EMAGE mouse gene expression spatial database

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EMAGE:7672

Inf2 inverted formin, FH2 and WH2 domain containing ( MGI:1917685)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005269. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005269_01	euxassay_005269_02	euxassay_005269_03	euxassay_005269_04

euxassay_005269_05	euxassay_005269_06	euxassay_005269_07	euxassay_005269_08	euxassay_005269_09

euxassay_005269_10	euxassay_005269_11	euxassay_005269_12	euxassay_005269_13	euxassay_005269_14

euxassay_005269_15	euxassay_005269_16	euxassay_005269_17	euxassay_005269_18	euxassay_005269_19

euxassay_005269_20	euxassay_005269_21	euxassay_005269_22	euxassay_005269_23	euxassay_005269_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7672_wholemount_strong.wlz

7672_wholemount_moderate.wlz

7672_wholemount_weak.wlz

7672_wholemount_possible.wlz

7672_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7672_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20

cerebral cortex mantle layer

strong

regional

strong expression: see section 08 09 moderate expression: see section 05 06 07 10 11 13 14 15 16 17 18 weak expression: see section 03 04 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 06 weak expression: see section 03 04 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8911
Entity Detected:	Inf2, inverted formin, FH2 and WH2 domain containing ( MGI:1917685)
Sequence:	sense strand is shown >T8911 ACCCAGAGCAGGCCAGATACCTCCACCTCCTCCCCTGCCTGGTTTCTCTGTCCCCTCCATGATGGGTGGT GTGGAGGAGATCATTGTGGCCCAGGTCGACCACAGCCTGGGCTCAGCCTGGGTCCCCAGTCACCGCAGAG TGAATCCACCCACACTGAGAATGAAGAAGCTGAACTGGCAGAAGCTGCCATCCAATGTGGCACGAGAGCG CAACTCCATGTGGGCCACACTGGGCAGCCCCTGCACAGCCGCAGTGGAGCCCGACTTCTCCAGCATTGAG CAGCTGTTCTCCTTCCCCACGGCTAAGCCCAAGGAGCCCTCTGCTGCCCCCGCCAGGAAGGAGCCCAAAG AAGTCACTTTTCTGGACTCCAAGAAGAGCCTGAACCTCAACATCTTCCTGAAGCAATTTAAATGTTCCAA TGAAGAAGTCACCAGCATGATCCAGGCTGGAGACACCAGCAAGTTTGATGTGGAGGTCCTCAAACAGCTT CTAAAGCTTCTTCCAGAGAAGCATGAGATTGAGAACCTACGGGCGTTCACTGAGGAGCGAGCCAAGTTGT CCAATGCTGACCAGTTCTATGTTCTGCTCCTGGACATTCCCTGCTACCCGCTGCGGGTGGAGTGCATGAT GCTGTGTGAGGGAACGGCCATCGTCCTGGACATGGTGCGGCCCAAGGCCCAGCTGGTGCTCACTGCCTGC GAGAGCCTGCTCACCAGCCAGCGGTTGCCAGTCTTCTGTCAGCTGATCCTCAAAATCGGGAATTTCCTCA ACTACGGCAGCCACACGGGAGATGCAGATGGTTTCAAGATTAGCACCTTGCTGAAGCTCACAGAGACCAA