EMAGE mouse gene expression spatial database

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EMAGE:7618

Cerk ceramide kinase ( MGI:2386052)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004689. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004689_01	euxassay_004689_02	euxassay_004689_03	euxassay_004689_04

euxassay_004689_05	euxassay_004689_06	euxassay_004689_07	euxassay_004689_08	euxassay_004689_09

euxassay_004689_10	euxassay_004689_11	euxassay_004689_12	euxassay_004689_13	euxassay_004689_14

euxassay_004689_15	euxassay_004689_16	euxassay_004689_17	euxassay_004689_18	euxassay_004689_19

euxassay_004689_20	euxassay_004689_21	euxassay_004689_22	euxassay_004689_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7618_wholemount_strong.wlz

7618_wholemount_moderate.wlz

7618_wholemount_weak.wlz

7618_wholemount_possible.wlz

7618_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7618_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 weak expression: see section 16 19 20 21 22

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8673
Entity Detected:	Cerk, ceramide kinase ( MGI:2386052)
Sequence:	sense strand is shown >T8673 CCTATCGCCTGCCCAGGACCGCCCGGGCAACATTTCTGCGCTGCTGGGACTCCGCCCGTCCCTTTGCAGC CCTCCCTACCCCGTCGCTCGTGCAGACCCAGCAGGACCCAGGGGCAGCCAGAGCGACAGACTAGTGTCTT TCAGGGCACCCGGCCGCTCCGACCGCCCTCCCCACAGCCAGGTCCCTCAGTCCCCTCCTCTGACCCCTCC CTCCCGTTCCTTCCTAGGCCTTTTCTGCTCCTCATCCTAGGCTCCCTAGACTCCTCTGTTTCTGATTCTT CTCTGGCCCCAAGTCTAGCTTCTCCTGTCCTCACCCTGGTCGCCTCCTCCCGGGTCCCCGCCACCCTCTC TGCGTACCGGGTGACGCAGCGCCGCCTCCCGCTCCCGCCTCCCCGCGTCACCGCCGGGCAGACTGCAGGG GCCGCTAACGGTCCCGCGCCCCCCCGGTGTCCGCGCGCCCCGCGCCCGGCGGACGAGGCCGCGCCGGCGG CGATGGGGGCAATGGGGGCGGCGGAGCCGCTGCACTCCGTGCTGTGGGTGAAACGGCGACGCTGTGCCGT CAGCCTGGAGCCCGCGCGGGCTCTGCTACGCTGGTGGCGGAGCCCGGAGCCCGGGCCCTCCGCGCCGGGT GCCGATGCCCGCTCGGTACTGGTGTCGGAGATCATCGCTGTTGAGGAAAAAGACGACTGCGAGAAACACG CCTCCAGTGGCCGATGGCATAAAATGGAAAATCCGTTCGCATTCACAGTCCACCGTGTGAAGCGAGTACG ACATCACCGCTGGAAGTGGGCACGGGTGACCTTCTGGAGCGCCGACGAGCAGCTGTGTCACCTGTGGCTG