EMAGE mouse gene expression spatial database

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EMAGE:7264

Dcaf12 DDB1 and CUL4 associated factor 12 ( MGI:1916220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004378. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004378_01	euxassay_004378_02	euxassay_004378_03	euxassay_004378_04

euxassay_004378_05	euxassay_004378_06	euxassay_004378_07	euxassay_004378_08	euxassay_004378_09

euxassay_004378_10	euxassay_004378_11	euxassay_004378_12	euxassay_004378_13	euxassay_004378_14

euxassay_004378_15	euxassay_004378_16	euxassay_004378_17	euxassay_004378_18	euxassay_004378_19

euxassay_004378_21	euxassay_004378_22	euxassay_004378_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7264_wholemount_strong.wlz

7264_wholemount_moderate.wlz

7264_wholemount_weak.wlz

7264_wholemount_possible.wlz

7264_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7264_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5169
Entity Detected:	Dcaf12, DDB1 and CUL4 associated factor 12 ( MGI:1916220)
Sequence:	sense strand is shown >T5169 CCACGCGTCCGCCCACGCGTCCGGCGGTAGGAAAGCTGCTTGTTCCTGCTTTTCCTTCCCCTTCCCTTCC CCTTCCCCTTTCCCAACCCGGATTTTCCCTTCTTCCATTTACGCGCCTCATTTTGATCGACTGCTGCCCG GGATCCGAGCCATTTTCCGGGCCGGGCGCTCGAAAGCGCGCGGCCCCAGGGCCCGGTTCATGACCCGTCG CCCTGCCGCCCCTTGCCTTCTCCGCCTCATGTGGCTGCGGGGCCACGGCGGCGCTGCCCACTATGGCCCG GAAAGCAGTTAGCAGGAAGCGGAAAGCGTCTGCCTCGCCAGGAGCCGGGAGCGACGCTCAGGGCCCGCAG TTTGGCTGGGATCATTCGCTGCACAAAAGGAAAAGACTCCCCCCAGTGAAGAGATCCCTAGTGTACTACC TGAAGAACCGCGAAGTCAGGCTCCAGAATGAGACCAGCTACTCTCGCCTGTTACATGGTTATGCAGCACA ACAGCTTCCCAGTCTCCTCAAGGAGAGAGAATTTCACCTCGGGACCCTTAATAAAGTGTTTGCTTCTCAG TGGTTGAATCATAGGCAAGTGGTGTGTGGCACGAAATGCAACACACTCTTTGTCGTGGATGTCCAGACAG GCCAGATCACCAAGATTCCCATTCTGAAAGACCGGGAACCTGGAGGTGTGACTCAGCAGGGCTGTGGTAT CCATGCAATTGAACTGAATCCTTCTAGAACACTCCTAGCCACTGGAGGAGACAACCCTAACAGTCTTGCC ATCTACCGCCTGCCTACCTTAGATCCTGTGTGTGTGGGTGATGATGGACACAAGGATTGGATCTTTTCTA TTGCGTGGATCAACGATACTATGGCAGTGTCTGGCTCACGGGATGGGTCTATGGGACTCTGGGAGGTGAC AGATGATGTGTTGACCAAAAGTGATGCAAGACATAACGTGTCACCAGTCCCTGTGTATGCTCACATCACT CACAAGGCCTTAAAGGATATCCCCAAGGAGGATACAAACCCGGACAACTGCAAGGTGCGGGCTTTAGCCT TCAACAACAAGAACAAGGAGTTGGGAGCGGTATCACTAGATGGCTACTTTCATCTCTGGAAGGCTGAAAA TACCTTGTCTAAGCTCCTTTCCACCAAATTGCCATATTGTCGAGAAAACGTGTGTCTGGCCTATGGTAGT GAGTGGTCAGTGTATGCAGTGGGCTCTCAAGCGCATGTCTCCTTCTTGGATCCACGGCAGCCATCGTACA ACGTCAAGTCTGTCTGCTCCAGGGAACGAGGCAGTGGGATTCGATCAGTGAGCTTCTATGAACACATTAT