EMAGE mouse gene expression spatial database

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EMAGE:7634

Esm1 endothelial cell-specific molecule 1 ( MGI:1918940)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004719. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004719_01	euxassay_004719_02	euxassay_004719_03	euxassay_004719_04

euxassay_004719_05	euxassay_004719_06	euxassay_004719_07	euxassay_004719_08	euxassay_004719_09

euxassay_004719_10	euxassay_004719_11	euxassay_004719_12	euxassay_004719_13	euxassay_004719_14

euxassay_004719_15	euxassay_004719_16	euxassay_004719_17	euxassay_004719_18	euxassay_004719_19

euxassay_004719_20	euxassay_004719_21	euxassay_004719_22	euxassay_004719_23	euxassay_004719_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7634_wholemount_strong.wlz

7634_wholemount_moderate.wlz

7634_wholemount_weak.wlz

7634_wholemount_possible.wlz

7634_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7634_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

other

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pineal primordium

strong

regional

strong expression: see section 14

neural retina

strong

spotted

strong expression: see section 02 03 04 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8714
Entity Detected:	Esm1, endothelial cell-specific molecule 1 ( MGI:1918940)
Sequence:	sense strand is shown >T8714 GAGGCTGGCAAGAGCACAGCGCTGCTGCTGGGTTGAGGAAATTGATGACGGGGAAGCATGCGGGCAACAC AGTGTATAAAACTCATAACCGTGTAGGCTGAAGCTCAGCTACTACTTTGGACGGCTGCTTCCCTCCAGCA AAGACCACGACGGCAGGGAGCTGAGCAGGAGCCAGCTGCGAGACATGAAGAGCCTCTTGCTGCTGACCAC ACTCCTGGTACCTCTGCACCTGGGCATGGCCTGGAGCGCCAAATATGCGGTGGATTGCCCGGAACATTGT GACAAAACTGAGTGCAGAAGCAGCCTGCGTTGCAAGAGGACAGTGCTGGATGACTGTGGCTGCTGCCAGG TGTGCGCCGCGGGACCTGGAGAAACCTGCTACCGTACAGTCTCAGGCATGGACGGGGTCAAGTGTGGTCC GGGGCTGAAGTGTCACTTTTACAGCGAGGAGGATGATTTTGGTGACGAGTTTGGTATCTGCAAAGACTGT CCCTATGGCACCTTCGGGATGGAATGCAAAGAGACTTGCAATTGCCAGTCGGGCATATGTGACAGGGTGA CCGGAAGATGTCTGGACTTTCCCTTCTTCCAGTATGCAGCAGCCAAATCTCCCAGCAGGACCTCTGCCTC CCACACAGAGCGTGACTCAGCATCCGGAGATGGCAACGCTGTGAGAGAAGAGATTGGAGAAGGGAACGCT GCTCGGCCCTCCGTGATGAAATGGTTAAATCCACGCTGATCCTGGCTGAGATTTGCCAAGAGAAGGCTCC ATTTTAGTGACCAACATTCAGCCAACATTTTAAGAACGGTCTAGAACATAGGCTGTGGACATGTAATTTA TGGAGATCAAGTGTGATTCAGAAAAAGAATGTAGGCTACATACAACCCATAAGACACATATGACTGAACC CTTTTAGGTATTTGCTAAATATTTAAATTCATATAGATCTGCTAAGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGGTATCACCAAGGAACTTGAGATGGCCTTGCTGTCGATGAGTAATCCAATCAAAGCT GAAGGACCGACCGGTGGTTTCATTGACCTGGGTGCACATTCTTATTGTGATACAGGTTGAAGAAGAGTTG GGAGCAGGAGACAGTGGGATCGTTGTGAAAGTAGGGGTTATAATATTTGTGTCCTCCTTGTAGTAGTTTA AAATTTTTTTATTTTGTCTTACTTGGAGGCGATAAAACAAGACCAGAAAAGCCTTGAAGGAAGTGAGATA TTGGAAGCTGAACAACTGCTTTGAATGGAAATGCTGTTATTACAGCTCCTTGTATCTGATGAGAATGGGG GCGGGCAGAGGAGGTGGACAGAGGAAAGAGTATATATTATATTCCCATCACTTCTCATCTCTGGTTTAAA CACTGTGGGCCTTGAGACAGATGTAGAAAGTAGTTTGCCCTCCTGAGGGACAGAACAGGGTGAAGGTGAA TATTTATGTATTTTTATAAACAAATCTTGTAGTTTAAATATTTCTCCAGCACCCATATGTCTCCCCATCT TAGAAAAGTTACCCTAATGTTGCTTGTTTAAAATGGGTTCTCAAATACTCATATATGGAAAATGTGTTAT TAACAATTGCACTAATAGTAATAAAATGTAGTTACTCCAGGTGTATAAAATTTTGAAAATTTTTTTAAAT GCTATATCTTGAGTGAATTTTAAATTTAAATCAAAAATTAACTTTTTAGACAGTTTGAGAAAGGAAAGTT AACTTTCATTAGGAAATGTGTCAGCTTTAAGGTATAGTTTATTTTCATTTTTTAAAATTCTTTCTAACTT GATTGATGAATGGACCTGAGTTTTTACCCGGAGGACCTTAGCACAAGAAGAACTGTTGTCCTGCCAATGC TGGGAGATATTTGCAGAGTATTTTATTTGGAGTGAAGAACTTATTTAAGAAGTATTTGCTTATTTCCTTA TATTTTATTCTCAAACCTTGCCAACAGAGTTGTGAATGTACTCGTGGGAAGATCCGTGAATGTAAGCGCA ACCGGCTGTTAGGTTTTGTCTTAAAAGAACGGTGTATTATTGTTCAATAAAAATGGCACAGCTGTTCCAT GTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6403364 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6403364 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7631 same embryo
	EMAGE:7632 same embryo
	EMAGE:7633 same embryo
	EMAGE:7636 same embryo
	EMAGE:7635 same embryo
	EurExpress:euxassay_004719 same experiment
	MGI:4824623 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source