EMAGE mouse gene expression spatial database

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EMAGE:7395

Pkia protein kinase inhibitor, alpha ( MGI:104747)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004656. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004656_01	euxassay_004656_02	euxassay_004656_03	euxassay_004656_04

euxassay_004656_05	euxassay_004656_06	euxassay_004656_07	euxassay_004656_08	euxassay_004656_09

euxassay_004656_10	euxassay_004656_11	euxassay_004656_12	euxassay_004656_13	euxassay_004656_14

euxassay_004656_15	euxassay_004656_16	euxassay_004656_17	euxassay_004656_18	euxassay_004656_19

euxassay_004656_20	euxassay_004656_21	euxassay_004656_22	euxassay_004656_23	euxassay_004656_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7395_wholemount_strong.wlz

7395_wholemount_moderate.wlz

7395_wholemount_weak.wlz

7395_wholemount_possible.wlz

7395_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7395_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 10 17

spinal cord

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 13 14 moderate expression: see section 09 15 16 17 weak expression: see section 10

not examined

regional

not examined expression: see section 01 02 03 04 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 weak expression: see section 10 15 16 17

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

tail mesenchyme

moderate

regional

moderate expression: see section 15 16 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7716
Entity Detected:	Pkia, protein kinase inhibitor, alpha ( MGI:104747)
Sequence:	sense strand is shown >T7716 TGCGAGCCCAGAGCCCAGCACACGCGGCCAGGCGGGCAGGACTGCAGCCAGGGGCAGCCGGGACGCGTCT CTCCATCCTGAAGTCCCTGCTATGTGGATATTTGTTAGCAATGACTGATGTGGAAACTACGTATGCAGAT TTCATTGCTTCAGGAAGAACAGGTAGAAGAAATGCAATACATGATATCCTGGTTTCCTCTGCAAGTGGCA ACAGCAATGAATTAGCCTTAAAACTAGCAGGCCTTGATATCAACAAGACAGAAGGTGAAGATGATGGACA GAGAAGCTCCACCGAACAAAGTGGAGAAGCCCAGGGAGAAGCAGCCAAGTCTGAAAGCTAACACTCCATT CTGACGGTCATCAACATCTGACAATGTGGCAAATATCCTGGCGATGCTCGGATGTATTCGCAGCCACGGG TGAAACGGAGGGGAAGAAAATGGCTGCGCTGCACAGCAAGACACTGTTGGTTGCCATGTCACAAGTGGGG ATGGAGCCTGTGTCTGCACACAGCCTTTTCCCCACCTCTGTCATTAGCAATGGTTGAAATCACCTGGGCT GTGCTTGGATCTCCTTCGTGTATGGGTCCTTCCACTTGACCGTATAATGAAATGCTTGTAGAGAGTAGCA CTGCCTAGCTGATGATTCCTGTGGCATCTGGCCCCTCATGATGTCAGAGGATTTAATTGTGTCTAATTGA AAAGAGTTGGTTGAACCCCAGAGTTTCAGTATCTCTGGCTGGAGTATTGACTCACAAAAAGAACCATCCA GAGAGCACAGTTAGCATTATGTCCCTGTGTGTGAGTGCTGTTACTTACGCCGTTTTGTATCGTACAGTAC ACCTGCTCTGCACTGCCCTTTGATTGCTTACATAAAACAAAATGATGTACATGACTGTGAATTTTTATAC CGCTCATTTTTTAAAGGGCTGCCTTTTTAATTTCCCATAGTGTTGTGGCGTGAACAGGGCAGAACCTTCT TTTGTAAAACACAACTTTCCTCTTGATAACTGCCGAGGGTTACATCCACTAGACTCACTGAGCCCACTTT GCTTTATTATACAGGCATGTGGAAATATCCATGAATGTGAATATTGCCTGAGTTCAGTCTTAGTATCTAC ACAGACCAGCGTTCTGTCTTTCAATATTGATTTTATTTCCAAATAAGTACTTTCAACTGATACTTTAATT