EMAGE mouse gene expression spatial database

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EMAGE:7299

Llgl2 lethal giant larvae homolog 2 (Drosophila) ( MGI:1918843)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004409. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004409_01	euxassay_004409_02	euxassay_004409_03	euxassay_004409_04

euxassay_004409_05	euxassay_004409_06	euxassay_004409_07	euxassay_004409_09	euxassay_004409_10

euxassay_004409_11	euxassay_004409_12	euxassay_004409_13	euxassay_004409_14	euxassay_004409_15

euxassay_004409_16	euxassay_004409_17	euxassay_004409_18	euxassay_004409_19	euxassay_004409_20

euxassay_004409_21	euxassay_004409_22	euxassay_004409_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7299_wholemount_strong.wlz

7299_wholemount_moderate.wlz

7299_wholemount_weak.wlz

7299_wholemount_possible.wlz

7299_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7299_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 06 08 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16

pharynx

moderate

regional

moderate expression: see section 11 12 13 14

esophagus

moderate

regional

moderate expression: see section 12

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07

rectum

moderate

regional

moderate expression: see section 10 not examined expression: see section 16

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

bladder

moderate

regional

moderate expression: see section 11 12 13

metanephros

moderate

spotted

moderate expression: see section 06 08 13 14 15 16 17 weak expression: see section 05 07

