EMAGE mouse gene expression spatial database

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EMAGE:7582

Clk3 CDC-like kinase 3 ( MGI:1098670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004643. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004643_01	euxassay_004643_02	euxassay_004643_03	euxassay_004643_04

euxassay_004643_05	euxassay_004643_06	euxassay_004643_07	euxassay_004643_08	euxassay_004643_09

euxassay_004643_10	euxassay_004643_11	euxassay_004643_12	euxassay_004643_13	euxassay_004643_14

euxassay_004643_15	euxassay_004643_16	euxassay_004643_17	euxassay_004643_18	euxassay_004643_19

euxassay_004643_20	euxassay_004643_21	euxassay_004643_22	euxassay_004643_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7582_wholemount_strong.wlz

7582_wholemount_moderate.wlz

7582_wholemount_weak.wlz

7582_wholemount_possible.wlz

7582_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7582_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

trigeminal v ganglion

weak

graded

weak expression: see section 05 06 07 08 17 18 19 20 21 22

dorsal root ganglion

weak

regional

weak expression: see section 08 09 12 13 14 15 16 17

not examined

regional

not examined expression: see section 01 02 03 04 22 23

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 22 23

mandible

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 15 16 17 18 19 20 21 22

maxilla

moderate

regional

moderate expression: see section 06 07 08 16 17 18 19 20

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7724
Entity Detected:	Clk3, CDC-like kinase 3 ( MGI:1098670)
Sequence:	sense strand is shown >T7724 GGCCTAGCCGCAGCCGGAGCCTGGGAGACGATGCATCACTGTAAGCGATACCGTTCCCCAGAGCCAGACC CATACCTGAGCTACCGCTGGAAGAGGAGGCGGTCTTACAGTCGGGAGCATGAAGGTCGACTACGATACCC ATCCCGAAGGGAGCCTCCCCCACGGAGATCACGGTCCAGAAGCCATGATCGTATACCCTACCAGCGGAGG TACCGGGAACACCGTGACAGTGATACGTATCGGTGTGAAGAGCGGAGCCCATCTTTTGGAGAGGACTGCT ATGGGTCTTCACGTTCTCGACATCGGAGACGGTCACGAGAGAGGGCGCCGTACCGTACCCGCAAGCATGC CCACCACTGTCACAAACGCCGTACCAGGTCTTGTAGCAGTGCTTCCTCGAGAAGCCAACAGAGCAGTAAG CGCAGCAGCCGGAGTGTGGAAGATGACAAGGAGGGCCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAG AGCGATATGAGATCGTGGGGAACCTGGGTGAAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGC CAGAGGGAAGTCACAGGTTGCCCTGAAGATCATCCGTAATGTGGGCAAGTATCGGGAAGCTGCTCGTCTA GAAATTAATGTTCTCAAGAAAATCAAGGAGAAAGACAAGGAAAATAAGTTCCTTTGTGTCCTGATGTCTG ACTGGTTCAACTTCCATGGTCATATGTGCATCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCT GAAGGAGAACAACTTCCAGCCTTACCCCCTACCACATGTCCGGCACATGGCCTACCAGCTCTGTCATGCC CTTAGATTTCTACACGAGAACCAGCTGACCCACACAGACTTGAAGCCAGAGAACATCTTGTTTGTGAATT CTGAGTTTGAAACCCTCTACAATGAGCACAAGAGCTGCGAGGAGAAGTCAGTGAAGAACACCAGCATCCG AGTGGCAGACTTTGGCAGTGCCACGTTTGACCATGAACATCACACCACCATTGTGGCCACCCGTCACTAC CGCCCACCTGAGGTGATCCTTGAGCTGGGCTGGGCACAGCCCTGTGATGTCTGGAGTATCGGCTGCATTC TCTTTGAGTACTACCGTGGCTTTACACTCTTCCAGACCCATGAAAATAGAGAACACTTGGTTATGATGGA GAAGATTCTAGGACCCATCCCATCACACATGATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGG GGCCTGGTTTGGGATGAGAACAGCTCTGATGGGCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTT ACATGCTGCAGGACTCCCTGGAGCATGTGCAGCTGTTTGACCTGATGAGGAGGATGTTAGAGTTCGACCC TGCTCAGCGCATCACATTGGCAGAAGCCTTGCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGG TCCTTCCACAGCAGCCGTAACCCCAGCAGATGACAGGTGCAGGCCAGCACACGAAGAGTTGGAGAGCTGG ACTGGGCTGCTGGCCCCTTTTCTCCAGCCTCTCCCACTGGCCTCAGAGCCAGAGCCACCGATGAACAGTG CAATGTGAAGGAAGGCAGGAGCCTGCAAGGGAAGGGGGAATGTGGTGCCCGGCTGCCAGAAAGCACAGAT TGGACCCAAGCTATTTATATGTTGTAAAGTTATAATAAAGTGTTTCTTACTGTTTGTAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309020 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309020 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7583 same embryo
	EMAGE:7581 same embryo
	EMAGE:7580 same embryo
	EMAGE:7585 same embryo
	EMAGE:7579 same embryo
	EMAGE:7584 same embryo
	EurExpress:euxassay_004643 same experiment
	MGI:4823930 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source