EMAGE mouse gene expression spatial database

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EMAGE:7590

Tpp2 tripeptidyl peptidase II ( MGI:102724)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004663. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004663_01	euxassay_004663_02	euxassay_004663_03	euxassay_004663_04

euxassay_004663_05	euxassay_004663_06	euxassay_004663_07	euxassay_004663_08	euxassay_004663_09

euxassay_004663_10	euxassay_004663_11	euxassay_004663_12	euxassay_004663_13	euxassay_004663_14

euxassay_004663_15	euxassay_004663_16	euxassay_004663_17	euxassay_004663_18	euxassay_004663_19

euxassay_004663_20	euxassay_004663_21	euxassay_004663_22	euxassay_004663_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7590_wholemount_strong.wlz

7590_wholemount_moderate.wlz

7590_wholemount_weak.wlz

7590_wholemount_possible.wlz

7590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7709
Entity Detected:	Tpp2, tripeptidyl peptidase II ( MGI:102724)
Sequence:	sense strand is shown >T7709 TCTGTCCTCCAGCTTGCGTCCATGGCCACCGCCGCGACCGAGGAGCCCTTTCCTTTCCACGGTCTTCTAC CAAAGAAGGAGACCGGGGCCTCCTCCTTCCTGTGCCGCTACCCGGAGTATGACGGGCGCGGGGTGCTCAT CGCCGTCCTGGACACAGGGGTCGATCCCGGGGCCCCGGGCATGCAGGTTACAACTGATGGAAAACCAAAA ATCATTGATATCATTGATACAACAGGAAGTGGTGATGTAAATACTGCTACAGAAGTAGAACCAAAAGATG GTGAAATTATTGGTCTTTCTGGAAGAGTGCTTAAGATTCCTGCAAACTGGACAAATCCTTTAGGAAAATA TCATATTGGCATTAAAAATGGTTATGACTTCTATCCAAAGGCTCTCAAGGAAAGGATACAGAAAGAACGG AAGGAAAAAATCTGGGATCCAATTCACAGAGTTGCACTTGCAGAAGCTTGTAGAAAACAAGAAGAATTTG ATATTGCCAACAATGGCTCTTCCCAAGCCAATAAACTAATCAAGGAAGAGTTGCAAAGTCAAGTGGAATT ACTTAATTCTTTTGAGAAAAAGTATAGTGATCCAGGCCCTGTATATGACTGCTTGGTGTGGCATGATGGT GAGACCTGGAGAGCCTGTGTTGATTCAAATGAGAATGGGGACTTGAGTAAATGTGCCGTATTGAGAAACT ACAAAGAGGCCCAAGAGTACAGTTCTTTTGGCACAGCTGAGATGCTGAATTACTCTGTGAACATTTATGA CGATGGGAACCTGCTCTCCATTGTGACCAGCGGAGGAGCTCATGGAACCCATGTAGCAAGTATAGCCGCA GGGCATTTTCCAGAAGAGCCTGAACGGAATGGAGTTGCTCCTGGTGCTCAAATTCTATCCATTAAGATTG GTGATACACGGCTAAGCACTATGGAAACAGGCACAGGCCTCATCAGAGCTATGATAGAAGTTATAAATCA TAAGTGTGATCTTGTCAACTACAGTTATGGAGAAGCAACTCATTGGCCAAATTCTGGGAGAATTTGTGAA GTAATTAATGAAGCAGTATGGAAACATAATACAATTTATGTTTCAAGTGCTGGAAATAATGGTCCATGCC TTTCTACAGTGGGTTGTCCAGGAGGAACTACATCCAGTGTGATAGGTGTTGGAGCTTATGTTTCCCCTGA TATGATGGTTGCAGAGTATTCACTGAGAGAGAAACTGCCTGCAAATCAATATACATGGTCTTCTAGAGGC CCAAGTGCTGATGGAGCCCTCGGTGTGAGCATCAGTGCACCAGGAGGTGCTATTGCTTCTGTGCCTAACT GGACATTGAGGGGGACTCAGCTAATGAATGGGACATCAATGTCTTCCCCCAATGCCTGTGGTGGCATTGC CCTGGTACTTTCAGGGCTGAAAGCAAATAATGTTGACTATACTGTACACTCAGTCAGAAGAGCTCTAGAA AATACTGCAATAAAAGCTGACAATATAGAAGTATTTGCCCAAGGACATGGAATTATTCAGGTTGACAAAG CTTATGACTACCTCATTCAAAATACATCATTTGCTAACAGATTAGGTTTTACAGTTACTGTTGGAAATAA