CACTGTGGGCACAGGGCAGGGCTCCCTGCTGTTCTATGACATCCGAGCTCAAAGATTTTTGGAAGAGAGG CTCTCAGCTTGTTATGGGTCCAAGCCCAGACTCGCAGGGGAGAATCTGAAACTAACTACTGGCAGGGGCT GGCTGAATCATGATGAAACCTGGAGGAATTACTTTTCGGACATTGACTTCTTCCCCAATGCTGTTTACAC CCACTGCTACGACTCGTCCGGAACGAAACTCTTTGTGGCAGGGGGCCCCCTCCCTTCTGGGCTCCACGGA AACTATGCTGGACTGTGGAGTTAATGACAACTAACTTTCTCCCAAAATGCAGATTTACACTAACTTCCAC CCTCAGTTTCCTTGTTTGATTTCCCTAGCTGTGGAAGGCTCTCCTGTCTATGCAAACAGAGTTTGCTTGT GGGTCAGGCAGTTACTAGGAAGAGGCTCTATGCAGAAATCCAAAAGCTGGGACGTTCTACGTCAGTTGGT GGCTTGTTGTTCTGGGTTTTTTGTTTGTTTTCACTTTGCAGTAATTAGAATTTTACTTTTCCCTTTTCTG CTTTTTAAAGCAAACACTGTTACAATCCCTTTTTATTTTTTCCTCTCCTTCCTCTGGCTTCTTTACACTG AAATGACAAAGTCATTCTTACCCTTACTTCACTGAGGTAGGGCCCCTTTATAATCGTGTGGTGGCTGTCC GACTTTCTGTGAAAGTGTGGGAGCCTTTGTGTGCGTGTGTGTGTGTGTGTCTGTCTGTGTGTCTGTGTGC GTTTGTGTGTGTGAACTTGTGTGCCTGCTGGTACTGCATCACTGATACTACTTGGAATGAGGATTATCTG TAATTAAAGTATTTATAAAAGAAACAACTTTACTCAGAGTCCAGCTTTGGGACTAGCCTGTATCTTCTTT AAGGTCCGACATTGTAGGAAGAGAAACAGACACACCAGGAAAACCCTTGAGTTAGAGAAGCCGGATGGGA GGGGAGGCTGCATCCCAGGACTCCAAACTGTCCAGGCCTACCTGTCCTTCCACCCACGCAGGAATGCCCA TGTGTGTGTTACTTCCACTCCCGACCTCCTGTCTGCTCGCCCAGACTGGGGACTGCAGGCCACATCACAG CCTAGATAAAGACAATCCTCTCCCCAGCCTCCTGCCTCTGACAGGGTCTGATCTGACCGAGAGCAGGCAG TCTCGAGCTTGTGTGCTTACTGCTCATGACTTGGAGGAGGAACTTTTCCATTCTTGGGGTTTTGCTGGTA TTTTCTAATTTGTCTGTCCAGGAAGGTGTGGTTTGTAGCGAGTGCTTTGTGTGTGGGATCTGTTCTCAGT GCAGCAGGGCTTAGTGTTGGAAGTCACTGTCCTGCTCTGTTGGCTCATTCCTAGGGTAGTGACAGGTGGC TGTGCCAGCATCGCTGCATGTGACAGTGTAGCTGTGATTGGTAATTTGCTTGTTTCTCTCAAACTATTTA GTGTGAAGAGTTTTTTGTTGTTGTTTTTGTTTTTAACTTGCAAGCCAGTTGTTGTCTTCCGAACATCATG TGGAAAGGAAGCTCTCCCCACCCTAGTACCCTGAGACACACAGTGCTGCCTGAGAAATGCAGTCATACAC CTGCACCCAGTTTCTCAGTTGCTCCCCAGTTCTTCCATGCTCTCTTTGTGTCAAATGAGTATTCTCATAA TGGCTGGGGCTGCTGGGGAGGGAAGCGGCAGGCCCACCGTAGGACACAGCATCCTCCACAGGTTTTAGAA TAATCATCCTATAATCTAGAGAAATAGGTGCTATCAACAGGAAATTAAGCAAAATGCTGGATGCTGTGAA TCTTTTGTCTTAATTTTTTTCTATTCTTAAACTACAGGCTGTAGATGTCTTAGTTCTCACAGAACTTCTG TCTTTTTAAACTGACTTGTGTGTTTAAGTAAACAAAACTCTTGTAGGATGTTTTGTGCCATTGCCAGTCC GTCTCCTGTGATGCTTTTGATTGTCTTGAGATTTTCTGTTTTTAAAAATGTAGCATTTGACTTTTTTGCC AAGGAAAAATAAATACTATCCCAGTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5346910 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5346910 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7267 same embryo
	EMAGE:7268 same embryo
	EMAGE:7263 same embryo
	EMAGE:7266 same embryo
	EMAGE:7265 same embryo
	EurExpress:euxassay_004378 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source