EMAGE mouse gene expression spatial database

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EMAGE:7615

Usp19 ubiquitin specific peptidase 19 ( MGI:1918722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004707. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004707_01	euxassay_004707_02	euxassay_004707_03	euxassay_004707_04

euxassay_004707_05	euxassay_004707_06	euxassay_004707_07	euxassay_004707_08	euxassay_004707_09

euxassay_004707_10	euxassay_004707_11	euxassay_004707_12	euxassay_004707_13	euxassay_004707_14

euxassay_004707_15	euxassay_004707_16	euxassay_004707_17	euxassay_004707_18	euxassay_004707_19

euxassay_004707_20	euxassay_004707_21	euxassay_004707_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7615_wholemount_strong.wlz

7615_wholemount_moderate.wlz

7615_wholemount_weak.wlz

7615_wholemount_possible.wlz

7615_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7615_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8672
Entity Detected:	Usp19, ubiquitin specific peptidase 19 ( MGI:1918722)
Sequence:	sense strand is shown >T8672 GCGGCTGAAGCAGCGGCGAAGGCGGCGGTGGCGGAGGAGCCGGGACGGGGGAGGGGCGCGCCGGAACCGG AACCGACCCGACGCCGGAACCGGAATCGAGAGCGTGTTGCCAGGGCCCGAAGAGGGCTGGTGGCGGCGGT CTCGCTCGGCTCTTGGTTCTTTGCTGAAGAATTTGGCCACAAAGAGTTGCCAAGATAGCTGGGCCGGGAA GAAAGCGCCGCAGCCCTGACCCAGACGCTGTTGCCGACTCTGGGCCACTCTGGCTGGCTACCAAGCGGCT CAAGATGTCTGCAGGGGCCAGTGCTACAGGGCCCAGGAGGGGGCCGCCAGGACTGGAAGAGGCCACTAGT AAGAAGAAACAGAAGGATCGAGCAAACCTGGAAAGTAAAGATGGAGATGCTAGGAGAGTGTCCCTTCCTC GAAAGGAACCAACCAAAGATGAATTGTTGCTCGATTGGAGGCAGAGTGCAGATGAGGTGATTGTTAAGCT GCGCGTGGGAACAGGTCCCGTACGTCTGGAGGATGTAGATGCTGCGTTCACAGACACGGACTGTGTGGTG AGGCTTCCAGATGGTCGGCAGTGGGGTGGTGTCTTCTTTGCTGAAATACAAAGTTCTTGCACCAAAGTGC AGGCTCGCAAGGGTGGTCTTCTACAGCTAGTACTACCCAAGAAGGTGCCTCTGCTCACGTGGCCCTCTCT CCTGAAATCTCTGGGAACCCAAGAGCTGGTGCCAGGTTTGCAGTGCCAGGAGAACGGGCAAGAGCTGTCT CCCATTGCCCTGGAGCCAGGCTCTGAGCCCCGCAGAGCTAAACAGGAAGCCCGAAACCAGAAGCGGGCCC AGGGCCGTGGTGAGGTAGGCTCGGGGGCTGGCCCTGGGACACAGGCAGGGCCCAGCGCCAAGAGGGCTGT TCACCTCCGCAGAGGGCCAGAAGGGGAAGGGTCCATGGATGGCCCCGGCCCCCAGGGTGATGCCCCGTCT TTCCTGTCTGACTCAGCTACCCAGGTTGAGGCTGAGGAGAAGCTCTGTGCTCCACCAATGAACACTCAAA CAAGTCTCTTGAGCTCAGAGAAGAGTTTAGCCCTTCTGACAGTAGAGAAGACAGTGTCCCCCAGGAATGA CCCAGTCGCCCCGGTTATGGTCCAGGACAGAGACCCTGAGCCTGAGCAAGAAGACCAAGTCAAAGAGGAG ATGGCACTTGGGGCTGATCCTACAGCCTTGGTGGAGGAACCAGAGTCTATGGTGAACCTGGCATTTGTCA AGAACGACTCGTATGAGAAGGGCCCGGATTCGGTGGTGGTGCACGTGTACGTGAAGGAGATCCGCAGGGA CAGCTCCCGAGTGCTCTTCCGAGAGCAGGACTTCACACTGATCTTCCAGACCAGGGACGGAAACTTTCTG AGGCTGCATCCGGGCTGTGGGCCCCACACCATCTTCCGATGGCAGGTGAAGCTCAGAAACTTGATTGAAC CAGAGCAGTGTACGTTCTGTTTCACGGCCTCTCGAATCGATATCTGCCTCCGGAAGCGGCAGAGTCAGCG CTGGGGGGGACTGGAGGCCCCTGCTACACGAGGTGCAGTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGC CCAACCCCTTTGGATTCAACCCCTCCAGGAGGTGGCCCCCACCCCCTGACAGGCCAGGAGGAAGCCAGGG CTGTGGAGAAGGAAAAACCCAAGGCTCGATCAGAGGACTCAGGGCTGGATGGTGTGGTGGCCCGCACCCC CTTGGAGCATGTAGCCCCAAAGCCAGACCCACACTTGGCCTCGCCCAAACCCACGTGTATGGTGCCTCCA ATGCCGCACAGTCCAGTTAGTGGGGATAGTGTGGAGGAGGACGAAGAGGAAGAGAAGAAGGTGTGCCTGC CAGGCTTCACTGGCCTTGTCAACTTAGGGAACACCTGCTTCATGAATAGCGTCATTCAGTCTTTGTCCAA CACTCGGGAACTTCGTGACTTCTTTCACGACCGATCCTTTGAAGCTGAGATTAACTACAATAACCCATTG