CCGTGGTATCTACCTCCGAGATCCTGTCCAGGTGGCTGCTCCTTCAGATCATGGTGTTGGCATTGAGCCT GTATTTCCAGAGAACACAGAAAACTCTGAAAAAATATCCTTTCAGCTTCATTTAGCTTTAACTTCAAATT CATCTTGGGTTCAGTGTCCCAGCCATTTGGAACTCATGAATCAGTGTCGGCACATAAACATACGTGTGGA CCCCAGGGGCTTAAGAGAAGGGTTACATTATACAGAGGTATGTGGCTATGATATAGCATCCCCCAATGCA GGTCCTTTATTCAGAGTTCCAATCACTGCAGTTATAGCAGCAAAAGTAAATGAGTCATCACATTATGATC TAGCCTTTACAGATGTACATTTTAAACCTGGTCAGATTCGAAGACATTTTGTTGAGGTTCCTGAAGGGGC AACCTGGGCTGAAGTTACCGTGTGTTCATGTTCTTCTGAGGTATCGGCAAAATTTGTTCTTCATGCAGTA CAGCTTGTGAAGCAGAGAGCATATCGAAGTCATGAATTTTATAAGTTTTGTTCCCTTCCAGAAAAAGGAA CACTTATTGAAGCTTTTCCTGTTTTGGGCGGAAAAGCAATTGAATTTTGTATTGCTCGTTGGTGGGCAAG TCTTAGTGATGTCAATATTGATTATACCATATCATTCCATGGGATAGTGTGTACTGCACCACAGTTAAAC ATTCATGCATCTGAAGGAATCAATCGTTTTGATGTTCAGTCCTCTTTAAAGTATGAAGATCTGGCTCCTT GCATAACTTTGAAGAGCTGGGTGCAAACGCTACGCCCAGTAAATGCAAAAACCAGACCTTTAGGATCAAG AGATGTTTTGCCAAATAATCGCCAGCTTTATGAGATGGTCCTTACATACAGCTTTCATCAGCCCAAGAGC GGAGAAGTAACACCTAGTTGTCCACTCCTTTGTGAATTGTTATATGAGTCAGAATTTGACAGTCAGTTGT GGATTATTTTCGACCAGAACAAAAGACAGATGGGCTCAGGCGATGCCTATCCACATCAGTATTCTCTGAA ATTGGAGAAAGGAGATTATACAATTCGATTACAGATTCGTCATGAGCAAATCAGTGATTTGGATCGTCTC AAAGATCTTCCGTTTATTGTTTCACATAGGTTGTCTAATACCTTGAGCTTAGATATTCATGAAAATCATA GCCTTGCACTTCTAGGAAAGAAGAAATCAAGCAGCTTAACATTACCACCCAAATACAATCAGCCATTCTT TGTTACTTCCTTACCTGATGATAAAATACCTAAGGGGGCAGGACCCGGATGCTACCTTGCAGGCTCCTTG ACATTGTCAAAGACTGAGCTTGGAAAGAAAGCTGATGTGATCCCTGTTCATTACTATCTCATACCTCCAC CAACAAAGATTAAGAATGGCAGCAAAGATAAAGAAAAGGATTCAGAAAAAGAGAAAGACTTGAAAGAAGA GTTTACTGAAGCATTACGCGATCTCAAAATTCAGTGGATGACCAAGCTTGATTCTACTGACATTTACAAT GAATTGAAAGAAACATATCCTGCTTACCTTCCTTTGTATGTTGCACGTCTTCATCAACTGGATGCTGAAA AGGAACGAATGAAAAGACTTAATGAAATTGTTGATGCTGCCAATGCTGTTATTTCTCACATCGATCAAAC CGCTCTTGCAGTTTACATTGCAATGAAGACTGACCCCAGGCCTGATGCAGCTACTATAAAAAATGATATG GACAAGCAGAAATCCACCCTGATAGATGCCCTCTGCAGGAAAGGATGTGCCCTGGCAGATCACCTTCTTC ATACACAGCCTCACGACGGGGCAGCAGCTGGAGATGCTGAAGCAAAGGAAGAAGAAGGAGAAAGTACCAT GGAATCTCTATCGGAAACCTATTGGGAAACTACAAAGTGGACAGATCTTTTTGACACTAAGGTTTTGATA TTTGCATACAAGCATGCATTAGTAAATAAGATGTATGGGAGAGGCCTTAAGTTTGCAACCAAACTCGTAG AAGAAAAACCAACAAAAGAAAACTGGAAAAATTGTATTCAACTCATGAAATTACTTGGATGGACCCACTG TGCATCTTTTACTGAAAACTGGCTCCCCATCATGTATCCTCCTGATTATTGTGTATTCTAAAATAAGAAC CAAAACTTTACATTTCAAAACAGAAAATTTTATAGTGAATGGATATATGAAGAATTGTGTGGCATTTTTA GTCTGACTAATGCATGTTTTCATCTACCATTCAATACTGATCATTAAAACTATATGTATTTATCAGAGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6308147 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6308147 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7594 same embryo
	EMAGE:7593 same embryo
	EMAGE:7589 same embryo
	EMAGE:7592 same embryo
	EMAGE:7591 same embryo
	EurExpress:euxassay_004663 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source