CAGACCCTCCGTGGGCTGCTGGAGAGCCTGACTTCAAGACCGAAGCACTTGCTGGTATTCATCAACCCTT TCGGAGGGAAAGGTCAGGGCAAGCGCATCTATGAAAAAACAGTGGCGCCTCTGTTTACCTTGGCTTCCAT CACTACGGAGATCATCATTACAGAGCATGCCAACCAAGCCAAGGAGACTTTATACGAGATCAACACAGAC AGCTATGATGGCATCGTGTGCGTAGGTGGGGACGGCATGTTCAGCGAGGTGCTGCATGGGGTGATTGGGA GGACGCAGCAGAGCGCTGGTATCGACCCCAATCACCCCCGAGCCGTGCTGGTGCCCAGTACCCTCAGGAT CGGCATCATACCCGCAGGGTCCACAGATTGTGTGTGTTACTCAACAGTGGGCACAAACGACGCAGAGACA TCGGCTTTGCACATCATTATTGGGGACTCACTGGCAATAGACGTGTCCTCTGTGCACTACCATAACACGC TGCTGCGGTACTCGGTTTCTCTGCTGGGCTACGGTTTCTACGGGGACTTAATCAAGGACAGTGAAAAGAA ACGGTGGATGGGCCTCGTCCGGTATGATTTCTCAGGGTTGAAGACCTTTCTCTCTCATCAGTACTATGAA GGGACACTGTCCTTCCTCCCAGCACAGCACACGGTGGGATCTCCACGGGACAATAAACCCTGCCGGGCTG GGTGCTTCGTGTGCAGGCAGAGCAAGCAACAGCTGGAAGAAGAAGAGAAGAAAGCCCTGTATGGCCTGGA GAACGCCGAGGAAATGGAAGAGTGGCAAGTGACATGTGGGAAGTTCCTGGCCATCAATGCCACCAACATG TCCTGTGCTTGTCCTCGGAGCCCTGGGGGCCTGTCCCCATTTGCCCATCTGGGAGATGGGTCTTCTGACC TCATCCTTATCCGGAAGTGCTCCAGGTTCAACTTCCTGAGATTCCTCATCCGGCACACGAACCAGGAGGA CCAGTTCGACTTCACTTTCGTTGAAGTTTATCGAGTCAAGAAATTCCACTTCACGTCGAAGCACGTGGAA GACGAGGACAATGACTCGAAGGAACAAGAGAAGCAGAAGTTTGGGAAGATCTGCAAGGACAGACCCTCTT GCACTTGCTCAGCCTCCAGAAGCTCCTGGAACTGCGATGGCGAAGTCATGCACAGCCCGGCCATTGAGGT CAGGGTCCACTGCCAGCTGGTGCGCCTCTTTGCTCGGGGAATCGAGGAAGAGTCATAAGCAAGAACCCCA AAGCCCAGGAGCTGTCGGCCTTGAGCTCGGGGAGTGTGGAAATTACTTAAGAAAAATTCGACAGACCAGT TATGTTGATATATCCATTTGAATTTAGAAATTTATTTTTGATAGGTAAATCTTGGTTTTTAGAAAAGACA GCTTTGTCAACAGTTCTGTATCCATACCTGGTACCCTCGGCCCCGCTTCCGAGTCGCTTTTCACTTCTTC TCAGCAAATGGCACTTGCTGGTTCCGGGCATTGGCTTCCTAGATTTTAGCCATGCCGACCGTGCATATTT CAAGACAGCCCCTTAGTGGGCTACACTGCAATAATTTTTAGGGACATTATAATATGACTACGTCCCAGGA ATCAGGTTACACCATCTGGTCACATGGGTAAAACCCGGTCACCTTGACCCCTTTGGTGGGACTTCCTCTG GAATTGTCCGTGGCCAGGTTTGGGAAGGTAAATAGCTGTCTTGGCCTCCGCTTTACACACACCTTCATTT ACGATCAGGACGTCGCTATGTGGCCCTTGCTGCACACCACAACTGACTCAGCGAGGTCCTCCGCACAGCC ATGCCTGCAGAGCAGGGCCATGTCACAGCGAGAAGACACCCAGGGTGACGTCTCATCCTCAGGATCCCCC ACGTCTGCTGCCATCACAGCCGCATCCCGTGAGACAATTCAGCAAAGCGCCCACTTGGAAGCTTCATTCT GTCAGCCCTGGTAGCCTGCACACCCTGGCCAAGGCAGTCTGAAGCACTGGCTAAGAGGAGGCTCCATTTG