GTCCCAGCAGAGTCGCGTGACGCTGCTGCACCACGTGCTGGAGGAAGTGGAGAAGAGCCACCCGGACCTC CTACAGCTATCCCGGGACCTGGAGCCACCCTCTCAAGCTGCAGGAATCAACGTGGAGATCATCCACTCAG AGGCCAGTGCCAACCTGAAGAAGCTTCTAGAAGCGGAGCGGAAGGTGTCCGCTTCCATCCCTGAGGTGCA GAAGCAGTACGCTGAGCGCCTCCAGGCCAGCATTGAGGCCTCTCAGGAGCTGGACAAGGTATTCGACGCC ATTGAGCAAAAGAAACTGGAGCTGGCTGACTACCTGTGCGAAGACCCCCAGCAGCTGTCCCTAGAGGACA CGTTCAGCACCATGAAGACCTTCCGAGACCTCTTCACCCGTGCCCTGAAGGAGAACAAGGACCGGAAGGA ACAGATGGCAAAAGCGGAAAGGAGGAAACAGCAGCTGGCGGAGGAGGAGGCCCGGAGGCCGAGGGACGAG GATGGGAAGCCTATCAGGAAGGGGCCTGGGAAGCAAGAAGAGGTGTGCGTCATCGATGCCCTGCTGGCCG ACATCAGGAAGGGCTTCCAGCTACGGAAGACAGCCCGGGGCCGGGGAGACACTGAAGCAAGTGGCAGAGT GGCCCCCGCAGATCCTCCAAAGGCCACAGAGCCTGCAACTGCCAGCAATCCTACCCAGGGCACAAACCAC CCTGCTTCAGAGCCCTTGGATACCACAGCAGCTGACGAGCCGCAGGGCTGGGACCTTGTGGATGCTGTAA CCCCCAGCCCCCAGCCCTCCAAGGAGGAAGATGGTCCTCCAGCCCTGGAGAGGCGCTCCTCCTGGTATGT GGATGCCATTGACTTCCTGGACCCTGAAGATACCCCGGATGCCCAGCCCTCTGAGGGGGTCTGGCCAGTG ACTCTGGGAGATGGTCAAGCTCTGAATCCCCTCGAGTTCTCTAGCAATAAGCCTCCTGGAGTCAAGAGCT CGCACCAGGACGCTACGGATCCTGAAGCCTTGTGGGGCGTCCACCGAACCGAGGCAGACAGCACCAGTGA GGGACCAGAGGATGAAGCTCAGCGAGGACAGAGCACCCACCTACCCCGCACAGGCCCCGGAGAGGATGAG GATGGAGAGGACACAGCCCCAGAATCTGCACTTGACACGTCCCTGGACAGGTCCTTCTCTGAGGACGCAG TGACAGATTCCTCAGGGTCTGGCACCCTCCCCAGGGTCCAAGGCCGGGTCTCAAAGGGGACGAGCAAGCG GAGGAAGAAACGTCCCTCAAGGAACCAAGAAGGCCTCAGGTCCAGGCCCAAAGCCAAGTGAGTGCTCTCG TCGGCTCAGCACATCGGCTGGATGTGTTGCCTGGAGAGGCTCTTCGGGGCCAGGCTGGGACTGGGCCCCC GAGAAACCCAAACTCCGTGCCTTACCCAGCCAGGATCCTTGGGAGCCTTGGGCATTGATGCTGGGACCTG GCCTGGCACCCTGGGCCACCTGCACGCTCTTAGCCCCTGCTTTTGCAAACCACTCCCACCCCCTATCGCA TGCACCACAGGGGTGGACGGGAGCCTCCTGCCTGGCTCTAGGGAGATGGATCACAGACACCTGCACTGGG GCTGAAACATCCCCTCTCCCCCAACCCCCATGGCTCAGTTGATTTTTAGCTGAGTGCTGTTTTCTGCATC TTTATTCTGCTATGGCTGAGTTGAGGTTAGTGAGGGACTGAGGGAATGCTCGTTGGAAGTGCTGTGGGTA GGACCTGGCAGGTAGGCTCTAGGGAACAAATAAACAAAAAGTTCATGTGCACAATGAGTGGTGACCAGAC CACTGCAGCTGTCATCTCCCTTGGTGTGTGCCCAGTGGGGAACAAGCTGCCCCTGCTCAGCCCCTCTCAT GTGTGCACTGTTGAGTCTGCAGGCCAGCAGGGATTTTAACCAATAAAGAGCAACCTTGTCTGTCAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416724 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416724 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7674 same embryo
	EMAGE:7675 same embryo
	EMAGE:7677 same embryo
	EMAGE:7673 same embryo
	EMAGE:7676 same embryo
	EurExpress:euxassay_005269 same experiment
	MGI:4825590 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source