EMAGE mouse gene expression spatial database

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EMAGE:7454

Ankrd32 ankyrin repeat domain 32 ( MGI:2145448)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004579. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004579_01	euxassay_004579_02	euxassay_004579_03	euxassay_004579_04

euxassay_004579_05	euxassay_004579_06	euxassay_004579_07	euxassay_004579_08	euxassay_004579_09

euxassay_004579_10	euxassay_004579_11	euxassay_004579_12	euxassay_004579_13	euxassay_004579_14

euxassay_004579_15	euxassay_004579_16	euxassay_004579_17	euxassay_004579_18	euxassay_004579_19

euxassay_004579_20	euxassay_004579_21	euxassay_004579_22	euxassay_004579_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7454_wholemount_strong.wlz

7454_wholemount_moderate.wlz

7454_wholemount_weak.wlz

7454_wholemount_possible.wlz

7454_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7454_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 19 20 21 22 23 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 07 08 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7664
Entity Detected:	Ankrd32, ankyrin repeat domain 32 ( MGI:2145448)
Sequence:	sense strand is shown >T7664 AAGAGGTAATCCGAGCTTTCCTCTTTGCAAGCTCATTATATCCCTCCTGTGTGCCTTCTCCATGCCATTC TAGAGAATGTTCTGCAGGATAAAATAGACACATTTTCTGGTCGATACTTTCATATATTATCAGCTCTTCT TCATCTGCATCCTCCTTGGAAGTCACCAGCCATGTTAAAATACTACTTAGAGCTATTTCAGTGTCCAACT TGTATGAAAGGAGCCTGGGATTTCACGGAGGTGCTCATCAGGTCATGTCTTTTCAATGAAGATTTTTGCC ATCAAATATCAGAAAATATTAGTACTAAGGTGGTCAATCTCACATTGCTCAAGTTTTTCTTTAATTTACT TGAAGGTGAAGTTCGGCATTTGAGTCAAAAGTTATGTGACTGGTCAGATTCTCAGAGTCTAAAGGTAACA GAAAAGGCAATTCTTCATGAAATTTTTTGGTCAGGAAGTGAAACTTCAGGACTTTTGACAAAACCAGTAA ATATGCTTTTGGAATGGACTATATATTCTCACAAGGAAAAATGCAAGTCTAATGATGTCTTCAAACATGA ACTATCTTACTTACTGACTGGAATTCTTGGTGCAGCAGTAGATTACTGGATCTTCCTTGGTATTCAGATG GGTAGAAATGTGATCCGACATATGTCTGATGACTTAGGAAGTTATATTTCCCTTTCCTGTGATGACTTTT CTTCAAAAGAACTAGAGATCTTCATTTGTTCCTTTTCATCTTCCTGGCTTCAAATGTTTGTTGCAGAAGC AATTTTTAAAAAGCTGTGCTTACAGGGCCCCACTAGTACGTGCACTGAGCCACTCTCTCTTCAGAAAATA ATAGATTCCTATTTGCCAATTTTGGGAAAAATGGACATACATGGGGCTGGAAAGATGCAGAGTCCAAAAA AACTCTGCCAGCGGCCTTGCCTAGAGTCTCAAAGAGCATTACTAATGCTGAATGGTGCAAAACGGAAACA AGCAGAAGGTCGGCCAGAGCTCCTAGAGTTAAACCGTGCTAAATGTTCCTCATCATTGAAAAAATTGAAA AAGAAGTCAGAGGAATTGTCATGTTCCAAGGAGAATTGCCCATCTTTGGTTACAAAGATGAATTTTCATA AAACTAATCTGAAAGGGGAAACAGCTCTGCATAGAGTTTGCATAAAAAACCAAGTGGAGAAATTGATTAT CCTTCTGTCTTTGCCAGGAATAGACATCAATGTTAAAGACAATGCTGGCTGGACGCCTTTGCATGAAGCC TGCAACTATGGCAACACGGAGTGTGTCCAGGAAATTCTGCAGCGTTGTCCAGAGGTGGATCTGCTCACTC AAGTGGACGGGGTGACGCCTTTGCATGATGCGCTGTCAAACGGACATGTGGAGATTGGCAAGCTGCTTCT ACAGCGTGGGGGCCCAGAACTTTTACAGCAGAGGAACTCTAAGGGGGAGTTGCCCTTGGATTATGTGCTG TCCCCTAAGGACAAGGAAGAACTATTTGCCATTACAAATATAGACGATACAGTGGACAACTTTCATGCTA AGACACAGAAACATTTTTACCACCAACAGCTTGAATTTGGTTCGTTTTTACTTAGTAGGATGTTGATTAA TTTTTGTTCAATATTTGATTTGTCCTCAGAATTTATTCTAGCTTTCAAAGGGTTAGGTCATCTAAATGAG TTGCTTATGGCATGCAATAGTGATACAGAAGCCAGCAATGCACATACTGACTGGTTATTGGATGTTTATG CTAGGAATATAAAGACATTAAAGAAGCTCCCAAGTGTTCTTAAAGAGCTGCCTGAGAATCTAAATGTATG TCCTGGAGTGCATACTGAGGCCTTACTGGTGACACTGAAAATGATGTGTCAGTCAATCACAGAGCTGTCA TGATGCCAGAAAGTATGAATCAGATGTCTGAACGTGCAACATGCTTTGTCATTTAGTATATCCATAGCTT ATGGAAAAAATATTAATTTCAGTTACTCACTGACAGCAAAGTTTCATAGCACTTATAAAACATCTACAGA TCTAAAATATGGGCTCAGGATTTAACTCAGAAACATACAATAAGAAAATGAAGTAGTTTTGTTCGGTATA AATGGTTTCACTGATTTTTAAAAAACAGATTTGTTCTTCAAATATTTATTTATATTATATATATAGAAAA TTAATTTAAATATTTTTTCAAGTCAAAATGTCCTTTCCTCTGAGTGCTACAGGTCTGAAAATCATGGCAA GGATATTCATTCAAATGTGCTTCTATATACATGTAAAAGTTGTTTGTTCTTTATATTTAAGATGCATTTG CTTTTTTGTGTTTCTAAAATAAACCTTTCTAGGACTTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30458612 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30458612 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7455 same embryo
	EMAGE:7459 same embryo
	EMAGE:7457 same embryo
	EMAGE:7458 same embryo
	EMAGE:7456 same embryo
	EurExpress:euxassay_004579 same experiment
	MGI:4823133 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source