EMAGE mouse gene expression spatial database

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EMAGE:7653

Tulp3 tubby-like protein 3 ( MGI:1329045)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005317. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005317_01	euxassay_005317_02	euxassay_005317_03	euxassay_005317_04

euxassay_005317_05	euxassay_005317_06	euxassay_005317_07	euxassay_005317_08	euxassay_005317_09

euxassay_005317_10	euxassay_005317_11	euxassay_005317_12	euxassay_005317_13	euxassay_005317_14

euxassay_005317_15	euxassay_005317_16	euxassay_005317_17	euxassay_005317_18	euxassay_005317_19

euxassay_005317_20	euxassay_005317_21	euxassay_005317_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7653_wholemount_strong.wlz

7653_wholemount_moderate.wlz

7653_wholemount_weak.wlz

7653_wholemount_possible.wlz

7653_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7653_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8944
Entity Detected:	Tulp3, tubby-like protein 3 ( MGI:1329045)
Sequence:	sense strand is shown >T8944 GGTATGGAGGCGGCGCGCTGCGCACCCGGACCCCGCGGGGACAGTGCCTTTGACGATGAGACCCTGAGGC TTCGGCAGCTGAAGCTGGACAATCAGAGGGCACTGCTGGAGAAGAAGCAGAGAAAGAAGCGCCTTGAGCC ACTCATGGTACAGCCAAACCCAGAAGCCAGGCTGCGAAGGTTAAAGCCACGAGGCAGTGAGGAGCACACG CCTCTGGTGGACCCTCAGATGCCTCGCAGCGACGTCATCCTACACGGCATCGACGGTCCAGCTGCTTTCC TTAAGCCAGAGGCTCAGGATTTGGAAAGCAAGCCTCAGGTTCTCTCTGTGGGCTCCCCTGCCCCAGAAGA AGGCACGGAAGGGAGTGCAGATGGGGAAAGCCCCGAGGAGACAGCCCCCAAGCCAGACCTTCAGGAGATT CTCCAAAAACACGGCATCTTGAGTAGTGTGAACTATGACGAGGAGCCTGACAAGGAGGAAGATGAGGGGG GAAACCTCAGCTCCCCGTCGGCTCGCTCAGAGGAGAGTGCTGCAGCCAGCCAGAAAGCAGCCTCGGAGAC AGGAGCTTCCGGTGTGACAGCCCAGCAGGGTGACGCCCAGCTTGGAGAAGTGGAGAATTTAGAGGACTTT GCGTATAGCCCTGCCCCTCGGGGTGTCACGGTGAAGTGTAAAGTAACCAGGGATAAGAAAGGCATGGACC GTGGCCTCTTCCCCACCTACTATATGCACCTGGAACGGGAGGAGAACCGCAAGATATTTCTTCTAGCCGG TAGAAAGCGGAAAAAGAGCAAAACGTCCAACTACCTGGTCTCCACAGACCCGACAGATTTGTCTCGTGAA GGGGAAAGTTACATTGGCAAACTCAGATCCAATCTCATGGGGACCAAGTTCACAGTGTATGACCATGGTG TCAACCCAGTCAAGGCCCAGGGTCTGGTGGAGAAGGCCCACACCCGCCAGGAGCTGGCTGCTATCTGTTA TGAAACAAATGTCCTCGGTTTCAAAGGTCCCAGGAAAATGTCTGTGATCATCCCGGGGATGAACATGAAC CATGAGCGCATCCCTTTCAGGCCACGAAATGAACACGAGAGCTTGCTTTCAAAATGGCAAAACAAATCCA TGGAGAACCTGATCGAGCTGCACAACAAGGCCCCGGTGTGGAATGACGACACCCAGTCCTACGTGCTGAA CTTTCACGGCAGAGTCACCCAGGCATCCGTGAAAAACTTCCAGATCGTCCACGGGAATGACCCTGACTAC ATCGTCATGCAGTTTGGACGCGTGGCTGATGACGTGTTCACACTGGACTATAACTACCCACTGTGCGCAC