EMAGE mouse gene expression spatial database

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EMAGE:7455

Ccdc125 coiled-coil domain containing 125 ( MGI:1923291)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004576. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004576_01	euxassay_004576_02	euxassay_004576_03	euxassay_004576_04

euxassay_004576_05	euxassay_004576_06	euxassay_004576_07	euxassay_004576_08	euxassay_004576_09

euxassay_004576_10	euxassay_004576_11	euxassay_004576_12	euxassay_004576_13	euxassay_004576_14

euxassay_004576_15	euxassay_004576_16	euxassay_004576_17	euxassay_004576_18	euxassay_004576_19

euxassay_004576_20	euxassay_004576_21	euxassay_004576_22	euxassay_004576_23	euxassay_004576_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7455_wholemount_strong.wlz

7455_wholemount_moderate.wlz

7455_wholemount_weak.wlz

7455_wholemount_possible.wlz

7455_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7455_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7662
Entity Detected:	Ccdc125, coiled-coil domain containing 125 ( MGI:1923291)
Sequence:	sense strand is shown >T7662 AAGAGGTAATCCCCCGGACCTGGCGATCCTTTCGGGTCCCCTGCGGTGGTCGTGCAGTCACGTGGCCCGA GGACGCGTGGCGGAGTGGGCATTTGGCTAATGAGCAAAGTGCCGAGGTCATCGAGCGAGGCAGAGGACAT CTGGGAAACAGAGGATGACATGACCGAAGGTGACCTAGGCTATGGCCTCGGAAGAAAACCCGGTGGTATT TATGAAGTTCCGTGTTCGATTACATCTAAGAAAAGGTCAGATGGAAAGAACTCAAGCCCTCCTCCGTTTC CAAGAAAGGGAGAAGAAAGAAGCGAAACCAGTTTCCAGTATTCCAGGAGGAAGGGCTTTCAAGATACAAG CGCTGAGGGATACCGAGCATCCAGACTGAGCAGCACAGATTCTAACTCAGAATTGTCGGATGAGCAGTTA AGGCGACGTCTTCATGAAGCCTTAGAGGACGTAGAGATTTTAAAAACGGAACTTGAAGCGTCTCAAAGAC AACTTGAAGGTAAAGAGGAAGCATTGAAAATCCTCCAAAGCATGGCAATGCTTGGCAAAGCCACAAGCCA CACACAGACAATGCTTCAAAAAACTATAGAACAAAAGAGATCTCTGGAGAAGGAAATAAATGCCTTGCAG TGGGAAATGGAATTTGATCAGGATAGATTTAAAAATATAGAAGAATCTTGGATCCAGAAATGTGACAGGC TAAACTGTGACAATGCAGTCCTCAGAGAGAATCTGAAGTTGAGAACAGAGGAAATAAAGATGCTAAAGTC TAAGAATGCTGTTTTGAATCAGCGGTACTTGGAGGCCCTCGCCATGCTTGATATCAAGGAGCAGAAGATG GGTCAGGAGGAGAGTGGCTTTACAGATGTATCAGGTCTCGAGCTTGCAGTCCTTGGAGCCTGCCTGTGTC ATGGTCCTGGAGGGAGCCCCTGTTCTTGTGCCAAAATGGCAGCATCCACTCGGAAACTGGTTCTTCAGCT CAGACATGAGTTGGAAACTCTGCAGAAGAGTAAGGAAGAGGCGCACATAACGGCAGATGCATTCAGGATT GCTTTTGAGCAACAGTTAATGAGGAAAAATGAGCAGGCACTGAGACTGGCTGGAGGGGACCTGTGTAAAA GAGCGGCAACGTGGACCAACAGACAACACCAGGCAGACGATGGATATCCGGCACAAAGGAGAAAGAAGAC CTTAGGGCAAAGATTACTGGGGATACTCCCTTCGGAAAACAGCTCCAAGGGCGCTGAAGACCAAGACAAT ATGCAAGAGGTCTTTAAGATGCTGGTAGATTTGTTGAATGACAAAGAAGAAGCCCTTGCACATCAGAGAA AGGTTAGTTACATGCTCGCTCGGGCGCTGGAAGACAAAGACACGGCCTCAGAAAGGAATAAAGAAAAAAT CCCCATGAGCCAGACCTTCCCATTCAAAACGGCCTGGCACGACGCTTCAGAGCTCTGTGGTCTGCGTGAT CCAGTACAGTCAAACCATGTTTCTGAACCCGTGGCTTGCATCTGTTCAATACAGCATCCTCCAAAAGTTT CAGACTGCCCAAGAACTCTTAAAAGATCCTGTTCTTTGCCATCAACTTTATTTTACAAGTAAACCACTTG AAGTTAGAATTAAGAGATTCTTAAAAGTCAGATGTTGGAGCTGGAAACGGAGCTCAGAGGTAGACATTTG GTTAGCGTGCCTGAGGCCTGGGGTCAGTCCATGGTGGCGCACGCCTTTAACCCTGGCACTCAGGAAAGCA GAGGCAGGCGATCTCTGACTTTGAGGCCAGTCTGGTCTACAGAGTGAGTTCCAGCACAAATGCTTCATAA GGCAATCCTGTCGTGGGAGAATACAAAACGGGCCTTGGGTCTGATGTTCAGCACTGCAAAGACTGTCTGG AAGCTCAGGCCTGGAAATCCCAGCACGTGGGAGGCTGAGGCAGGACAACTACTGTAAGTTTGAGGGATAA CTAGATTACATTAAGACTGTTGCTAAAGCCGGGCGTGGTAGCACACACACACACACTCAGGAGGCAGAGG CAGGCGGATTTCTAAGTTCGAGACCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAG AGAAACCCTGTCTCGAAAAAATAAAATAAAATTAAAAAAAAAAAAAAAGACTGTTGCCAAAACAGAAGAA CAACTATATAAATGTCAATATGAAGACGCTGTAGGCTTTCAAGGTGTTTTAAAAATTTATTAGCATAGTA ATTTACAAAGTTGTAAGTTTCATTATGATATCTTAATACACATATTAATACATGTGTTTTGATGACATTC CTCCCTTTTAATTTTAGGATGCAGCTTAAGCAAGCGAACCCTTATCACATGTCCTCCAAGAAATGTCTTA ATATTTGTGTTGACATTTTTTATAAACTAATTTCCATATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30726150 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30726150 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7454 same embryo
	EMAGE:7459 same embryo
	EMAGE:7457 same embryo
	EMAGE:7458 same embryo
	EMAGE:7456 same embryo
	EurExpress:euxassay_004576 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source