EMAGE mouse gene expression spatial database

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EMAGE:7651

Zfp866 zinc finger protein 866 ( MGI:3584369)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005302. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005302_01	euxassay_005302_02	euxassay_005302_03	euxassay_005302_04

euxassay_005302_05	euxassay_005302_06	euxassay_005302_07	euxassay_005302_08	euxassay_005302_09

euxassay_005302_10	euxassay_005302_11	euxassay_005302_12	euxassay_005302_13	euxassay_005302_14

euxassay_005302_15	euxassay_005302_16	euxassay_005302_17	euxassay_005302_18	euxassay_005302_19

euxassay_005302_20	euxassay_005302_21	euxassay_005302_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7651_wholemount_strong.wlz

7651_wholemount_moderate.wlz

7651_wholemount_weak.wlz

7651_wholemount_possible.wlz

7651_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7651_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8947
Entity Detected:	Zfp866, zinc finger protein 866 ( MGI:3584369)
Sequence:	sense strand is shown >T8947 GGCTCTGCGGCACAGGGAGGTGCGGTGTGTTGTGCTGTGAGCGCTGCTGCCGGGACCAGTGAGGAGAGCG CGGGCGAGTTCGGAAGCCGCGCCATGGATTTGGTGAGCTTTGAGGACGTGGCTGTGCACTTCACCCAGGA CGAGTGGGATTTGCTGGACCCTTTCCAGAAGAGTCTGTACAGAGATGTGATGCTGGAGACCTGCAGGAAC CTCGCTGCGATAGGGTATATATGGGAAGAACAGAATGTTGAAGATGACTGCAAAAATTTTGGAAGAATTC AAAGGCATGTCATATCTGATTCTGAATACTTACTGGATGAGCAGGAGGGATATGGAAACAAGCCATACGA TTCTAGTTCTCTCACAAGTATTCAAGGACACATGGGAGCGCACACTGTGAATGGACCTTGTGAATGTGAG GTATGTTTAAAATCCTTTGGTTTTCCAAGTGCATTTGGAATGTGTCACCAGCCCCACAGTGGACAAAACG TTTGTGACTACAAGGAATTCGGAAAGGCTTCTGGTTACCACAGTTCACTTTACACACATGGAGGGACTGA CACTGTAAGAAAATGTTACTCGTGTAACCAGTGTGGGAAAGCTCTGAGTTCTTCCAGTTCCCTTCAAAGG CATGAGAGAATTCACACAGGAGAGAGACCCTATAAATGTCAGCAGTGTGGGAAAGCTCTGAGTTCTTCCA CTTCACTTCAAAGGCATGAGAGAATTCACACAGGAGAGAGACCCTATAAATGTCAGCAGTGTGGGAAAGC CTTTAGATGTCACAGTGCCCTTCAGTTACACGAAAGAATTCATACTGGTGAAAAACCCTATGAATGTCAG CAGTGTGGGAAAGCCTTCACATGTCACAGTTACCTTCGATTACATGAGAGGACTCATACCGGAGAGAAAC CTTATGAATGTAAGCAGTGTGGGAAAGCCTTCAGATGTCAAACGTCCTATCATAGACATAAAATTATTCA TGGTGGAGAAACATTCTATGAATGTAAGCAATGTAGTAGGCTCTTCATTTATCCCTCTTTACTTCAAATG CATGAACGAACTCACACGAGTGAAAGACCATATAAATGTAAGGAGTGTGGAAAGTCCTTCCTTTATCCCT CTTTACTTCAAATGCATGAACGAACTCACACAGGTGAAAAGCCCTATGAATGTAAGCAATGTGGTACAGC CTTTAGACATCACTCTTCCCTTCGGTTGCACGAAAGAACTCACACAGGAGAGAAGCCCTATGAATGCCAG CAGTGTGGGAAAGCCTTCACGCGTCACACTTCCCTTCGGTTACATGAGATAACTCACACTGGAGAGAAAC CTTACAAATGTAAAGAATGTGGGAAAGCCTTCAGACATCATTCTTCTCTTCGATTGCATGGAAGAAATCA TAGTGGAGAGAAACCCTATGAATGTAAGCAATGTGATAAAGCCTTTATACGGTACAGTTACCTTCGATTA CACGAGCGCACACACACGGGAGAAAAACCTTACGAATGCAAACAATGTGGGAAAGCCTTCAGCCGCCACA GTTCCTTTCAGAGACATAAATCTATTCATGCTGTGGAAAACCCCTATGAGTGTCAGCAGTATCTAATTCC TTTACCCTTATTTCCTCCAAATACATCAGACAGCTCATACTGGTGAGAAACACTATGAATGTGAACAGTG CTACAGTGTATTCTTATCCAGTGAAAGCATGTGAGAACAGAAGCTGTCACAAGATAAGCCTTCTCCACAT AAATGATACAGTTACAAAGGAATGCATGCCAGGCACAGTGTCTACAAATGTAAGCAGTGTGTGGCGGGCC CTTTATTGGTCTTTACTTGCCTTTCAAGGAAGGAAGTTAATTCATGTTGGGGAAATGCCTTATGAATGGA AGCAATATGGTGAGTCTTAGCCCTGTTTGCTTCAAACACATGAAACACTTAAGGTGCAAAATGCTGTGAC TGTCAACAGCTGCAGCCTTCCAGAGTTGCAGGTCCCTTTGGTGTCGTGAAGGACATCTTACTGGAGGAAA AAACCCTTGCTGTTTAGATGTCATTGCTCCCATAGATTCCATGAACAACTTCTGTTGGAAACAATGTCTG TGAGTGGCAACAAAGTTGTAACACCTTCAAGCTCAGCTCCTTTCAAAGATGTACAAATACTAATGGTGGG ACAAAGCCTATCCAAAGAAAAATGGTGTTGGTTTTGGTTGTCAGTAGATGGACAGACATTTCTGTCAATA GTGCAGCTGCCTGTAAGTTGCCCGTGCTCAATAAGCCCAATTAAAGTCAGTGGCTCCCCAGGCTGCAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6419770 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419770 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7654 same embryo
	EMAGE:7652 same embryo
	EMAGE:7655 same embryo
	EMAGE:7650 same embryo
	EMAGE:7653 same embryo
	EurExpress:euxassay_005302 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source