EMAGE mouse gene expression spatial database

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EMAGE:7579

Stat1 signal transducer and activator of transcription 1 ( MGI:103063)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004660. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004660_01	euxassay_004660_02	euxassay_004660_03	euxassay_004660_04

euxassay_004660_05	euxassay_004660_06	euxassay_004660_07	euxassay_004660_08	euxassay_004660_09

euxassay_004660_10	euxassay_004660_11	euxassay_004660_12	euxassay_004660_13	euxassay_004660_14

euxassay_004660_15	euxassay_004660_16	euxassay_004660_17	euxassay_004660_18	euxassay_004660_19

euxassay_004660_20	euxassay_004660_21	euxassay_004660_22	euxassay_004660_23	euxassay_004660_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7579_wholemount_strong.wlz

7579_wholemount_moderate.wlz

7579_wholemount_weak.wlz

7579_wholemount_possible.wlz

7579_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7579_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 03 04 05 06 20 21 weak expression: see section 07

axial muscle

moderate

regional

moderate expression: see section 22

pectoral girdle and thoracic body wall muscle

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

thymus primordium

strong

homogeneous

strong expression: see section 11 12 13 14 15

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 16 17

pons mantle layer

weak

regional

weak expression: see section 08 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6476
Entity Detected:	Stat1, signal transducer and activator of transcription 1 ( MGI:103063)
Sequence:	sense strand is shown >T6476 GTCAAAGCTCCCTGGAGACCTCCGGGACCGCGCCCCTCAGACCCACTTGGGACACTGCTGAGCGGCGCAG AGAGATTTGCCCAGACTCGAGCTCCTGCGTGCAGTGAGTGAGTGAGAGCCAGTCGTTTCAGCTCTGCTCC ATACCCTGAGCCGGCCCCACGCCGCCGCGCATGCAACTGGCATATAACTTGCTGTGTGTGGTGATTGCTT GTGTTGAATCCCGAACCTGCCCCCGGAGACAGCCCAGTAAGTCTACGTGGGAACGGAAGCATTTGGAATC TCAAGGATGTCACAGTGGTTCGAGCTTCAGCAGCTGGACTCCAAGTTCCTGGAGCAGGTCCACCAGCTGT ACGATGACAGTTTCCCCATGGAAATCAGACAGTACCTGGCCCAGTGGCTGGAAAAGCAAGACTGGGAGCA CGCTGCCTATGATGTCTCGTTTGCGACCATCCGCTTCCACGACCTCCTCTCGCAGCTGGACGACCAGTAC AGCCGCTTTTCTCTGGAGAATAATTTCTTGTTGCAGCACAACATACGGAAAAGCAAGCGTAATCTCCAGG ATAACTTCCAAGAAGATCCCGTACAGATGTCCATGATCATCTACAACTGTCTGAAGGAAGAAAGGAAGAT TTTGGAAAATGCCCAAAGATTTAATCAGGCCCAGGAGGGAAATATTCAGAACACTGTGATGTTAGATAAA CAGAAGGAGCTGGACAGTAAAGTCAGAAATGTGAAGGATCAAGTCATGTGCATAGAGCAGGAAATCAAGA CCCTAGAAGAATTACAAGATGAATATGACTTTAAATGCAAAACCTCTCAGAACAGAGAAGGTGAAGCCAA TGGTGTGGCGAAGAGCGACCAAAAACAGGAACAGCTGCTGCTCCACAAGATGTTTTTAATGCTTGACAAT AAGAGAAAGGAGATAATTCACAAAATCAGAGAGTTGCTGAATTCCATCGAGCTCACTCAGAACACTCTGA TTAATGACGAGCTCGTGGAGTGGAAGCGAAGGCAGCAGAGCGCCTGCATCGGGGGACCGCCCAACGCCTG CCTGGATCAGCTGCAAAGCTGGTTCACCATTGTTGCAGAGACCCTGCAGCAGATCCGTCAGCAGCTTAAA AAGCTGGAGGAGTTGGAACAGAAATTCACCTATGAGCCCGACCCTATTACAAAAAACAAGCAGGTGTTGT CAGATCGAACCTTCCTCCTCTTCCAGCAGCTCATTCAGAGCTCCTTCGTGGTAGAACGACAGCCGTGCAT GCCCACTCACCCGCAGAGGCCCCTGGTCTTGAAGACTGGGGTACAGTTCACTGTCAAGCTGAGACTGTTG GTGAAATTGCAAGAGCTGAACTATAACTTGAAAGTGAAAGTCTCATTTGACAAAGATGTGAACGAGAAAA ACACAGTAAAAGGATTTCGGAAGTTCAACATCTTGGGTACGCACACAAAAGTGATGAACATGGAAGAATC CACCAACGGAAGTCTGGCAGCTGAGTTCCGACACCTGCAACTGAAGGAACAGAAAAACGCTGGGAACAGA ACTAATGAGGGGCCTCTCATTGTCACCGAAGAACTTCACTCTCTTAGCTTTGAAACCCAGTTGTGCCAGC CAGGCTTGGTGATTGACCTGGAGACCACCTCTCTTCCTGTCGTGGTGATCTCCAACGTCAGCCAGCTCCC CAGTGGCTGGGCGTCTATCCTGTGGTACAACATGCTGGTGACAGAGCCCAGGAATCTCTCCTTCTTCCTG AATCCCCCGTGCGCGTGGTGGTCCCAGCTCTCAGAGGTGTTGAGTTGGCAGTTTTCATCAGTCACCAAGA GAGGTCTGAACGCAGACCAGCTGAGCATGCTGGGAGAGAAGCTGCTGGGCCCTAATGCTGGCCCTGATGG TCTTATTCCATGGACAAGGTTTTGTAAGGAAAATATTAATGATAAAAATTTCTCCTTCTGGCCTTGGATT GACACCATCCTAGAGCTCATTAAGAAGCACCTGCTGTGCCTCTGGAATGATGGGTGCATTATGGGCTTCA TCAGCAAGGAGCGAGAACGCGCTCTGCTCAAGGACCAGCAGCCAGGGACGTTCCTGCTTAGGTTCAGTGA GAGCTCCCGGGAAGGGGCCATCACATTCACATGGGTGGAACGGTCCCAGAACGGAGGTGAACCTGACTTC CATGCTGTGGAGCCCTACACGAAAAAAGAGCTTTCAGCTGTTACTTTCCCAGATATTATTCGCAACTACA AAGTCATGGCTGCCGAGAACATACCAGAGAATCCCCTGAAGTATCTGTACCCCAATATTGACAAAGACCA CGCCTTTGGGAAGTATTATTCCAGACCAAAGGAAGCACCAGAACCGATGGAGCTTGACGACCCTAAGCGA ACTGGATACATCAAGACTGAGTTGATTTCTGTGTCTGAAGTGTAAGTGAGCACAGAAGACTGGCTTACTG GCTTGTTCAGGACCGCAGGCCAGCCCCGTCCCTGGCTGCGGTCCATTGATTGCACTGGCTCATGGCTCAT GGCTTGCTTTCCCAATGTAACTGATGTTGCCACCACAGTTGGACATTCTGGAAAGTTTGTAACTAGTCCT GGTGTCAATGCTTACTGGCATTTTATTGCCATGAGATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3587831 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3587831 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7583 same embryo
	EMAGE:7582 same embryo
	EMAGE:7581 same embryo
	EMAGE:7580 same embryo
	EMAGE:7585 same embryo
	EMAGE:7584 same embryo
	EurExpress:euxassay_004660 same experiment
	MGI:4828484 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source