EMAGE mouse gene expression spatial database

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EMAGE:7666

Reep1 receptor accessory protein 1 ( MGI:1098827)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005277. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005277_01	euxassay_005277_02	euxassay_005277_03	euxassay_005277_04

euxassay_005277_05	euxassay_005277_06	euxassay_005277_07	euxassay_005277_08	euxassay_005277_09

euxassay_005277_10	euxassay_005277_11	euxassay_005277_12	euxassay_005277_13	euxassay_005277_14

euxassay_005277_15	euxassay_005277_16	euxassay_005277_17	euxassay_005277_18	euxassay_005277_19

euxassay_005277_20	euxassay_005277_21	euxassay_005277_22	euxassay_005277_23	euxassay_005277_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7666_wholemount_strong.wlz

7666_wholemount_moderate.wlz

7666_wholemount_weak.wlz

7666_wholemount_possible.wlz

7666_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7666_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 05 06 21 22 moderate expression: see section 04 07 08 09 10 11 12 13 14 15 16 17 18 19 20 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 05 06 21 22 moderate expression: see section 04 07 08 09 10 11 12 13 14 15 16 17 18 19 20 23 24

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 18 19 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 18 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 09 16 17

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 11 12 15 16 17 18

cervical ganglion

strong

regional

strong expression: see section 08 09 17 18

thoracic ganglion

strong

regional

strong expression: see section 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 16 17 18 19

