EMAGE mouse gene expression spatial database

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EMAGE:7671

Mta2 metastasis-associated gene family, member 2 ( MGI:1346340)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005311. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005311_01	euxassay_005311_02	euxassay_005311_03	euxassay_005311_04

euxassay_005311_05	euxassay_005311_06	euxassay_005311_07	euxassay_005311_08	euxassay_005311_09

euxassay_005311_10	euxassay_005311_11	euxassay_005311_12	euxassay_005311_13	euxassay_005311_14

euxassay_005311_15	euxassay_005311_16	euxassay_005311_17	euxassay_005311_18	euxassay_005311_19

euxassay_005311_20	euxassay_005311_21	euxassay_005311_22	euxassay_005311_23	euxassay_005311_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7671_wholemount_strong.wlz

7671_wholemount_moderate.wlz

7671_wholemount_weak.wlz

7671_wholemount_possible.wlz

7671_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7671_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8941
Entity Detected:	Mta2, metastasis-associated gene family, member 2 ( MGI:1346340)
Sequence:	sense strand is shown >T8941 TGCTCTCCGACCTGAGCCGAGCGCTGGGCCTTGTTCTCCGGATCGGCCGAAGGTGTTCCGGAAGGAGGCG AACCCGGACGCGGGCAGGGCAAACCTTGCCAGCGGTTGGCACCGCACGACGTCTGCTGGGACTCGGTGAG GACGCTAGGAGCTGGAACCTCACTCCGGTCAGGCAGGAATCGAGCTCGTTCCGGAAGAAGCCGAGTAGAC CGGAGCTGGCCTCGGTGTCCCGGCGTGAGGCTATAGCAGTGTCGTCGTCGCGCGGGCAGTGATGAGCCGC GGCGGCTGAGCGAGCAGAGGTGCCGGCTGCTCGGGCCCCATCGACACTTCCGCGAAGCGTCAGCCACGCG CTGGGGGCTTCGTCTGGAGCTGAGCTTTGCCCCCGCGAGCCTCCGGGACCTCTTTGTGTGGCCGGAGGCG GCGGCGGGAACGGCCATGGCGGCTAACATGTACCGGGTGGGAGATTACGTCTATTTTGAGAACTCCTCCA GCAATCCTTACCTGGTTAGACGGATTGAGGAGCTCAATAAGACTGCAAATGGAAATGTGGAAGCAAAGGT TGTCTGTCTTTTCCGGCGAAGGGATATTTCTAGTAGCCTCAACAGCTTGGCTGACAGTAATGCTCGGGAG TTTGAGGAGGAATCAAAACAGCCAGGGGTCTCCGAGCAGCAGAGACATCAACTGAAGCACCGAGAGCTTT TTCTTTCTCGGCAGTTTGAATCATTACCAGCCACCCACATAAGGGGGAAGTGCAGCGTGACTCTCTTGAA CGAGACAGACATCTTAAACCAGTATCTAGACAAGGAGGACTGCTTTTTTTACTCACTGGTGTTTGATCCT GTGCAGAAGACACTTCTGGCTGATCAAGGGGAGATCAGAGTTGGTTGCAAATTCCAAGCTGAGATCCCAG ATCGTTTGGCAGAGGGAGAATCAGATAATCGAAACCAACAGAAGATGGAGATGAAAGTCTGGGACCCAGA CAACCCTCTCACAGACCGGCAGATTGATCAGTTTCTCGTGGTGGCCCGAGCTGTGGGAACCTTCGCGAGA GCCCTAGACTGCAGCAGCTCAATTCGGCAGCCAAGCTTGCACATGAGTGCAGCTGCTGCGTCCCGAGATA TCACTCTGTTCCATGCCATGGATACCTTGCAAAGGAATGGCTATGACTTGGCCAAGGCTATGTCAACCCT