EMAGE mouse gene expression spatial database

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EMAGE:7103

Rbm28 RNA binding motif protein 28 ( MGI:2655711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002611. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002611_01	euxassay_002611_02	euxassay_002611_03	euxassay_002611_04

euxassay_002611_05	euxassay_002611_06	euxassay_002611_07	euxassay_002611_08	euxassay_002611_09

euxassay_002611_10	euxassay_002611_11	euxassay_002611_12	euxassay_002611_13	euxassay_002611_14

euxassay_002611_15	euxassay_002611_16	euxassay_002611_17	euxassay_002611_18	euxassay_002611_19

euxassay_002611_20	euxassay_002611_21	euxassay_002611_22	euxassay_002611_23	euxassay_002611_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7103_wholemount_strong.wlz

7103_wholemount_moderate.wlz

7103_wholemount_weak.wlz

7103_wholemount_possible.wlz

7103_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7103_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5226
Entity Detected:	Rbm28, RNA binding motif protein 28 ( MGI:2655711)
Sequence:	sense strand is shown >T5226 GATAGCCATGATGACGAAGAAGAAAGAGAATCAACAATAGCTTCTCCTGTGAGTGTGCACAAGAGGTATG TGCACATTCTGAGAACTAGGGAAGACCGCATTTCTCCTCTTGCCTTCACTGCGAACTTGGCCCATGTAAG AAAGCTCAGCCGCTTACATATCAGCCCCGATGATGAGATCCGAACTGTGCTGTTGCAGAGCAGTCAAGAG AGCAGCTCCCGAGGAAAGCATCGAGGAAGACGACTCCTACGAGGACAGTGACCTGGAGGAAGGAGGCAGC TCCTATGATGAGGGGACAGTGGACAGTGAGAGCAGCGCTGAAGATCAAGAGGATAAAGATGTTCCAGTCT CAGAGAAAAAGAAGAGGAAGTTACCCTCGGATGTGACGGAAGGGAAAACTGTCTTCATCAGAAATCTTTC ATTTGACTCAGAAGAAGAGGCACTTGGCGAGGTCCTCCAACAGTTTGGTGATCTTAAGTATGTCCGAGTT GTCTTGCATCCAGACACAGAGCATTCTAAAGGTTGTGCATTCGCACAATTCATGACTCAGGAAGCAGCTC AGAAATGTCTTGCAGCGGCCTCTCTAGAGGCTGAGGGTGGTGGGCTTAAACTGGACGGCCGACAGCTCAA GGTCGACTTAGCTGTGACCCGGGATGAGGCAGCAAAGCTTCAGACAAAGAAGGTGAAGAAGCCAACTGGA ACCCGGAACCTCTATCTGGCCAGAGAAGGCTTGATCCGCGCTGGGACGAAGGCTGCTGAGGGTGTGAGTG CCGCAGATATGGCCAAACGCGAGCGGTTTGAGCTACTGAAACATCAGGAACTCAAGAACCAGAATATCTT TGTCTCGCAGACCAGGCTTTGCCTGCACAATCTCCCAAAGGCTGTGGACGATAAACAGCTAAGGAAGCTG CTGCTGGAGGCCACTCGAGGAGAGAAGGGGGTTCGAATTAAGGAGTGTAGAGTGATGCGGGACCTTAAAG GAGTTCATGGGAAAATGAAGGGTCAGTCCCTGGGCTATGCCTTTGCAGAGTTCCAAAAGCATGAGCATGC CCTCAGAGCCCTCCGCCACTTCAACAACAACCCAGAGATCTTTGGGTCCCAAAAGAGACCCATAGTGGAG TTTTCTTTAGAAGACCGAAGGAAACTTAAAGTGAAGGAGCTGAGGATCCAACGCAGCCTGCAAAAAATGG AATCCAAGCCTGTGACCAGTAAGCCCCAAAAGGAGCAAAAAGAAGTTGGAAAAGACAAGCAACAGAAAGC AGTTCAGAGCGCCACACAGGATCAGAGTAAGGCGCCTGGAGAGCAGAAGGGGAAAGCCGGCTCCACCTCG TGGACAGGCTTCCAGACCAAGGCTGAAGTGGAGCAGGTGGAGCTGCCTGACGGGAAGAAGAGAAGAAAGG TCCTGGCACTCCCTTCACACCGAGGCCCCAAAATTAGGCTGCGGGACAAAGGCAAAGTGAAGTCCCTGCC TTCTAGGAAGCCAAAGCCCCAGATGGGCCAGCGGAAGCAAAAGAAGCAGCAGTTAGCGTCTTCCGTGCAG GCGCCTAAAAGAAAGGCTAAGGAGAATAAGGCAGAAGCCCGCTTCAACCAGCTGGTTGAACAATATAAGC AGAAGTTGCTGGGCCCTTCTAAAGGAGCGCCCCTCATGAAGAGAAGCAAATGGTTTGACAGCTGATGGCA CCTGCAGACTGTACAAAAGCCGTGTCTTTCCTTGAGGGAAGCAGATCCCAGATGATACACACTTTCTTGG GGACTCTAGGTTGTGTACATTCATGGTCCGTTCTCCAGTGTGGTGTTGGTTAGCCACACTGAAAAACACT ATGGAGTTTTTATAACTTACCCAATCACAGTGCTGAGTCTGTGATTGCAACTTATTTCTAATTTCTTCTC AAATGGAAACTATTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5356457 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5356457 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7107 same embryo
	EMAGE:7104 same embryo
	EMAGE:7108 same embryo
	EMAGE:7105 same embryo
	EMAGE:7106 same embryo
	EurExpress:euxassay_002611 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source