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trachea

moderate

regional

moderate expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6424
Entity Detected:	Llgl2, lethal giant larvae homolog 2 (Drosophila) ( MGI:1918843)
Sequence:	sense strand is shown >T6424 CAGCCCGTGGGCCGGAGCAGGGAACCCGAGTGGTGGGTGACGGCCGCTGAGGGACGCCGAGGCCTCGGGC AGGGCCCGGCCCGCGATTGCAGGTCTGTTGTGGGGGCCACAAACTAAAGCAAGATGAGGCGGTTCCTGAG AACTGGACATGACCCTGCCCGGGAGAGGCTGAAGCGGGATCTGTTCCAGTTTAACAAGACAGTGGAGCAT GGCTTCCCACACCAGCCCAGCGCCCTTGGCTATAGCCCTTCGCTGCGCATCCTGGCCATCGGAACCCGCT CTGGAGCTGTCAAGCTATATGGCGCCCCCGGGGTAGAGTTCATGGGCCTTCACAAGGAGAACAACGCAGT GCTGCAGATCCACTTCCTGCCTGGTCAGTGTCAGCTGGTCACTCTGCTGGATGACAACAGCCTGCACCTG TGGAGCCTGAAGGTCAAGGGCGGGGTGTCAGAGCTGCAGGAAGAAGAGAGCTTCACATTGCGTGGCCCCC CAGGGGCTGCCCCCAGTGCCACGCAGGTCACTGAGATTCTACCTCACTCTTCCGGAGAACTGCTCTACCT GGGCACCGAGAGCGGCAACGTGTTTGTGGTGCAGCTCCCGGGGTTCCGCACGCTGCACGACAGGACCATC TGTTCAGATGAGGTGCTGCAATGGTTGCCAGAGGAGGCTCGCCACCGGCGAGTGTTTGAGATGGTGGAGG CTCTGCAGGAGCACCCTCGAGACCCCAACCAAATCCTCATTGGCTACAGCCGAGGCCTCGTCGTCATCTG GGACCTTCAGGGCAGCCGAGCGCTCAGTCATTTCCTCAGCAGCCAGCAACTGGAGAATGCCAGCTGGCAG CGGGATGGCTGCCTGATTGTCACCTGCCACTCCGACGGCAGCCACTGCCAGTGGCCCGTCTCCAGTGACA CCCAGAACCCAGAACCTCTGCGCAGCTCTATACCTTACGGTCCCTTTCCTTGCAAAGCTATAACGAAAAT CTTCTGGCTCACCACCAGGCAAGGGTTGCCCTTCACCATCTTCCAGGGCGGTATGCCACGGGCCAGCTAT GGGGACCGCCACTGCATCTCAGTGGTCCACAACGGCCAGCAGACGGCCTTTGACTTCACCTCCCGTGTCA TTGACTTCACTGTTCTCTCAGAGGCTGACCCTGCGGCTGCCTTTGATGACCCCTACGCCCTGGTGGTGCT AGCCGAGGAGGAACTGGTGGTCATTGACCTGCAGACACCCGGCTGGCCACCAGTCCAGCTGCCCTACCTG GCTTCCTTGCACTGTTCCGCTATCACCTGCTCCCACCATGTCTCCAACATCCCCCTGAAGCTATGGGAAC GCATCATCGCCGCGGGCAGCCGCCAGAACTCACACTTCTCCACCATGGAGTGGCCCATAGATGGTGGCAC CAGCCTGGCCCCACCGCCACCCCAGAGGGACCTGCTGCTCACAGGGCACGAGGACGGCACGGTGCGGTTC TGGGACGCCTCCGGTGTGTGCTTACGTCTGCTCTACAAACTGAGCACTGTGAGGGTGTTCCTCACAGACA CGGACCCCAGCGAGAACCTCAGTGCCCAGGGTGAGGACGAGTGGCCCCCACTCCGCAAGGTGGGCTCCTT TGATCCCTACAGCGATGATCCGCGGCTGGGCATCCAGAAGATTTTCCTCTGCAAATACAGCGGCTACCTG GCTGTGGCAGGCACGGCAGGGCAGGTGCTGGTGCTGGAGTTGAACGATGAGGCGGCCGAGCATGCTGTGG AGCAGGTGGAGGCTGACCTGCTGCAGGACCAGGAGGGTTACCGCTGGAAGGGGCACGAGCGCCTCGCCGC CCGCCCGGGGCCCGTGTGCTTTGAGGCGGGATTTCAGCCCTTTGTACTGGTGCAGTGCCAGCCCCCAGCT GTGGTCACCTCCTTGGCTCTGCACTCCGAGTGGCGGCTTGTGGCCTTTGGCACCAGCCATGGTTTCGGCC TCTTCGATCACCAGCAACGGCGGCAGGTCTTTGTCAAGTGCACACTTCACCCCAGTGACCAGCTGGCCTT GGAGGGCCCTCTGTCTCGAGTAAAGTCCCTCAAGAAGTCTCTACGTCAGTCATTCCGTCGGATGCGCCGC AGCAGAGTGTCCAGTCATAAACGGCGGCCTGGTGGCCCCACAGGCGAGGCGCAGGCCCAGGCTGTGAATA TCAAGGCTGAGCGGACGGGCCTGCAGAACATGGAGTTGGCGCCCGTGCAACGGAAGATTGAGGCCCGCTC CGCAGAGGACTCCTTCACCGGCTTTGTCCGGACCCTCTATTTCGCTGATACCTACCTGAGGGACAGCTCC CGCCACTGTCCCTCCCTGTGGGCTGGCACCAACGGAGGCACCGTCTACGCCTTTTCCCTGCGCGTGCCTC CTGCAGAAAGAAGAACAGATGAACCTGTCCGGGCAGAGCAGGCCAAGGAGATCCAGCTGATGCATCGCGC GCCTGTGGTGGGCATCCTGGTGCTCGACGGACACAACGTGCCCCTCCCCGAGCCCCTGGAAGTCGCCCAT GACCTGTCGAAGAGCCCAGACATGCAAGGGAGCCACCAATTGCTTGTGGTGTCGGAGGAGCAATTCAAGG TATTCACACTGCCCAAGGTGAGCGCCAAACTGAAGCTGAAGCTGACGGCCCTGGAGGGCTCACGGGTGCG GCGAGTCGGTGTCGCCCACTTTGGCAGCTGCAGGGCTGAGGACTATGGGGAGCACCACCTGGCAGTGCTC ACCAACTTGGGTGACATCCAGGTGGTCTCAATGCCCCTGCTCAAGCCCCAGGTGCGGTACAGCTGCATCC GCCGGGAGGACGTCAGCGGCATAGCTTCCTGTGTCTTCACCAAATATGGCCAAGGTTTCTATCTGATATC ACCTTCGGAGTTCGAGCGCTTTTCTCTCTCCACCAAGTGGCTGGTTGAGCCCCGGTGTTTGGTGGATTCA ACCAAAGCCAAGAAGCACAACCGGCCCAGCAATGGCAATGGCACGGGCCCCAAAATGACCTCCTCAGGCC ACGTCAGGAACTCAAAGAGCCAAAGTGATGGAGATGAGAAGAAGCCGGGCCCAGTGATGGAGCACGCGCT GCTCAATGACGCCTGGGTCCTAAAGGAAATCCAGAGCACGCTGGAGGGGGACCGGAGGAGCTATGGCAAT TGGCATCCTCACCGCGTGGCTGTGGGTTGCAGGCTCAGCAATGGAGAAGCAGAATGACTGGGACAGCACC CAGGCTGTGGGATGATCACACACTACTGAGGGCCTTGCCAGCCCAAGCCGGACCAGGGGCTGCCAGTGCC CTGGCCACCTTCCTGTTGCTGCTCCTGGCCTCAGGAGAGGGGAGCTCCCACCGGGGCTGCCCCTCCTGGG CCCTGTCTGTCTGTCTGTCCTGACCCTTTTACCAATGTTGCACAGTTTTTATTTCCACCCTCTTTTGTAG TGGGCTGGGTTTTAAATTATAAATTTTAACCACCTCAAGTAAAACTGAGTTTTTAATAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584689 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584689 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7302 same embryo
	EMAGE:7301 same embryo
	EMAGE:7300 same embryo
	EMAGE:7298 same embryo
	EMAGE:7303 same embryo
	EurExpress:euxassay_004409 same experiment
	MGI:4825942 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source