GAATCAAAGCACTTCTACAGCTGGGTTTTGAAAGAGGTAGGGGGTGGCGGGGACAGAGAGTCACTGACAC ACACAGAGGGTACTCGGTGAGTTCGATACCACCCTCAACCTGACTGTCACCAGCGAATGGTGGGTGATCT CGGGTCACCCCCAGGTACTCTGGTGCAAGCCTTATGTCTACTCCTGGTTCCACATCCATTCTTCCGCACT GTGGATTCTGTTCTTATTCATAGCTTTCTCCCTATCCATCACGTCTTGGCTATTTGCTCTGTCTCCACAA GGGGGCGCTAAGCTGCTCAGGCCGAAGCCCTGGGCGCATGACCAGTTTTCCTCTGAAGGCATTCCCAACC CAGGGGCTTCTCGGCCACATACTATGGCCTGTGCTGTGTCTCCAGATGGATGCATCTGAAATGTGTATAA AAGGGTTTTATGTGAAATTCAGGCCTACACAGCTTAAACTTGACTTGCAAACGGGGAAGGGCACTGACTG TTACAAGAGCTGGTTTTAGGTTCTGCACTGGGGGAAGTGTGCATCTCGTGCTGGTCCAGTTACTCGCTAC AGAATTCTGCAGGCCACACGGCTTTCCAGGTCCTCTTGCACTGACTTCTGTTTCGAGGCATACACAAAAT CCTCTGTTTAGTATTCAGTCCCATCCAGACAAAAGAGCAGCTGTAAAGGCGGCCATGTTCAGGGACCACT TTAAAGGCAGTTGTGCGGCGGTGCTATTTAATGATTGTCGAGTAGAAAGCTGGCTAGAAAGAGTAAATGA ACTAGCAGTTTCTATCCAGATCTGTCCCTGGATGGGCAGAGCCCAAAGGTGCCAGCCTAGCAGCTCTGTG AGTAGGTAGGCAGCCCTGGAGCAGCGTGAGGGCCGATGCTCAGTTGTCCACCTCAATGTGGCCCCTCGCC CCCGGCATTCCAGAGGGGGCTCAGGGCCCGAGGCAGCTGTGGGTGCCCCTGGATCTCAGGAATTGCACTG TCCTCCAAGGACTCAGGTCTTCACTGACCTTGCTACATGATACCCACGCCCTGGTCCACCCCTGCCGCTG CCAGGGCTGGAGGAGAGATGCAGTCAGAGGATGCTTTGCCGGTGCTTAGCAGTGCAAGCCACGCTCGGAA TCTCCAACCCCGGCGCAGTCATGGAGCATCATGAGTGTCAGACACTTAGGGCCCTTGACACGGCTGCGCA GGATTGGGGGGGGGGGCGTGCTGACATTCACAAGCAGTAGACAGTCTTCATGATGCTTTTTAGATGCAAT ACCCCAAACTTCCCGGGTCTTAGGTATTTTAGGAGGAAAAAACAGGGGTAAAGGTTAGACAGTGGCAACT TAGGATCCTGTTAGGGTCTGGCCCATGTTTTAACTCCCACTGGTGCATACCAATTAGGCAACAGTCATAA GCTGTAACTTATTTTTGTTTATGGAAACTAAGTAGTGGGAACGGGGACCGGGGAGCGGCTGGTTTCCCAG AGGATGCTTCAGCCAATGCAATCTCCTGGAGCTGGGAGCACTGACTGGGAGCACTGACACAAGAATGGCC CAATTATGCCTCCTGGTCTTGCTGGCTTCAAGATTAGAAAGTGGCATATTTGATCTGGTATATTTGAGAA GACAAAAAAAATAATAATAAAGTTACTTTGGGCAGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400872 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400872 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7613 same embryo
	EMAGE:7617 same embryo
	EMAGE:7614 same embryo
	EMAGE:7616 same embryo
	EMAGE:7615 same embryo
	EurExpress:euxassay_004689 same experiment
	MGI:4823820 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source