EMAGE mouse gene expression spatial database

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EMAGE:7632

Olig3 oligodendrocyte transcription factor 3 ( MGI:2149955)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004726. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004726_01	euxassay_004726_02	euxassay_004726_03	euxassay_004726_04

euxassay_004726_05	euxassay_004726_06	euxassay_004726_07	euxassay_004726_08	euxassay_004726_09

euxassay_004726_10	euxassay_004726_11	euxassay_004726_12	euxassay_004726_13	euxassay_004726_14

euxassay_004726_15	euxassay_004726_16	euxassay_004726_17	euxassay_004726_18	euxassay_004726_19

euxassay_004726_20	euxassay_004726_21	euxassay_004726_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7632_wholemount_strong.wlz

7632_wholemount_moderate.wlz

7632_wholemount_weak.wlz

7632_wholemount_possible.wlz

7632_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7632_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus

moderate

regional

moderate expression: see section 11 12 13

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 19 20

4th ventricle

moderate

regional

moderate expression: see section 03 04 05 06 07 08 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8717
Entity Detected:	Olig3, oligodendrocyte transcription factor 3 ( MGI:2149955)
Sequence:	sense strand is shown >T8717 TGTTACCATTCTGATGTTGGAGCGGCCGCTGAGAGAGCCTTTTTTTTTTTTTTTTTTTTTTTAATTCAAG ATTCTCTTTAATTTCCTGCCTAAAGGCTCCTCAACCATAAGGCCGCATCTCTTGTAATAAAATGAATTCT GATTCGAGCTCTGTCTCCAGCAGAGCGTCATCTCCGGACATGGATGAGATGTACCTAAGAGACCACCACC ACCGCCACCACCACCACCATCAGGAGAGTCGTCTGAACTCGGTCTCCTCCACACAGGGCGATATGGTCCA GAAGATGCCAGGGGAAAGCCTCTCGAGGGCAGGCGCCAAGGCTGCAGGAGAAAGCAGCAAGTACAAAATC AAGAAGCAGCTGTCGGAGCAGGATCTTCAGCAGTTGCGGCTGAAGATCAACGGACGCGAACGCAAGAGGA TGCACGACCTGAACCTCGCCATGGATGGTCTGCGCGAGGTCATGCCCTACGCTCACGGGCCCTCGGTGCG CAAGCTCTCCAAGATCGCCACTCTGCTTCTGGCCAGAAACTACATCCTCATGCTCACCAGCTCCCTGGAG GAGATGAAGAGGTTGGTTGGAGAGATCTACGGGGGACACCACTCGGCCTTCCACTGTGGGACTGTGGGGC ATTCGGCCGGCCACCCGGCACACGCAGCCAACGCTGTGCACCCGGTGCACCCTATCCTGGGCGGCGCGCT CTCCTCTGGCAATGCCTCGTCCCCTCTGTCCGCGACCTCCCTGCCGACCATTGGCACCATCCGTCCTCCC CACTCACTGCTCAAGGCGCCCTCCACGCCGCCCGCGCTACAATTGGGCAGCGGCTTCCAGCACTGGGCGG GGCTGCCCTGTCCCTGCACCATCTGCCAGATGCCACCACCGCCGCACCTGTCCGCTCTCTCCACCGCCAA CATGGCCCGGCTTTCGGCTGAATCCAAGGACTTGCTCAAGTGAGCCACGGGCTGGCCCGGCCGGGAGGAG GAAGGGAGAGCTGAGGCAGGGGTCGCAGTGCCGGGCCAGGAGGGAAAGGGAGACTGCTCGGCCTGCGGGA GGGTGGGCGCGCGGCCCCAGGCCTCGCCAGCGCCCTACAGATCCAGATTCCAGTTGACCCACCCCAGCGG GGCGTGCCAAAACCAAACTGGAATACCTAGGTGTTAGCGAAGGGGACTGGAACCCGAAGTCCTGTTTTGC ATGCATGCTTCTTTCCCTCATGCCACGCCAGTATTTCTCACTGTGGTATGCTCTTGTCAAAACTGCATTT GTTGTTTAACGATGGGATCAGTGGAGTTTAAAACTTGCAAAGGAACTTTGGAGTTGGCTCTTTCTGGCTG TGTTCTTATCTTGGAGGAAAGATGGCTATCCCTTTTGTGCAAAGCAAATGTAAATATTTCATCGAGCTAG ATACACTTATGATTGTCTCTGCCCTTATAGATTTTTCAGAGACAATGGAGAGAGAGAGAGAGAGAGAGAG AGAGAGAGAGAGAGAGAGCTTGTTTCAGAATCCAAGTTTTGATTCTCAGCCCAGGGCATGAACCTCTTAT GTGGGTTTTCCTTTCCACGCAAACATAGGTCAAGAAAATGCATGCACGCTTCTAAATGAATCTTGTCTAA ACATGTTGTGCGGGCTATAGCGCCATTTTCCTTGTAACAAGGATTTATTAGTGAGCTCTCCAAAGTACTC CATATATCTGTCTGTGGTTGTATAAAATGAAAAGGGGGCCAGAGCACAGAGATTCACACTCAATGAACAT GTGATTCTTCCCCCAAGCCCATGTCTGTCTCTGTCCCTCAAATCTCATTCCAACGTTAGGGCTGTTCCAA GGGGCCCACTACAAAACCGGGCCTCCTCTAGAAGTTCATCCTACCCCCTTGCCAAATTATACTGCATGCG GGGTTGGGTCGGTTACCCCTCAGCTTTGAAAACACACTGGGTTTTTGGGGAGGGGGCTGCCACTTTTATT TTCCTGAACATTTGCTTGAATATTTTGTTAGGGCCTCCAAGACAATACCTGTCTTTATTTTTTATATATT CTTGATAAGTATGATATATTTATTAATACCAGTGTTTCTGGATGAGATTTCAAATAAAAGAGAACCTTTA AATCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6403310 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6403310 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7634 same embryo
	EMAGE:7631 same embryo
	EMAGE:7633 same embryo
	EMAGE:7636 same embryo
	EMAGE:7635 same embryo
	EurExpress:euxassay_004726 same experiment
	MGI:4826969 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source