EMAGE mouse gene expression spatial database

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EMAGE:7675

Herc4 hect domain and RLD 4 ( MGI:1914595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005281. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005281_01	euxassay_005281_02	euxassay_005281_03	euxassay_005281_04

euxassay_005281_05	euxassay_005281_06	euxassay_005281_07	euxassay_005281_08	euxassay_005281_09

euxassay_005281_10	euxassay_005281_11	euxassay_005281_12	euxassay_005281_13	euxassay_005281_14

euxassay_005281_15	euxassay_005281_16	euxassay_005281_17	euxassay_005281_18	euxassay_005281_19

euxassay_005281_20	euxassay_005281_21	euxassay_005281_22	euxassay_005281_23	euxassay_005281_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7675_wholemount_strong.wlz

7675_wholemount_moderate.wlz

7675_wholemount_weak.wlz

7675_wholemount_possible.wlz

7675_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7675_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8914
Entity Detected:	Herc4, hect domain and RLD 4 ( MGI:1914595)
Sequence:	sense strand is shown >T8914 GTTTCACGGGCGAGCCGGGCAGCAGAGGCAGCGGAGCTGCGGTCGGGGACGTCGGAGGAGCCGTGGTAAT CGCCGCGGCCGGCTGAAGGAGGAAGGTCTTGGGAGCTGGAGAGCCGCCCAGAGGTCCAGGGAGCGTCCGT CCTCCATCGCTTAGGGCTCTCCCTGCCCTGAGAGACCCGATAAGAACTTCCATTTTCTGTTGATGTGGAG ACATAGGGTACCTCCCAGTTTCCAGTGTTTCAGAAGGGACTTTTGTATTGAAACTGGAAGACCAAGGTCA TTCCAAGCATGTTGTGCTGGGGCAATGCATCCTATGGACAACTAGGTTTGGGAGGAATTGATGAAGAAAT TGTACTAGAGCCCAGGAGAAGTGACTTTTTCGTGAACAAAAAGGTCCGAGATGTAGGCTGTGGACTCAGG CACACTGTGTTTGTCCTGGATGATGGGACTGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTC ATGAAAAGTCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAGAATATCGTAGCTGTTGCGTG TGGAGAAGCTCACACGTTAGCGCTGAATGACAAGGGCCAGGTGTATGCTTGGGGTCTCGACTCTGACGGA CAGCTTGGCCTACAGGGATCAGAGGAATGTATCAGAGTACCCAGAAATATTAAAAGTCTTTCGGATATCC AGATAGTACAGGTTGCGTGTGGTTACTATCATTCGCTTGCACTTTCTAAAGCAAGTGAAGTTTTCTGTTG GGGACAGAATAAATATGGCCAGCTGGGTCTAGGCATTGATTGTCAAAAGCAAACTTCACCACAGCTGATT AAGTCTTTGCTTGGAATACCATTCATGCAAGTCGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTT CCGGAGCTATCTTTGGATGGGGACGTAACAAATTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTA TGTTCCTAATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGCGGAGAAGATCATACT GCTGCATTAACCAAGGAAGGTGGAGTGTTTACCTTTGGAGCTGGGGGCTATGGCCAGCTGGGTCATAATT CTACCAGTCATGAGATAAATCCCAGGAAAGTTTTTGAACTCATGGGAAGCATTGTTACTCAGGTCGCTTG TGGAAGGCAGCATACCTCTGCATTTGTTCCTTCTTCAGGACGAATTTACTCCTTTGGTCTTGGTGGTAAT GGGCAGTTGGGAACTGGTTCAACCAGCAACAGAAAAAGCCCTTTCACTGTAAAAGGAAACTGGTTTTCTT ATAATGGACAGTGTCCACAAGATATTGGTTCTGAAGACTATTTCTGTGTCAAAAGAATTTTCTCAGGTGG AGATCAAAGCTTTTCACATTACTCTAGTCCTCAGAACTGTGGACCTCCAGATGACTTTAGATGCTCCGAC CCTTCCAAGCAGATCTGGACAGTGAACGAAGCTCTGATTCAGAAATGGCTCAGCTACCCCTCTGGAAGGT