGTTTTAGAATCC CAGGTATGTGTATGTAAGTTAAGAATCACTGGTTTAGCTCTTAGTCACTCTGAGATGGCATCATCAGCTG TAGGTGGAACATTTAAAACACTAGTGACGCATTAAAGTGCCAAAGTGACATTCTTTTGAGAAGAAAAATT TTTCTGAGCTACAAATGTGAATTTACAAGGCCTCAGTTTTCAGAGGTCTTCAAGATTTTGTTTTTAATGA CAAATAATGTATGTAGTTCTAGGGTATAAACCTCCATTTGTAAAATGTTTTGTCTTTTTCCTGTCCTTCG GTTCCAGCTGAAATCTCCCCTTTTGGAGGCCAGCATGCATCTGTGGGAATGTATATGTTTTCTCTATACG TGTTGCCAAAGGGAATGCTATGTTTATTTTCTGCTATTTTATGTTTTGAGTATTTCTAGAGTATAAATTT CAAGTGACTATCTCCAGAGCCGATAGCAGTGTTAATGTTATATGTATTCAAAAAAATGTTTGCTGAATTT ATTTTGAATGTAATAGTCTCTAGAATAAAGTATCTGGGCTCATTCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5352453 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5352453 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7010 same embryo
	EMAGE:7007 same embryo
	EMAGE:7009 same embryo
	EMAGE:7008 same embryo
	EMAGE:7011 same embryo
	EurExpress:euxassay_002554 same experiment
	MGI:4822814 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source