EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7077

Fanci Fanconi anemia, complementation group I ( MGI:2384790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002481_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7077_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7077_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5082
Entity Detected:	Fanci, Fanconi anemia, complementation group I ( MGI:2384790)
Sequence:	sense strand is shown >T5082 CCACGCGTCCGAGAGCGGGAATTTTGGAAGGATCCCGAGCTGAGGTTGAGTTCTCTGATATGAGCAACCA TGGATCTGAAAATTTTATCTCTAGCAACAGATAAAACTACAGATAAACTGCAGGAGTTTCTTCAAACCCT GAAAGATGATGATTTGGCCAGTCTCCTTCAGAACCAAGCGGTGAAAGGAAGAGCTGTTGGAACGCTCCTG AGAGCCGTCCTCAAAGGCTCCCCCTGCTCTGAGGAAGATGGAGCCCTTAGGAGATACAAGATATATTCTT GCTGTATCCAGTTGGTTGAATCGGGAGATTTGCAGCAAGATGTCGCATCAGAGATCATAGGATTACTAAT GCTGGAGGTCCACCATTTTCCAGGCCCATTACTTGTTGACTTGGCTAGTGACTTTGTGGGTGCTGTCCGA GAGGACCGCCTAGTGAATGGAAAATCTTTGGAGTTATTACCCATCATCCTTACTGCCTTGGCCACCAAGA AGGAAGTCCTCGCTTGTGGGAAAGGTGATTTGAATGGGGAAGAATATAAGAGGCAGTTGATTGACACCCT GTGTTCTGTCAGATGGCCTCAACGGTACATGATCCAGCTCACTTCTGTGTTCAAGGATGTCTGTCTAACG CCAGAAGAGATGAATCTCGTGGTGGCCAAAGTGTTGACGATGTTCTCAAAGTTGAATCTTCAAGAAATTC CACCTTTGGTCTATCAGCTTCTGGTTCTCTCTTCCAAGGGAAGCAGAAGGAGTGTTCTGGACGGAATCAT AGCCTTCTTCAGGGAGCTGGATAAGCAGCACAGGGAGGAACAGAGCAGCGATGAGTTGTCAGAGCTTATT ACTGCCCCAGCAGACGAACTCTATCACGTGGAAGGCACCGTTATTCTGCACATCGTATTTGCCATCAAAC TGGACTGTGAATTAGGCAGAGAACTTCTGAAACACTTGAAGGCTGGACAGCAGGGGGATCCCAGTAAATG CCTATGTCCCTTCAGCATCGCTCTTCTCCTCTCTCTCACGAGAATACAGAGATTTGAGGAACAGGTGTTT GATCTTTTAAAGACTTCTGTTGTAAAAAGCTTCAAGGATCTTCAGCTCCTCCAAGGCTCCAAGTTTCTTC AGACTCTCGTCCCCCAGAGAACTTGTGTTTCAACCATGATCTTAGAAGTGGTGAGGAACAGACTTGTTTT CAGATATCATCATGTATGCACCTTTAATTCTTCAGAACTGTTCAAAAGTCACGGAAACTTTTGACTATTT GACCTTTCTGCCCCTCCAGACCGTGCAAGGGCTGCTCAAGGCGGTGCAGCCGCTTCTCAAGATCAGCATG TCGATGAGAGACTCCTTGATCCTCGTCTTGCGGAAAGCTATGTTTGCCAGCCAGCTGGATGCCCGGAAAT CTGCAGTTGCTGGGTTTTTGCTGCTCCTAAAGAACTTTAAAGTCCTAGGCAGCCTGCCGTCCTCACAGTG CACACAGTCTATTGGTGTCACGCAGGTGCGCGTGGACGTTCACAGCCGATACAGCGCTGTCGCCAACGAG ACCTTCTGCCTTGAGATCATAGACAGCTTGAAACGAAGCTTAGGGCAGCAGGCAGACATCCGGCTCATGC TTTACGACGGTTTCTACGATGTCCTTCGACGGAACTCTCAGCTGGCTAGTTCAATCATGCAGACTCTCTT CTCACAGTTAAAACAGTTCTATGAGCCAGAGCCTGATCTGCTACCTCCTCTGAAACTAGGAGCTTGTGTT CTGACCCAAGGAAGTCAGATCTTTCTACAAGAGCCGCTGGATCATCTGCTGTCTTGCATTCAGCATTGCT TGGCCTGGTATAAGAGCAGAGTCGTCCCCCTGCAACAAGGAGATGAAGGAGAGGAGGAGGAGGAGGAGTT GTACTCCGAGCTGGATGATATGTTGGAGTCCATTACTGTCAGAATGATCAAGAGTGAGCTGGAAGACTTT GAGCTGGATAAATCAGCAGATTTCTCTCAGAACACTAATGTCGGCATCAAGAATAATATCTGTGCGTGTC TCATCATGGGAGTGTGTGAAGTTCTAATGGAATATAATTTCTCCATAAGTAACTTCAGCAAGAGTAAGTT TGAGGAGATCCTGAGCCTCTTCACATGTTACAAGAAGTTCTCTGACATCCTCAGTGAGAAAGCTGGCAAA