TTTCATTATAAGAATTTTTTTAAGAATCAACAAAATAAACAAAGAATGCCAGATAATGCAAAGAGCTAGA AATGTTGTGCTGTGAGGATCACTTTTCTCACACTGCTGGGTGCCACTGTTTAACGCAGGAGGACAACTTG TGAAGGAAGTGTTTTCCTCAAAGCCTGTGGGCACCCATCTCAAAAGTAAAGCCCTTGTGCTGTGGCCCCT CTCAGCCCTTTCATACCCAAATGTTGTTACGCACTTTTTAATGTTTGTAAAATTTTACTAATAATTTGTG TGAATTGCATTCAGATATAAAAATTATCACCCAACTACACTTTTACTGATGCTGTTGATGGTCTACGCTG TGTTTTGACATCACAATTCTTGTATGGGGAGTTTCTGTGGCCTGTATAACCCTCTCTGGTCAGTATTATG AAACCAACTATCTTTGGTAATAAATGAGGATCCAGTTAGATGCCCAAGGTTCACCTGGTGCATCATAGGA GGCAAATAACAGATGAAAGAAGACTTTCTCAACAGAAGAGCCTCTAAGTGTCCTGGAGCACTAGCATAGC TACAGTTCCAGGTTAAGTAGGTAGAGGTGTTTCAACAGGCAGACCTGGGTACGGTAGGAACTCGACCTGA GTCTGTAGTTACAGACAAGGTAGACAAGGAAGTCTGCCTATATCTTCCCATAGAGTGCTTTCATTACTGA CTGGGAATATATGAATCTCATTTAGTCCCCAACTGGATGTTTTCCAGAAAAACAAGCACTGTTAAGTATG CCTTTCTGGATATAGAACAGTAAATACATTCAATATGAGAGGCCTTGCTTTGATCTAAGTCATTGGAGGC TAGCTAAAATTGAAACAAATCTACCTATTGATAGACTCAAAGTTGTGAACCTATGGCCTATTTGAGACAA TCTCCGATAATCTATTTACAACATCCAACAGTTTCAATGTCCTTGTTTAACTATGCTGATAGCTTTCTTG TGAATAGGAAGATCATTCTTAAAGTTGTGTAATTCCACACTGGTTTCACATGCATTTTGTAAAGTGTTGA ACCAGTGCTAAGACTGTGTGGAAGGGTTATACCCCTGTGTCATATACTATTACTAATGCATGCGGTGCCA TGCTTGTCTTTAAATATATAAGTAGTGCTAAATGCAGAAGTCATAGGGAGGTTCTGATTTCATTTGATTT CTTACTGCTACTTGGTGTCCTGTATTCAATCCTAGAGGGATTCTGTGGCTGGTAAGGGAAAAGAGATAAA CATTCTGAATTTGTCACATCACATAAATTTTAATTGGCAAAAGTGCATAGAATCTCCTGGTTTTCATTTA ATACTTATCATTTGCAAGTAAATAATACTACATAGGGAATGAATCTGTAAATGCAGATGGAAGTGTGGGC AGCCCAAGGAAAGGGGTGTTAGTTAACTACCCCAGCTAAGTCACTTCAGAACCAAATGTACCATTCAGTT TCATCTTATGCCCCTTTCACAAATTACAATGTGAACATGAATAAGGACTAATTTTTGCAAAACTAAAAAG CTAATTTGAGCCCATGTTTATCAACAGAATTTTGAATCCACATTTCATAAAATTTAAGCTAATTACACAG TGTGTTTCTCTGTATGGAAAACTATAGATAATACACTCAGTCAGCTTGCATTTTACAGAGAAAGAATTTA AGTTACTAAATAGACTAGCCTTTCTTGGGTTTGTACACATGTCTTTGGGACAAAGACACATGTGGTTGAG CCTACCTTTTTTTGTTGTTGTTGTTGTTTTAATACTCAATTATAGCTTATTTTGAATTTATTTAAGACAT GTAAACTGTAAATTTACATGCTATGTTCATGCTATGTATGTGAACAAATATTCTTGCTTTTTATTTAATA ACCTAGAAACACAAAATTTTCCATTTTAACACATTAACAATGAATGAGAAGCTGTAGAATTTGTTGAGCT TAACTTTTTTAAACTAAGAACATTTTATGTTAGTACATATTCATCCTGAATTCACAGAAATGCTTTCTGC TCTATAACAGAAAATCATGACCTTTATTTTTCAGGAAATACTGACATAGTAGCCCACTGTTCACACCCTA CCACTGAGTCTTCCTTAAATGTGACATCAAGGATCCAATGTAGAATAAAATGACATTTTTATCTCTTCTC AGAGGAATACGAAAAAAATGTAACTGTTGGGCAGCAAACTTCTAAATGAATTCTGCAGTAGTAAGGGAAA AAAATGTATAGTCATTAAATTAAACGAAGAAAGGATCCATAATTGATTGTGTCTTAGAAGCCTGGAAATG TTCTTTCACTGACTTGTGGCAGCCTGGGAAGTCATCTGTGAGCTTCTATGACTTTTGTATTTAGCAATGT TGCATGCTCACATAACTGATATTAAAAGCAGCACTTGTTTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6308831 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6308831 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7398 same embryo
	EMAGE:7394 same embryo
	EMAGE:7397 same embryo
	EMAGE:7396 same embryo
	EMAGE:7399 same embryo
	EurExpress:euxassay_004656 same experiment
	MGI:4827230 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source