GGGACTGGTGGGCGCCTCGCCATTGGCTTTGCTGTGCTGCTCCGGGCCCTATGGAAGGGTACTCACCAAG CCTTTCAGCCCTCCAAGCTAAAGGCCATTGTGGCAAGCAAGGCCAGCCAGTTCACAGGCTATGCACAGCA TGATGCTCAAGAGTTCATGGCTTTCTTGTTGGATGGGCTACATGAAGACCTCAATCGAATCCAAAACAAA CCCTACACAGAGACTGTGGACTCGGACGGGCGGCCCGATGAGGTGGTAGCCGAGGAAGCATGGCAGCGGC ACAAGATGAGAAATGATTCATTCATTGTGGACCTGTTTCAGGGCCAGTACAAGTCAAAGCTGGTGTGCCC TGTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTCCTTTATCTGCCGGTACCCTTGCCACAAAAGCAA AAGGTTCTCCCCATATTTTATTTTGCCAGGGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGTGTCA GCAAGGAGAACTCCAGCGCGAGTGAAGTGTTGGACTCCCTCTCTCAGAGTGTCCACGTGAAGCCTGAGAA CCTGCGCCTAGCCGAGGTAATTAAGAACCGTTTCCACCGTGTTTTCTTGCCCTCCCACTCACTGGACGCT GTGTCCCCCACGGACGTGCTCCTCTGCTTTGAGCTGCTCTCCCCAGAACTGGCTAAGGAGCGGGTAGTAG TGCTGGAGGTGCAGCAGCGCCCCCAGGTACCCAGCATCCCTATCTCCAAGTGCGCAGCCTGCCAGCGGAA GCAGCAATCAGAAGAAGAAAAGCTGAAGCGCTGTACCCGTTGCTACCGTGTGGGCTACTGCAACCAGTTC TGCCAGAAAACCCATTGGCCTGACCACAAAGGCCTCTGCCGCCCTGAGAACATTGGCTACCCCTTCCTGG TCAGTGTGCCTGCTTCACGCCTCACTTATGCCCGTCTTGCTCAGCTACTAGAAGGTTATGCCCGGTACTC TGTGAGTGTATTCCAACCGCCCTTCCAGCCTGGCCGCATGGCTTTGGAATCGCAGAGCCCTGGCTGTACC ACGTTGCTTTCAACCAGCTCTCTGGAGGCTGGGGACAGTGAAAGAGAACCCATTCAGCCTTCTGAGCTCC AGCTGGTGACCCCTGTGGCTGAAGGGGATACAGGGGCTCACCGAGTATGGCCGCCTGCTGATAGGGGTCC TGTGCCTAGCACCAGTGGACTCTCTTCTGAGATGCTGGCCAGTGGGCCTATCGAAGGTTGTCCCTTGCTT GCTGGTGAGAGGGTATCTCGGCCTGAAGCTGCTGTGCCTGGGTACCAACACTCAAGTGAATCTGTGAATA CCCACACGCCCCAGTTCTTCATCTATAAAATTGATGCATCAAACCGTGAGCAGCGGCTGGAGGACAAAGG GGAGACACCATTGGAGCTAGGTGATGACTGTAGCCTGGCTCTGGTGTGGCGGAACAATGAACGCCTGCAG GAGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAAGATCCAGGCTCTGCTGGTGAGGCTGCCC GTGCTGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTTACACGGCCTGAAGTGCTGGCACCTGAGGA GGCCTGGTACTGCCCACAGTGCAAACAGCATCGTGAGGCCTCCAAACAGCTGCTGTTGTGGCGCCTACCG AACGTGCTGATTGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTCATTTGGCGAGACAAGATCAATGACT TGGTGGAGTTTCCTGTTCGGAACCTGGACTTGAGCAAGTTCTGTATCGGTCAGAAAGAGGAGCAGCTGCC TAGCTATGACCTGTATGCTGTCATCAACCACTACGGAGGCATGATCGGTGGCCACTATACTGCTTGTGCA CGGCTGCCCAATGATCGCAGTAGCCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACGGTGA CAACAGTAGACGAAAGCCAGGTCGTGACGCGCTATGCCTATGTTCTCTTCTACCGTCGTCGGAACTCTCC TGTGGAGAGACCCCCCAGGGCAAGTCACTCTGAACACCACCCAGACCTAGGCCCTGCAGCTGAGGCTGCT GCCAGCCAGGCTTCCCGGATTTGGCAGGAGCTCGAGGCCGAGGAGGAGATGGTACCTGAGGGGCCTGGGC CTCTGGGTCCTTGGGGGCCCCAAGACTGGGTGGGGCCCCCGCCACGTGGCCCTACCACACCAGACGAGGG TTGCCTCCGATACTTTGTCCTGGGTACCGTGGCGGCTTTGGTGGCCCTTGTGCTCAACGTATTCTATCCT CTGGTATCTCAGAGTCGCTGGAGATGAGCTCACCTGCAGGCACCTGCTGTGAGCTGGCCTACCTGTCTGC TCACCAGGTCATGCCTACCTTTGTGGTGGGAAACAACTCTGGGCTCTGAGCCTCAGTGTATGTATCTGGT GGGAGACTTGTGGGGACTGCAGTCTCTGCAGGGGCAGAGTATCCTAGGGTGTGTGTCCATCCAGTTGTCT GTCGTCCTGTTTCTCTGACCCTTGTCCTAGGGCTTGGCCCTGCTGGAACTATTTCCTGTATTTAAAGTGT TAATAAAATGAGTATTCAGGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400986 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400986 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7613 same embryo
	EMAGE:7618 same embryo
	EMAGE:7617 same embryo
	EMAGE:7614 same embryo
	EMAGE:7616 same embryo
	EurExpress:euxassay_004707 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source