TTCAAGCCTTCGCCATCGGGCTGTCCAGCTTTGACAGCAAGCTGGCGTGTGAATGAGAAGCAGCAAGCAG GGGATTTCTTCACCACAGAGCCTTCAAGAGCAGAAAGCTGCTGCCTCTGCCCCTGCCCCTGCCCCTGCCC CTGCCCCTGCCCCTGCCCCTGCCCCTGCCCCTGCCCCTGCCCCTGCCCCTGCCCCTGCCCTAGCACAAGA AGAGCAACATTCTGCCCCTCTGGTGAATGATCCAGTGTGTGTGTGTGTGTGAATGTGCATGTGTGTGTTT GTTTATACATGCATACACATTCACACATGGATGCATGCTTCCACGTACTCGTTCATGCCTTCTCTGAACT TCAGAGACCTGGTCATAGATTACAACTTCTCATGTGTGAGAGATGGCTTAAAGCACAGGGAAGTAGACAC CCAGGCACACTGGTGTCTCCTGATCGCACAACAGACTCTGTGTGGTGAGTGTAGCTTTCATGTGCTCGGG CACTTCCAACAGAATCAGGTTCTAATGATTTAATACTTGGGCCTCCTGTTTTGAAGGCCCTTTCAAAAGG GGGCTTTTCCCTTGGATGCCTTTAGCCCAGGAGCCTGCAGCCTTCCTCAGCCAATGGTCTGATAGTGTCT CTTGCACTGACCCCAGTCAGGTCCTTTAGCACAAAACAGCCATAACTAGTTGGTGCACAAAGAGTGTAGC TGTGTCCCAATAAAACTTTATTTACAAAGTGGGCATTGGCCTAGTTTGGCCTGTGACTCTGGTTCGCAGG CCTGCCTTGGCTGTGTCCCTGTCCTCCAAGGCTGGAGCATCTTCCAGGCTAGATGTGGAGAGGCGGGGGG TGGTTTACTTTTCAGTAGCAGCAGACTGACATTTTATTTTTATTTTTACTTTATTTTTTTTTCCCATGGA TCTCTGTGTAGCCCTGGCTATCCTGGAACTTATTCTGTAGACCAGGCTGGCCTCAGAGATCCACCTGCCT CTGCCCCCCAAGTGCTGGAATTAAAGGCTTACACTACTGTGCCTGGCCATAGACAGACTCTTAAGAGGAA TTCTTGCCGAATCTACTCTTGGTAGCTTTTGCCTCATGGAGATTATGGCTGCAGAGTGTCATATATCATC TTGGGACATGTGACACTAGTTTGTTCGTGGGTCTGAAAGGCTCTGCTGTCCCCAGCCTGCCTCTTGCTTA TTCTCAGAGTAGGTTGGTGGGCAGCCATCTCAGAAGCATGACTGTGGCCAGATCTTCGGAATCTTTGCTC TCTGGAATCTTAATCTAGTGTCTCCTTAGAGGCAGCAAGAGTCTTGGTGAGGAGGTCACCTCGCCGACAC TCCACAGGCTTTGCCTGTCAGCTTTGTCCTCCAGCCTCGAAACCTGGCCCACACCAGTGGAGACACTGCC CTGCATGCTGAGTCTTTGAGATCTTGAACATCTCGAACATCTCCCAGCGAATCATGACAAATTCCTCAAG GTCCCCAGCCTACAGTGTACCTGAACTTAAGAATTCAAAAACTTTGGGTCTACATTCAGTGGTCCCCCAC TCTCAAACTGGCAACTGCTCCGTGGTTTTTCCTGTATGGAGGAGGTCCCTGCATCCCTGCATCCTGTTGC TCCATGCTCACAGCTGTGTGACGACTACAGCGTGAGATCTGGGAGGTGAGACCTGTGCTAAGGCCAGTGT CTTGCCTGTCCCTTCAGATGTCACTGTCTCTGCCTCTGGCAGGCGTCCCATCAAGTGCCTTGTTCATAAA TGTGCTCTGAAACCTGACACACCTCTGCTTTCCCAGACGAGCCACCTTTGTTGAGTGAAAACTGCTCTCT GTCTCCACTTGAAGGGGGTGGGGCTATGACTTTCTGCCCAGCCTAGAGAGCTGTATACTCCCATTTCATG GACTCTGCTTGAGTGTGCAGGTTTTACATGAATTCTATTTATACCTTTTGTATGCCACATAAATAAAGAT AATTTATATAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAT
Notes:	The probe template was PCR amplified from IMAGE:6419643 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419643 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7654 same embryo
	EMAGE:7652 same embryo
	EMAGE:7651 same embryo
	EMAGE:7655 same embryo
	EMAGE:7650 same embryo
	EurExpress:euxassay_005317 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source