EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7655

D4Wsu53e DNA segment, Chr 4, Wayne State University 53, expressed ( MGI:106498)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005303. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005303_01	euxassay_005303_02	euxassay_005303_03	euxassay_005303_04

euxassay_005303_05	euxassay_005303_06	euxassay_005303_07	euxassay_005303_08	euxassay_005303_09

euxassay_005303_10	euxassay_005303_11	euxassay_005303_12	euxassay_005303_13	euxassay_005303_14

euxassay_005303_15	euxassay_005303_16	euxassay_005303_17	euxassay_005303_18	euxassay_005303_19

euxassay_005303_20	euxassay_005303_21	euxassay_005303_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7655_wholemount_strong.wlz

7655_wholemount_moderate.wlz

7655_wholemount_weak.wlz

7655_wholemount_possible.wlz

7655_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7655_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8946
Entity Detected:	D4Wsu53e, DNA segment, Chr 4, Wayne State University 53, expressed ( MGI:106498)
Sequence:	sense strand is shown >T8946 TTTGGCAGTGGAGGTTCCTGTGCTCTCCGGTCGCTGGAATCGGGAACCTCGCCCGCTTCGGTTCCTGGTC GCAGAGAAAGGTGCCCGCTTGGCCTCTCTCGCCCTCGCGAGCTCTTCATCCGTTTAAGTTCCTAGACGCC GTTGCGTTTGCGGACCGATCTCCGTTTTCAGAAGGGAGCCATGTCCTCGGCCGCCATGTCTAAATACGTG AACGACATGTGGCCCGGCTCTCCGCAGGAGAAAGCCTCGCCGTCCACCTCGGGCTCGGGCCGCTCCAGCC GCCTGTCGTCGCGCTCCCGCAGCCGCTCTTCGTCCCGCAGCTCCCGACGCGACTCCCGCAGCTCTAGCCG CTCGTCCTCGCGCAGCCACAGCCGGCCGCGGAGGAGCAGACGGTCCCGATCCCGGTCCCGCAGGCGCCAC CAGCGCAAGTACAGGCGCTACTCACGCTCGTACTCACGCAGCCGGTCGCGCTCCCGCAGCCACCGCTATC ACCGCGACAGCCGTTACGAGCGGCCGCGGAGGTACTACAAGTCGCCGTCGCCCTACCGCTCCCGGAGCAG GTCGCGCTCCCGCGGGAGGTCGCAGCACCGGTGGTCGTACTACGCGATCACTAGAGGGCGGCGCTACTAC GGCTTCGGCCGGACGGTGTACCCCGAGGATCGCCCCAGGTGGAGGGAAAGATCCAGAACCAGGTCGCGCA GCAGGAGTAGAACCCCATTTCGCCTGAGTGAGAAAGATCGAATGGAGCTACTAGAAATAGCAAAAGCCAA CGCAGCAAAAGCTCTGGGAACAGCCAACTTCGACTTGCCAGCAAGTCTCCGAGCCAAGGAGGCAAGCCAG GGGACAGCTGTTTCCAGCAGTGGGCCAAAGGTGGAGGTAAGTGCCTTCAGGAAGCTGCTGCCAGCATTTA TTACCCTTTGCCAGTGAACACAACCCTCCTGGGGTTCTTTATATCATTTGTCCAGAACAAAACCTTAATC TGACAAGTATCAGCCTGTCTCACAGGAAACTGGGAGACGATGGACTACTTTCGTGTCGACACTTGGACAC TTGGTTTGCTCTTTACACTACAGTAAAAACAGTACATTGTTGGTAATACTTTCTCTCCCTCTAGTCTCTA GAGACTGGAGTGTTGTCAGTAGTTTTCATGTACACCATTATGTTCCCAAGTCTGTGCTCAGCATACAGTA TAGTTTATCTGCTTTATTTCTGTCTTATAGAAATCATGAATGTGGTCTCCAGACAGTGATGAAGAAAATC TGTTGGTAATTGATGATGGGTTCAAGTGTCAGAGTTTATGTTTACAGACTAAAGCTTAGCCATTGTTGAA GGGAGTTGGTGTGCATGCTAGAGTTTTGGGCTAGGGACTGTTTGTGTAAGGCAGTGACAGCTCAGCAGCC TAGAGGCACTGTCATGGTGGGCATCTGTGATCTAGACGTCCTTCACATGATATATGCAATATATTCCAGA TGTTTTGAGAGATTACAGAAGAAGAGGCCTGCTTCCCTTGCAGATAAGTTTATTACGATTCTCCAGAACT ACAGAGGGTGTGCATTGGCACACTGCAGTATATAAGTTAAACCTGGATCTTAAGGTTAATAATTTGGGCT GGGATGTAACTCAGTTGTAGAGAACTTTGTCAGGGTGCATAGTACCTGGGGTTAGCTCCCAGCACTGTGT AGACTGGGTTTAGTACCCCGCTCCTATGATCCCAGCACTTGGGAGGCTGTCCTTGGACAGCTAGGGGATA GATACAAGAGACTTGGTCTCAGAAACTGCTTTATAGAAACATAAGGTAAAAAATAACTATAATGAAGGTG GTTTGTCTGTCCATTGCTGAGACCTCAGGGGCTGCCTGGTCTTTGCTCAGGCAAAGAGGGGCTCCTTGAT GAGTGGATTGCAAAAGCCTCACTTCTTTAGATGAAGCATTTTTTATTGTACTTACTCAAAATGAGGTATA TCTACAACAGGAGTCGGGTATTCGTGGGGAACTGCGCCTCCCAAGTCATTCTGGCTAATTGGAAAACAGG CTTTTTTTGTTGATTAGCTCTTTAACTGTGACAATAGATGGAATTTGGTTTGTGTATCTACAGCTACATT TGTGTACGTTGGGCTTTTTTTTTTTAAATACAATATTTTAAAAGTTAAGGCTATGTTTAAAATTCTCTTC CTCTTTAGCATTCAGAAAAGCAGACTGAAGATGCAACTAAAAATACCAGTGAAAAGTCTTCTACACAAAG AAACATAGCCTTCAGCTCCAATAATTCCGTAGCAAAGCCACTGCAGAAAACAACTAAAGCTGCTGTTGAA GAGAAGTCTTCAGGATCACCAAAAATAGATAAGAAAAAAAGTCCTTACGGACTGTGGATACCTGTCTAAA GGAAAACTAAGGGCTGGGGTTGCCAAAACTGCACTTTACTGAGGGGTTTGTGTCCAGCATTAGATCGTCT GCCTGAGTGATCACAGCAGTAAATGTGATTAAAATCCATTACAGAAAGAGACTTAATGGGGACGTTCATG GTTGAGGTTTCTCTTGAAGATAGGAGGACACAGATGACCCAGAGAATTGCATACAGGTGGGAATCTTGTA TATATCTGTAAGTATTGTATAGAATTGCTTATATACAAAGGTGAATTTTTTAAAGACACAGTTGTACACT GAATTTTGTATATTTGTTAATAAACTTTTTATTATTTGTCTTGATAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6419597 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419597 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7654 same embryo
	EMAGE:7652 same embryo
	EMAGE:7651 same embryo
	EMAGE:7650 same embryo
	EMAGE:7653 same embryo
	EurExpress:euxassay_005303 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source