neural retina

strong

regional

strong expression: see section 01 02 03 22 23 24

tongue muscle

moderate

regional

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8927
Entity Detected:	Reep1, receptor accessory protein 1 ( MGI:1098827)
Sequence:	sense strand is shown >T8927 CGGGAGCGCAGCACCGCCCGCAGCCCAGCAATCCGGGCCGCGCCAGCCCGCCCGCCCGCCATGGTGTCGT GGATCATCTCCAGGCTGGTGGTGCTTATATTTGGCACCCTTTATCCTGCATATTATTCATACAAGGCTGT GAAGTCCAAGGACATTAAAGAATATGTCAAATGGATGATGTATTGGATTATATTTGCCCTCTTCACCACG GCAGAGACGTTCACAGACATCTTCCTTTGCTGGTTTCCATTCTATTATGAACTAAAAATAGCGTTTGTAG CCTGGCTGCTGTCTCCCTATACAAAAGGATCCAGCCTCCTGTACAGGAAGTTTGTTCATCCCACATTGTC TTCAAAAGAAAAGGAAATCGATGACTGCCTGGTCCAAGCAAAAGATCGAAGCTATGACGCCCTTGTGCAC TTTGGGAAGCGGGGCTTGAATGTGGCAGCCACTGCAGCTGTGATGGCTGCCTCCAAGGGACAGGGTGCCT TGTCAGAGAGACTCCGGAGCTTCAGCATGCAGGACCTCACCACCATCAGGGGTGATGGTGCTCCTGCTCC CTCGGGCCCTCCTCCACCAGGGACTGGGCGGTCCAGCGGCAAACACAGCCAGCCCAAGATGTCCAGGAGT GCTTCTGAGAGTGCCGGCAGCTCGGGCACCGCCTAGAACCCTACGATCCCGCTTCAGGAAGAAAAGTACC TCATCCTCGGCCACTGAAACCACGTGAGATGAGCCAACAGCCCCGGATCCACACATGTCTGTCTCCTCTG CCTTAAAGAGCTAGTCACTAGTACCATGGAAGTCCGCAGGCTGGGTGTGCTTCGAGTGTGCAGCCCACGG ACATGGCCTTTACTCGCTCCCCTCTTCTGCCTTTAAGACTCTTCTGTTCTTTCCTTTTTGTTCCTGCTGG GGAGAGGCTAAAGGGAAAAGGTGGTGGTGGAGGGTGACCTTTACGTCTTCTGGGGTTAGTGATTTTCCCC AATTGGATTGTCATTGTGGCCGCAGTGTTTTTAGATCTATAAGCACTCTTTCCATGATGCTGCTGAGATG TACATTTGTAATTTGTTTGTTGCATAGTTTTTAGTCCTGTAAATGCAAACGACGTGATCTTTTTTAAAAA CCCTTTTGTTGTTCTTGGTACTAATACATTGGACTATGGTGTACATAGTTACAGCTTTTGGCTTAATGTA ATGAACTCGCAAGGGCTGCCAGAAGTTCTGGTGAGAGAGCTGCAAAAACAAAAATGTAGACAAATATTTT AATTCTAGGCAATGTCTCCAACTTCCTTACAAAGGCAGTGCCAGTAAGGCATCCCTTCTCCTGCAGGAAG GCGGTGGTGTGCTTCTGGCGGCTGAGTGGTTGGTAGGTCAGAGCGGAGGCAGGAGTGCAGTGTTCCAGCA ACAGGAAGAAAGTTGGTGGGCATCAGGGAAGAGGGAGGAAGAGTACGGCTTCTTCAGTTCTGGGTCCCTG GCTGTCCTAATAGGTTTGTTGTGCTGTACCTAATCTCACCCTTCCTGCCCTTTGGGGTCAAGAACAGAAG CTTCCGAGCCCTTTGGGGAGCACTGGCCCTGCTTCAGGCAGGCCAGCTCACCCTTACTTAGCACGGCCAT TTAGGGAATGAAGATCAGCTGCTGGCAGAATTGTGAGTATCTCAGAAGGGCTTGGCAAGAGTGTTTCTCC TTCAGGGACCAGCGCTGTGGCCGACTTCTCCAGGAGCCTACATCTGAGCACCTTGTTTTAGAGTACACAA GGCAAAAAGAAAAGTCTAGAACTAGCTTTTGTTACAGATGTGCTCAAAACACATGAGACACTCAAATCTC AAATGGTCGCCAGAAGGCAGGGTCTAGGGAGGACTGAGGCTAAGCCACAGGCAGCTGTGGCTCTTGCTCC TTTCTGGCTTGTGTGGAAGGTGAATGGGAAGGACTGACACTCATGCTCACTCCATGTCTTCGTGGATGTT TTATACAAGCCAGGACTGCAGTGAGAATTTTGACAAGTCTCTGACTTAGATAACATTGCGTTGAGGGAGC TGCCCTCTCCTCTGATAGCTGAGCCAAATTGTAGGGCTAGTCTGTGTGTGCGTAGAGTTGGTGTTTTCGT TCCATGGAATGCATGCAGCAGTCACAACCCAGTTACTCCTATTTGGATTATATTTGTTCACAGCTATGCC CTGAAGGCTAGAGAAATAGTGTTGTATACCACATAGAACTGTCAACTGACTGCAAACAGGCTCAATTACA AACCGCTCTGGTACCGAGCTATGCAAACATGCACTGGGAGACTGCTCCATCAGCTGGGCCTTGGCCATTT CACAAAGGGTTTCAGTTGCGAGTTCATTGCTGCCAGATACACCTAATGGAATTTTCCAAAGGTGATTTTG TATTTCAGAACTAACCCAAGAGCTGATGACTGACGGTAAATAGTCTGCCTGGGCCCCATATCCATCTGAA CCCATTAAGCAAAGCATCTGCCCTCTTCAAGCTCACCCCACAGCTCCAGACAAGAGAAGTGACACCCTTC AAACATACCAACGTGTCAGCTGGGTGTAGCCAGGATGAAGAGGAACAGGTGCAGGAAAGCCTGAGAGAAG CAGCTTTGCAGCTCCCCCTCCACCCCCCGAAATAATAAATGGCTTTAGAGACAGAGTTCAAGTCCTAGTG TATTTTCATCGTTTTACAACAGGAAGTCATAACACATCGAGGCTCTTGAGTTTGTACTCAGCCATGCCAA AACCCAGTTTGAAGCACTCCTCAGACATGCTGTATTACTTCTCGCTCAAGATTTAAAATATAAAGGTATC TAAATCGTTGTCTTATTGTAAATGTAACTCCTTTTCCTTCAAGTGTTGACTAAGTTGTTTCCTCTCTTAA AGACACTCACTGTACAGCCAAACACAGCTGTCTTACTTCTTGAGAACTGTGTTTACTTTTCTGACAAGCT GTGTATTTGTATGTAGACTGACATATGATGTGAATGACCTTGTATGTAGAAGTGCGGTGCTCTGTCTACG CAGGATAAATGAATGTTTCTAGTTCGCTGACTTAAGACACAGGCTGTTTACAAATCGCTAGCTCGAAGGT CTACTTGTCGCGGCTTTGAGTTACTCTCCATTTCTGCAGAGGTGAGGTGCAGTCTCCTGCTCATCAACTG GAGATTTAGTCATAATGCCTCCTGAGTTTGTGTGAATAGCTTGCTCACTTTGCTGGCCTTTGATATGTAT GTTCTCTGTGGTAAACTGTCCATGTGTTTGTATTAAGTGCTTATCAACCACAACCATCACGTAGCCATCC CAGTTCTTCACCTTGCACAGTATTTGAAGTATTAGACGTGGTGGAAGATGTGCTTCATCCTCTGACAGTG GACGCTCGAAGAGCTCATGTTCCAGATTGTGACTGTGCGCATTTCCTCTAGTTTCTTTCTGAAATCTCTC TATAAATGAGTACAATTAATAGTATACTGGACTGTGTATCTACAGGATGTAAATTGCAGTATTCCAAAGG CTGAAGCTCTGCTGTTTTGTTATAATGCCTGATAAACATCTTGAATAAAGTCTTAACATTTTTCTTTTAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417744 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417744 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7670 same embryo
	EMAGE:7668 same embryo
	EMAGE:7667 same embryo
	EMAGE:7669 same embryo
	EMAGE:7671 same embryo
	EurExpress:euxassay_005277 same experiment
	MGI:4827679 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source