EMAGE mouse gene expression spatial database

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EMAGE:7456

Soat1 sterol O-acyltransferase 1 ( MGI:104665)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004596. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004596_01	euxassay_004596_02	euxassay_004596_03	euxassay_004596_04

euxassay_004596_05	euxassay_004596_06	euxassay_004596_07	euxassay_004596_08	euxassay_004596_09

euxassay_004596_10	euxassay_004596_11	euxassay_004596_12	euxassay_004596_13	euxassay_004596_14

euxassay_004596_15	euxassay_004596_16	euxassay_004596_17	euxassay_004596_18	euxassay_004596_19

euxassay_004596_20	euxassay_004596_21	euxassay_004596_22	euxassay_004596_23	euxassay_004596_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7456_wholemount_strong.wlz

7456_wholemount_moderate.wlz

7456_wholemount_weak.wlz

7456_wholemount_possible.wlz

7456_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7456_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19

viscerocranium

moderate

regional

Expression in the turbinate bone.

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10

midgut

moderate

regional

moderate expression: see section 15 16

midgut loop

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

kidney calyx

moderate

regional

moderate expression: see section 06 07 08 09 10 11 16 17 18 19 weak expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7665
Entity Detected:	Soat1, sterol O-acyltransferase 1 ( MGI:104665)
Sequence:	sense strand is shown >T7665 AAGAGGTAATCCGGGAGAAGAGAAGATGTCACTAAGAAACCGGCTGTCAAAATCTGGGGAGAATCCTGAG CAAGATGAAGCCCAGAAAAATTTCATGGACACATACAGAAATGGTCACATTACCATGAAACAATTGATAG CCAAGAAGAGGCTGTTGGCAGCAGAGGCGGAGGAGCTGAAGCCACTATTTATGAAGGAAGTTGGGTGCCA CTTCGATGACTTTGTGACCAACCTCATTGAAAAGTCCGCATCGCTGGACAATGGTGGGTGTGCACTCACA ACCTTCTCCATTCTTGAAGAAATGAAAAAGAACCACAGAGCCAAAGATCTGAGAGCACCTCCAGAACAAG GGAAGATTTTTATTTCAAGGCAGTCTCTTTTAGACGAGCTGTTTGAAGTGGACCACATCAGAACAATTTA CCACATGTTCATCGCACTCCTCATCCTATTCGTCCTCAGCACGATCGTCGTGGACTACATCGATGAAGGA AGGCTGGTACTTGAGTTCAATCTCCTGGCCTATGCTTTTGGCAAATTTCCTACTGTTATTTGGACATGGT GGGCCATGTTCCTGTCTACACTGTCAATCCCCTATTTCCTGTTCCAGCGATGGGCCCACGGTTACAGCAA GAGTTCTCACCCATTGATCTATTCCCTTGTCCATGGCTTGCTCTTCTTAGTCTTTCAACTTGGAGTTCTA GGTTTTGTGCCAACATACGTTGTCTTAGCATACACACTGCCACCAGCCTCCCGGTTCATTCTGATACTGG AACAGATTCGTTTGATAATGAAGGCTCACTCATTTGTCAGAGAGAATATCCCACGAGTACTAAATGCAGC CAAGGAGAAATCAAGCAAAGATCCACTACCCACAGTCAACCAGTACCTGTACTTCCTGTTTGCGCCTACA CTTATTTACCGAGACAACTACCCAAGGACTCCTACTGTAAGATGGGGTTATGTCGCTATGCAGTTTTTAC AGGTGTTTGGCTGCCTGTTTTATGTGTACTACATCTTTGAGAGACTCTGTGCCCCACTATTCCGGAATAT CAAACAGGAGCCCTTCAGTGCTCGTGTCCTGGTCCTGTGTGTGTTTAACTCCATCTTGCCAGGTGTCTTG ATACTGTTCCTTTCGTTCTTTGCCTTTTTGCACTGCTGGCTCAATGCCTTTGCTGAGATGTTACGCTTTG GTGACAGGATGTTTTATAAGGACTGGTGGAACTCTACATCATACTCCAACTACTACAGGACCTGGAACGT GGTGGTGCACGACTGGCTCTACTACTATGTTTACAAAGACCTGCTCTGGTTTTTCTCGAAGAGGTTCAAA TCTGCCGCCATGCTGGCCGTCTTCGCCCTGTCGGCTGTGGTGCACGAGTATGCCCTCGCCATCTGCCTGA GTTACTTCTACCCGGTGCTCTTCGTGCTCTTCATGTTCTTTGGAATGGCTTTTAACTTCATTGTTAACGA CAGTCGGAAAAGGCCAATCTGGAACATCATGGTTTGGGCTTCTCTGTTCCTGGGCTATGGACTCATTCTG TGCTTTTATTCTCAAGAGTGGTATGCCCGCCAGCACTGTCCTCTGAAGAACCCTACATTTCTGGATTATG TCCGGCCGCGGACCTGGACTTGTCGGTACGTGTTTTAGAAGCCCTGGCTCTGCCTCCTTGCTTGCCATTG AAGATTGGATATTCTTCCTGATTCCGAGCCAGCCGTCTTCAGCGGTTACTGAATCCCCACACTGCGGTGT TTGGGGCTCTCTATCCTTTCTGATGATTCACCTGGAGTTGGGCTGTGGGAAGCCCACTTCGTGATTTTTT TGTTTTGTTTTGTTTTGTTCCCTTTGGGGGAGAAAGGAGACTGACAGTTGAGATAATGGCAGATTTTTGC AGGGTTATCTCAGGTCAAGCCTTGGTAGTTTTATCTGTTTATTTCCTTGATTCCATCCAACCAGAGACTA AACATATCTTGACCATGAGTTAGCCAAAACACTTAGGAAGACATCATTTCTGTATCTCTGAGCATAGAAG TTTTGTTCATGCACATTATCAAGAGGTATGCTAGTGCAATTGAGAAGAAAGAAATTTTGTTATTTTTGGT AGAGAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30446741 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30446741 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7455 same embryo
	EMAGE:7454 same embryo
	EMAGE:7459 same embryo
	EMAGE:7457 same embryo
	EMAGE:7458 same embryo
	EurExpress:euxassay_004596 same experiment
	MGI:4828371 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source