AGTACCACAGGGAGGCCCGGTGCTCTGTCGGGATGAGATGGAGGAATGGTCTGCTTCTGAAGCCATGCTG TTTGAAGAGGCTCTGGAGAAGTACGGGAAGGATTTCAATGATATCCGCCAGGACTTTCTACCCTGGAAGT CACTTGCAAGCATAGTCCAGTTTTATTACATGTGGAAAACCACAGACCGGTATATTCAGCAGAAAAGATT GAAAGCTGCTGAAGCAGACAGCAAACTGAAACAGGTCTACATCCCCACTTATACCAAGCCAAACCCTAAC CAGATCATTTCTGTGGGTTCAAAACCTGGCATGAATGGGGCTGGATTCCAGAAGGGCCTGACTTGTGAGA GCTGCCACACCACACAGTCTGCCCAGTGGTATGCCTGGGGACCCCCTAACATGCAGTGCCGACTTTGTGC TTCCTGTTGGATTTATTGGAAGAAATACGGGGGACTGAAGACCCCTACCCAACTTGAAGGGGCTGCTCGG GGCACCACAGAGCCACACTCAAGAGGTCATTTATCCAGACCTGAAGCCCAAAGTCTCTCCCCCTATACAA CCAGTGCTAACCGGGCCAAGTTGCTGGCTAAGAACAGGCAAACTTTCTTGCTCCAGACCACAAAGCTAAC TCGTCTAGCCAGACGAATGTGCCGAGATCTATTACAGCCAAGGAGGGCTGCCCGAAGACCCTATGCACCT ATCAATGCCAATGCCATCAAGGCAGAGTGCTCGATCCGACTTCCTAAGGCTGCCAAGACGCCACTGAAAA TTCACCCTCTGGTGCGGCTGCCCTTGGCAACTATCGTCAAAGATCTGGTGGCTCAGGCACCTCTGAAACC AAAAACACCTCGGGGTACCAAGACACCAATCAACAGAAACCAGCTGACCCAGAATCGAGGCCTGGGGGGC ATTATGGTGAAACGTTCCTATGAGACTATGGCAGGGGCAGGGGTCCCCTTCTCTGCCAATGGAAGGCCTC TGGCTTCAGGGATTCGTTCAAGCTCACAGCCAGCAGCCAAGCGTCAGAAACTAAACCCTGCTGATGCTCC CAATCCAGTGGTATTTGTGGCCACAAAGGACACCAGGGCTTTAAGGAAAGCTCTAACTCACCTGGAAATG CGCCGAGCGGCCCGAAGGCCCAACCTACCCCTGAAGGTGAAACCAACGCTGATGACAGTGCGGCCCCCAG TTCCTCTGCCCGCTTCTTCACATCCTGCCAGCACCAATGAGCCCATTGTCCTGGAGGATTGAGCATCTTA GGGGAAGGGAGGTGGGATGAGAGGTAGAGGGGTGGGTGCCCAGGACATACAAATCTTCATTCCTTTTGGT ATCAAAGGGAGTAAGGAGTGAGGGAGGGTTGACCCTGGTGTGTGTGTGTCCTTGCAGGGGTTGGACACCC TCTGGTGTTAGTTACTGCCTGTAACTGCCAGTAACGTCCCTTTTTGGGCTTCACAGCCTATAGGAACTTG CCTGTGTGTGTACCTACCCTATTTGGGGCCTGGGACCCACAGGTGGTTTGGACAGTTGGGGGGGAGGGTT TTTTAATTTTTTAAAAATGAACCTCCCTTTGTAAAAGGGATGGAGGAGGGGAAGAGTAAACATATCAGGT GTTAGTCCCTGGCAGGGGAAGGGAAGTACACTGCCATGTCTCATTTTTCCTTATTTTCTAATGGGGGGTT ACTCTTACTGTCCGGTGTCCGGACTTTCCTAATTGGAATTTGAGAGCCCTAAGATGGCGTCAACCCCAGC CCATGCTCACTCTTTCTTCCCTCCCCTTTCTCCCTCTGCCTTTTATATGCCAATTCTCAGAAATAAAGAT CTTTTGTCTGTTTTTTAACCTCAGATTCTGTAATTGGTTCTTACAGTGACAAATAAAAAGCTGTTTTCTT CAGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6419411 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419411 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7666 same embryo
	EMAGE:7670 same embryo
	EMAGE:7668 same embryo
	EMAGE:7667 same embryo
	EMAGE:7669 same embryo
	EurExpress:euxassay_005311 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source