TCCCTGTGGAGATAGCCAATGAGATAGATGGAACATTTTCTTCATCTGGCTGCCTAAATGGAAGTTTTCT GGCTATTAGCAATGACGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAACGCTGCTAGA CTCTTATTCCACAAACTTATACAACCTGATCATCCTCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAA AGAATCTAATCCCTAAACTGACTAGTTCCCTACCTGATGTTGAAGCTCTGAGGTTTTATCTTACCCTGCC AGAGTGTCCCCTGATGAGTGACTGCAACAATTTCACAACAATAGCAATCCCCTTTGGTACAGCTCTTGTG AACCTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCA AGATAGTAGAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGCATTCCCCCTTC TGAAAGAAGAATTTTCAACAGTTTTCTTCATACCGCATTAAAGGTTTTAGAAATATTGCATAGGGTAAAT GAGAAAACAGGACAACTTATTCAATATGACAAGTTTTACATCCATGAAGTCCAAGAGCTGATAGACATAA GGAATGACTATATCAACTGGGTCCAGCAGCAAGCCTATGGAGTGTTGGCAGATATCCCGGTGACAATTTG TACATATCCATTTGTATTTGATGCCCAGGCAAAAACTACTCTGCTCCAGACAGATGCAGTCTTGCAGATG CAGATGGCCATTGACCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATCGAGTCTGTGA ATCCTTGCTTAATTCTAGTTGTTCGCAGAGAGAATATTGTAGGAGATGCGATGGAAGTCCTCAGGAAAAC CAAGAATATAGATTATAAAAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTTGATGCAGGAGGT GTTCGCAAAGAATTTTTCTTGCTTATCATGAGGGAATTGTTGGATCCTAAATACGGCATGTTTCGATATT ATGAAGATTCCAGGCTCATTTGGTTTTCAGATAAGACATTTGAAGACAGTGATTTGTTCCACTTGATCGG TGTTATCTGTGGATTAGCAATTTATAATTTTACCATTGTGGACCTCCATTTCCCTTTGGCTTTATATAAG AAACTGCTGAAAAGGAAGCCGTCCCTGGATGATCTGAAAGAGCTGATGCCAGCCGTCGGGAGAAGCATGC AGCAATTGCTGGACTATCCAGAAGATGACATAGAGGAAACATTTTGTCTAAACTTTACGATCACAGTTGA GAACTTCGGTGCAACAGAAGTAAAAGAGCTGGTTCTGAACGGGGCAGACACTGCTGTTAATAGACAGAAT CGGCAGGAGTTTGTTGATGCCTATGTGGATTACATATTCAATAAATCAGTGGCATCTTTATTTGATGCTT TCCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTTCTTCTGCTCTTCCAGCCTAATGAATTACAAGC TATGGTTATTGGGAATACAAATTATGATTGGAAAGAACTGGAGAAGAATACAGAATACAAAGGAGAGTAT TGGGCAGACCATCCCACGATAAAAATTTTTTGGGAAGTGTTTCATGAATTACCACTAGAGAAGAAGAAAC AATTTCTATTATTTTTGACAGGTAGTGATCGGATTCCCATTCTTGGTATGAAGAGTCTGAAACTAGTCAT CCAGTCAACAGGAGGTGGTGAGAGCTATCTCCCGGTTTCCCATACCTGTTTTAATCTTCTAGATCTCCCA AAATATACAGAGAAAGAAACCCTCCGATGTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTT TAATATGACCTTGGACTTTTCTGTTTAGTACAAAAACCACTCGTGTTTCTCTTGTGAATTCAGCACAGTG AGAGTTACTTCTGTAATTCTTACTTATAAATTGTTCACTACTGTACTGATGAAAGCCCAAAATTAAAAAC TGTGCATTAATACTAAACTGTACAGAACCATGAATTGTCCATCCTCATACAAGTATTGTGAATACATTAA AGTTTTACTCACAGGAATTTTAAGAGTTTGCATATTTCGGAAATTACCTGATGTGTGGGTGCATGGAAAT GTTGCTTACTGTTTCTTCAATCACTGGGGGGAAAGACTACGATGGCCTGCCCACAGTCATTTGATTATTT TTCCATTTTGTAAATAAAAATTTTGTAATAAAATAGTGACATTCAGAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417111 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417111 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7672 same embryo
	EMAGE:7674 same embryo
	EMAGE:7677 same embryo
	EMAGE:7673 same embryo
	EMAGE:7676 same embryo
	EurExpress:euxassay_005281 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source