GGCAAGGCTAAGATGACCAGTAAAGTCAGCGACAGTCTCCTGTCCTTGAAGTTTGTGTCTGATCTCCTCA CTGCTCTCTTCAGAGACAGCATCCAGAGTCATGAAGAGAGTCTGTCTGTCCTGCGGTCCAGCGGCGAGTT TATGCACTATGCAGTGAACGTCGCTTTGCAGAAGATTCAGCAGCTAATACGAACAGGGCATGTGAGTGGC CCTGATGGTCAGAACCCAGACAAGATCTTTCAGAACCTCTGTGACATAACTCGGGTCCTGCTGTGGAGAT ACACATCAATTCCTACATCCGTGGAGGAGTCGGGGAAGAAGGAGAAGGGAAAGAGCATCTCGCTGTTGTG CTTGGAGGGCTTGCAGAAGACGTTCAGTGTGGTGCTGCAGTTCTACCAGCCCAAGGTCCAGCAGTTTCTC CAGGCTCTGGATGTCATGGGCACAGAAGAAGAAGAAGCAGGTGTCACTGTCACTCAGAGGGCCTCGTTCC AGATCCGGCAGTTTCAGAGGTCGTTGTTGAATCTGCTGAGCAGCGAAGAGGACGATTTTAACAGTAAAGA GGCCCTCCTGCTCATCGCGGTGCTGTCCACCTTGTCCAGGCTGCTGGAGCCCACCTCTCCTCAGTTTGTG CAGATGTTATCCTGGACATCTAAGATTTGCAAGGAATACAGCCAGGAGGATGCCTCATTTTGCAAGAGTT TGATGAACCTGTTCTTCAGCCTGCATGTCTTATATAAGAGTCCTGTCACCCTGCTTCGTGACTTGTCCCA GGATATCCATGGGCAGCTGGGAGACATAGATCAAGATGTAGAGATTGAGAAGACAGACCACTTTGCTGTG GTGAATTTGAGAACGGCTGCCCCCACTGTCTGTTTACTTGTTCTGAGTCAGGCTGAGAAAGTCCTAGAAG AAGTGGACTGGTTAATCGCCAAGATCAAGGGCTCAGCAAACCAAGAAACGCTATCAGATAAAGTGACTCC AGAAGATGCCTCTTCTCAAGCCGTTCCACCAACTCTTCTCATTGAGAAAGCTATCGTCATGCAACTGGGA ACTCTGGTAACGTTCTTCCATGAGCTCGTGCAGACAGCCCTGCCATCAGGCAGCTGTGTAGACACTCTAC TGAAGGGCCTGTCCAAGATCTACAGCACCCTAACAGCATTCGTCAAATATTATCTCCAGGTATGTCAGAG CTCCAGAGGAATTCCAAACACTGTGGAAAAACTGGTGAAGCTGTCGGGCTCTCATCTGACCCCCGTATGT TACTCTTTCATTTCTTATGTACAAAATAAGAGCAGTGACGCCCCAAAGTGCTCAGAAAAGGAGAAGGCTG CAGTGAGCACAACCATGGCCAAAGTTCTTCGTGAAACCAAGCCAATCCCTAACCTTGTCTTTGCCATTGA ACAGTATGAAAAATTTCTTATCCAACTGTCTAAAAAGTCCAAGGTAAAAATGTTCTTTATTTCCACTCTC ATTTACTGCTGGAAGGAGTCTAGCAAGCTCTGTGGCAGGCAGCACAGCTAGAGGTGGTCAAGGTGAATGT CATCTGGTAAAAGCAAAGGGTCCTCCAAGTACACACTGAAAGGACCTTCTGCTGTCCCAGGTAAACCTGA TGCAGCACATGAAGCTGAGTACTTCTCGGGACTTCAAGATCAAAGGCAGTGTCCTCGACATGGTTCTTCG AGAGGATGAGGAAGACGAAAACGAAGAGGGCACTGCATCAGCACATACACAGCAGGACAGAGAACCAGCC AAGAAGAGGAGGAAGAAATGCCTGAGTTAATGTGACCTTTGGGGCATCTGCTTCATTTGTGCCCAACAGT ACCCCTTGTCACACAGCCTGCACTGATGTTGGTGTCCTGGTTCTGAACGCACTGAATTGTGCCTTCCGTT AGGAGGACTTTCCTGGGCAGGTCCTGCTACAGAAAAATGGCTGCCTTTTGCAAAAAAGCACATCTGAAAG CCTGAGTTTAGGAACCTGCACCGCTCCAATAAAGCTCCACAGGAGCAAATACAGAACCTCCAGACAGAGC TAGGGTCCAGGCTGGCTTCGTTTTTCCAAGGAGCCTTTGGTGAGTTCAATTATCTGGTAAATATCCAGTG CTTCACCTGTAAGACGGTGGAAATTGGTTAGGATGCCTCCAGAATTGTGACTGAACTCAGAAATGAGAAT CTGAATGTTGGGGTCAAAGACAGTGGGTGTGAGGGAGTCTTTGAGAGTGGCAGCTTGTCTTATTATAACA CAAGCTTTCTAACCAACTCTGGCATTTTCAGCACAGATACATTTTTTCCACATAAGAATGCACTTTGGCT TATACAATAAAACTTTAAACATTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5322726 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5322726 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7072 same embryo
	EMAGE:7076 same embryo
	EMAGE:7075 same embryo
	EMAGE:7074 same embryo
	EMAGE:7073 same embryo
	EurExpress:euxassay_002481 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source